Differential localization of the γ3 and γ12 subunits of G proteins in the mammalian brain

Journal of Neurochemistry

Volumn 68, Issue 2, 1997, Pages 820-827

  Morishita, Rika ^a   Saga, Shinsuke ^a   Kawamura, Noriko ^a   Hashizume, Yoshio ^b   Inagaki, Toshiaki ^c   Kato, Kanefusa ^a   Asano, Tomiko ^a  

^a INSTITUTE FOR DEVELOPMENTAL RESEARCH   (Japan)

^b AICHI MEDICAL UNIVERSITY   (Japan)

^c Geriatric Hospital   (Japan)

Author keywords

Aging; G protein; Localization; Reactive astrocytes; subunit

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN SUBUNIT;

AGING; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN DEVELOPMENT; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MALE; NONHUMAN; PHEOCHROMOCYTOMA CELL; POSTNATAL DEVELOPMENT; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; PYRAMIDAL NERVE CELL; RAT; TISSUE DISTRIBUTION;

EID: 0031030443     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.68020820.x     Document Type: Article

References (28)

1. o in rat brain and heart. J. Neurochem. 51, 1711-1716.
2. Asano T., Morishita R., Matsuda T., Fukada Y., Yoshizawa T., and Kato K. (1993) Purification of four forms of the βγ subunit complex of G proteins containing different γ subunits. J. Biol. Chem. 268, 20512-20519.
3. Asano T., Morishita R., Ohashi K., Nagahama M., Miyake T., and Kato K. (1995) Localization of various forms of the γ subunit of G protein in neural and nonneural tissues. J. Neurochem. 64, 1267-1273.
4. Cali J. J., Balcueva E. A., Rybalkin I., and Robishaw J. D. (1992) Selective tissue distribution of G protein γ subunits, including a new form of the γ subunits identified by cDNA cloning. J. Biol. Chem. 267, 24023-24027.
5. Curren R. C. and Gregory J. (1978) Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections. J. Clin. Pathol. 31, 974-983.
6. 5). Mol. Cell. Biol. 12, 1585-1591.
7. A receptor gene expression in adult rat hippocampus: an in situ hybridization study. J. Neurosci. 14, 2697-2707.
8. Gautam N., Baetscher M., Aebersold R., and Simon M. I. (1989) A G protein gamma subunit shares homology with ras proteins. Science 244, 971-974.
9. Gautam N., Northup J., Tamir H., and Simon M. I. (1990) G protein diversity is increased by associations with a variety of γ subunits. Proc. Natl. Acad. Sci. USA 87, 7973-7977.
10. Hurley J. B., Fong H. K. W., Teplow D. B., Dreyer W. J., and Simon M. I. (1984) Isolation and characterization of a cDNA clone for the γ subunit of bovine retinal transducin. Proc. Natl. Acad. Sci. USA 81, 6948-6952.
11. Iñiguez-Lluhi J., Kleuss C., and Gilman A. G. (1993) The importance of G-protein βγ subunits. Trends Cell Biol. 3, 230-236.
12. Laemmli U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
13. Morishita R., Fukada Y., Kokame K., Yoshizawa T., Masuda K., Niwa M., Kato K., and Asano T. (1992) Identification and isolation of common and tissue-specific geranylgeranylated γ subunits of guanine-nucleotide-binding regulatory proteins in various tissues. Eur. J. Biochem. 210, 1061-1069.
14. 12, and its phosphorylation by protein kinase C. J. Biol. Chem. 270, 29469-29475.
15. Mountjoy C. Q., Roth M., Evans N. J. R., and Evans H. M. (1983) Cortical neuronal counts in normal elderly controls and demented patients. Neurobiol. Aging 4, 1-11.
16. Nadler J. V., Perry B. W., and Cotman C. W. (1978) Intraventricular kainic acid preferentially destroys hippocampal pyramidal cells. Nature 271, 676-677.
17. Nakane P. (1975) Recent progress in peroxidase-labeled antibody method. Ann. NY Acad. Sci. 254, 203-211.
18. Neer E. J. (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80, 249-257.
19. Ong O. C., Yamane H. K., Phan K. B., Fong H. K. W., Bok D., Lee R. H., and Fung B. K.-K. (1995) Molecular cloning and characterization of the G protein γ subunit of cone photoreceptors. J. Biol. Chem. 270, 8495-8500.
20. 11 subunits. J. Biol. Chem. 270, 21765-21771.
21. Robishaw J. D., Kaiman V. K., Moomaw C. R., and Slaughter C. A. (1989) Existence of two γ subunits of the G proteins in brain. J. Biol. Chem. 264, 15758-15761.
22. γ8, is expressed during neurogenesis ini the olfactory and vomeronasal neuroepithelia. J. Biol. Chem. 270, 6757-6767.
23. Sato T., Ito, Y., Mizuno T., Tauchi H., Inagaki T., and Yamamoto T. (1989) Morphological studies of the hippocampus of 100 elderly females in relation to age and grade of dementia. (Abstract in English). Jpn. J. Geriatr. 26, 594-601.
24. Schaffner W. and Weissmann C. (1973) A rapid, sensitive, and specific method for the determination of protein in dilute solution Anal. Biochem. 56, 502-514.
25. Schägger H. and von Jagow G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379.
26. Schnitzer J., France W. W., and Schachner M. (1981) Immunocytochemical demonstration of vimentin in astrocytes and ependmal cells of developing and adult mouse nervous system. J. Cell Biol. 90, 435-447.
27. Weir M. D., Patel A. J., Hunt A., and Thomas D. G. T. (1984) Developmental, changes in the amount of glial fibrillary acidic protein in three regions of the rat brain. Dev. Brain Res. 15, 147-154.
28. Yatsunami K., Pandya B. V., Oprian D. D., and Khorana H. G. (1985) cDNA-derived amino acid sequence of the γ subunit of GTPase from bovine rod outer segments. Proc. Natl. Acad. Sci. USA 82, 1936-1940.