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2 was from Calbiochem, and all other phospholipids were from Avanti Polar Lipids (Alabaster, AL). PI-PLC (from Bacillus cereus) was from Calbiochem.
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Exchange current was recorded in 22 excised patches, 7 of which disrupted before completion of the experiment. Results from the nine stable control patches and six stable PI-PLC-treated patches were compared by Student's t tests. The average peak current after 4 min of recording was 67 ± 7 pA (SEM) in control patches and 51 ± 9 pA in PI-PLC treated patches. The difference was not significant (P > 0.1). During application of ATP, exchange current increased by 4.7 ± 0.9 times in control patches to 88 ± 14 pA and by 0.19 ± 0.05 times in treated patches to 14 ± 2 pA. The differences were highly significant (P < 0.001).
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2+ were small or absent. ATP effects were restored by PI.
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26
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note
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2+.
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27
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34
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9444272389
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note
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3 was added with twofold NaOH to compensate for protons released from EGTA. Fluorescence of ANEPPS increased in response to aluminum; fluorescence of rhodamine-labeled hexalysine decreased upon binding to vesicles, and the decrease was reversed by aluminum.
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We thank P. C. Sternweis, S. Muallem, and H. L. Yin for insightful discussions, encouragement, and reagents; and S. Feng and X. Xu for technical help. Supported by grants from NIH (5-R1-HL51323-03) and the American Heart Association (95014830) to D.W.H. and from NIH (GM49993) to P.C. Sternweis.
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