Membrane phospholipid control of nucleotide sensitivity of K(ATP) channels

Science

Volumn 282, Issue 5391, 1998, Pages 1138-1141

  Shyng, S L ^a   Nichols, C G ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; MEMBRANE PHOSPHOLIPID; NUCLEOTIDE; POLYPHOSPHOINOSITIDE; POTASSIUM CHANNEL;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CHANNEL GATING; CONTROLLED STUDY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 0032491439     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5391.1138     Document Type: Article

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13. 2 (Fig. 1, A and B). Inward currents (shown as upward deflections) were measured at -50 mV, filtered at 0.5 to 3 kHz, digitized at 22 kHz (Neurocorder, Neurodata, NY,), and stored on videotape for offline analysis with the Microsoft Excel and Solver programs. Data are presented as the mean ± the standard error of the mean.
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19. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
20. ir6.2-C, expressed in Escherichia coli, were purified with glutathione-agarose beads and dialysis in K-INT solution (7)
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25. ir6.2 and dissociated ventricular myocytes, respectively. This work was supported by grant HL45742 from NIH (C.G.N.), an Established Investigatorship from the American Heart Association (C.C.N.), a Career Development Award from the American Diabetes Association (S.L.S.), and by the Washington University Diabetes Research Training Center.