Crystallization and preliminary structural analysis of Bacillus subtilis adenylosuccinate lyase, an enzyme implicated in infantile autism

Protein Science

Volumn 5, Issue 4, 1996, Pages 786-788

  Redinbo, Matthew R ^a,c   Eide, Sithumini M ^b,d   Stone, Randy L ^b,e   Dixon, Jack E ^b   Yeates, Todd O ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

^d Janssen Pharmaceuticals   (United States)

^e PFIZER INC   (United States)

Author keywords

autism; fumarase; HIV therapeutics; purine biosynthesis; X ray crystallography

Indexed keywords

ADENYLOSUCCINATE LYASE; FUMARATE HYDRATASE;

BACILLUS SUBTILIS; CRYSTALLIZATION; ENZYME STRUCTURE; INFANTILE AUTISM; MENTAL DEFICIENCY; PRIORITY JOURNAL; PURINE SYNTHESIS; REVIEW; STRUCTURE ANALYSIS; X RAY ANALYSIS;

EID: 0029877127     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050425     Document Type: Review

References (20)

1. Aimi J, Badylak J, Williams J, Chen Z, Zalkin H, Dixon JE. 1990. Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli. J Biol Chem 265:9011-9014.
2. Becker MA, Losman MJ, Rosenberg AL, Mehlman I, Levinson DJ, Holmes EW. 1986. Phosphoribosylpyrophosphate synthetase superactivity. A study of five patients with catalytic defects in the enzyme. Arthritis Rheum 29:880-888.
3. Ebbole DJ, Zalkin H. 1987. Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J Biol Chem 262:8274-8287.
4. Jaeken J, Van den Berghe G. 1984. An infantile autistic syndrome characterized by the presence of succinylpurines in body fluids. Lancet ii: 1058-1061.
5. Jaeken J, Wadman SK, Duran M, van Sprang FJ, Beemer FA, Holl RA, Theunissen PM, de Cock P, Van den Berghe F, Van den Berghe G. 1988. Adenylosuccinase deficiency: An inborn error of purine nucleotide synthesis. Eur J Pediatr 148:126-131.
6. Matthews BW. 1968. Solvent content of protein crystals. J Mol Biol 33:491-497.
7. Mitsuya H, Broder S. 1986. Inhibition of the in vitro infectivity and cytopathic effect of human T-lymphotrophic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) by 2′,3′-dideoxynucleosides. Proc Natl Acad Sci USA 83:1911-1915.
8. Mitsuya H, Yarchoan R, Broder S. 1990. Molecular targets for AIDS therapy. Science 249:1533-1544.
9. Moyle GJ, Nelson MR, Hawkins D, Gazzard BG. 1993. The use and toxicity of didanosine (ddI) in HIV antibody-positive individuals intolerant to zidovudine (AZT). Quart J Medicine 86:155-163.
10. Nair V, Sells TB. 1992. Interpretation of the roles of adenylosuccinate lyase and of AMP deaminase in the anti-HIV activity of 2′,3′-dideoxyadenine and 2′,3′-dideoxyinosine. Biochim Biophys Acta 1119:201-204.
11. Navaza J. 1994. AMoRe: An automated package for molecular replacement. Acta Crystallogr A 50:157-163.
12. Otwinowski Z, Minor W. 1995. The HKL manual. New Haven, Connecticut: Yale University.
13. Patterson D, Graw S, Jones C. 1981. Demonstration, by somatic cell genetics, of coordinate regulation of genes for two enzymes of purine synthesis assigned to human chromosome 21. Proc Natl Acad Sci USA 78:405-409.
14. Seegmiller JE. 1980. Diseases of purine and pyrimidine metabolism. In: Bondy PK, Rosenberg LE, eds. Metabolic control and disease. Philadelphia, Pennsylvania: WB Saunders. pp 777-937.
15. Simpson A, Bateman O, Driessen H, Lindley P, Moss A, Mylvaganam S, Narebor E, Slingsby C. 1994. The structure of avian eye lens δ-crystallin reveals a new fold for a superfamily of oligomeric enzymes. Nature Struct Biol 1:724-734.
16. Stone RL, Aimi J, Barshop BA, Jaeken J, Van den Berghe G, Zalkin H, Dixon JE. 1992. A mutation in adenylosuccinate lyase associated with mental retardation and autistic features. Nature Genetics 1:59-63.
17. Stone RL, Zalkin H, Dixon JE. 1993. Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase. J Biol Chem 268:19710-19716.
18. Tabor S, Richardson CC. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82:1074-1078.
19. Weaver TM, Levitt DG, Donnelly MI, Wilkens Stevens PP, Banaszak LJ. 1995. The multisubunit active site of fumarase C from Escherichia coli. Nature Struct Biol 2:654-662.
20. Yamaguchi H, Kato H, Hata Y, Nishioka T, Kimura A, Oda J, Katsube Y. 1993. Three-dimensional structure of the glutathione synthetase from Escherichia coli at 2.0 Å resolution. J Mol Biol 229:1083-1100.