Genetic engineering, production and characterisation of monomeric variants of the dimeric Serratia marcescens endonuclease

FEBS Letters

Volumn 425, Issue 3, 1998, Pages 517-522

  Franke, Ingo ^a   Meiss, Gregor ^a   Blecher, Dinah ^a   Gimadutdinow, Oleg ^b   Urbanke, Claus ^c   Pingoud, Alfred ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b KAZAN FEDERAL UNIVERSITY   (Russian Federation)

^c HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Circular dichroism; Folding; Quaternary structure; Site directed mutagenesis; Stability

Indexed keywords

DIMER; ENDONUCLEASE;

ARTICLE; ENZYME ACTIVITY; ENZYME STRUCTURE; GENETIC ENGINEERING; MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN VARIANT; SERRATIA MARCESCENS;

BACTERIAL PROTEINS; CATALYSIS; CIRCULAR DICHROISM; DIMERIZATION; DNA; ENDONUCLEASES; ENZYME STABILITY; GENETIC ENGINEERING; GUANIDINE; HYDROGEN BONDING; KINETICS; MOLECULAR WEIGHT; MUTAGENESIS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RNA; SERRATIA;

ACRONICTA LEPORINA; ANABAENA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; SERRATIA; SERRATIA MARCESCENS;

EID: 0032478746     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(98)00279-8     Document Type: Article

References (32)

1. Miller, M.D. and Krause, K.L. (1996) Protein Sci. 5, 24-33.
2. Eaves, G.N. and Jeffries, C.D. (1963) J. Bacteriol. 85, 273-278.
3. Yonemura, K., Matsumoto, K. and Maeda, H. (1983) J. Biochem. Tokyo 93, 1287-1295.
4. Ball, T.K., Saurugger, P.N. and Benedik, M.J. (1987) Gene 57, 183-192.
5. Friedhoff, P., Kolmes, B., Gimadutdinow, O., Wende, W., Krause, K.L. and Pingoud, A. (1996) Nucleic Acids Res. 24, (14) 2632-2639.
6. Filimonova, M.N., Krause, K.L. and Benedik, M.J. (1994) Biochem. Mol. Biol. Int. 33, 1229-1236.
7. Meiss, G., Friedhoff, P., Hahn, M., Gimadutdinow, O. and Pingoud, A. (1995) Biochemistry 34, 11979-11988.
8. Nestle, M. and Roberts, W.K. (1969) J. Biol. Chem. 244, 5213-5218.
9. Nestle, M. and Roberts, W.K. (1969) J. Biol. Chem. 244, 5219-5225.
10. Biedermann, K., Jepsen, P.K., Riise, E. and Svendsen, I. (1989) Carlsberg Res. Commun. 54, 17-27.
11. Miller, M.D., Tanner, J., Alpaugh, M., Benedik, M.J. and Krause, K.L. (1994) Nature Struct. Biol. 1, 461-468.
12. Vincent, R.D., Hofmann, T.J. and Zassenhaus, H.P. (1988) Nucleic Acids Res. 16, 3297-3312.
13. Muro-Pastor, A.M., Flores, E., Herrero, A. and Wolk, C.P. (1992) Mol. Microbiol. 6, 3021-3030.
14. Ruiz-Carrillo, A. and Côté, J. (1993) Science 261, 765-769.
15. Friedhoff, P., Gimadutdinow, O., Rüter, T., Wende, W., Urbanke, C., Thole, H. and Pingoud, A. (1994) Prot. Expr. Purif. 5, 37-43.
16. Friedhoff, P., Meiss, G., Kolmes, B., Pieper, U., Gimadutdinow, O., Urbanke, C. and Pingoud, A. (1996) Eur. J. Biochem. 241, 572-580.
17. Kolmes, B., Franke, I., Friedhoff, P. and Pingoud, A. (1996) FEBS Lett. 397, 343-346.
18. Muro-Pastor, A.M., Flores, E., Herrero, A. and Flores, E. (1997) J. Mol. Biol. 268, 589-598.
19. Meiss, G., Franke, I., Gimadutdinow, O., Urbanke, C. and Pingoud, A. (1998) Eur. J. Biochem., in press.
20. Dake, E., Hofmann, T.J., McIntire, S., Hudson, A. and Zassenhaus, H.P. (1988) J. Biol. Chem. 263, 7691-7702.
21. Cöté, J., Renaud, J. and Ruiz-Carillo, A. (1989) J. Biol. Chem. 265, 3301-3310.
22. Chen, L.Y., Ho, H.C., Tsai, Y.C. and Liao, T.H. (1993) Arch. Biochem. Biophys. 303, 51-56.
23. Fraser, C.M., Casjens, S., Huang, W.M., Sutton, G.G., Clayton, R., Lathigra, R., White, O., Ketchum, K.A., Dodson, R., Hickey, E.K., Gwinn, M., Dougherty, B., Tomb, J.-F., Fleischmann, R.D., Richardson, D., Peterson, J., Kerlavage, A.R., Quackenbush, J., Salzberg, S., Hanson, M., van Vugt, R., Palmer, N., Adams, M.D., Gocayne, J., Weidmann, J., Utterback, T., Wat-they, L., McDonald, L., Artiach, P., Bowman, C., Garland, S., Fujii, C., Cotton, M.D., Horst, K., Roberts, K., Hatch, B., Smith, H.O. and Venter, J.C. (1997) Nature 390, 580-586.
24. Filimonova, M.N., Baratova, L.A., Vospelnikova, N.D., Zehltova, A.O. and Leschinskaia, I.B. (1981) Biokhimiia 46, 1660-1666.
25. Friedhoff, P., Gimadutinow, O. and Pingoud, A. (1994) Nucleic Acids Res. 22, 3280-3287.
26. Côté, J. and Ruiz-Carillo, A. (1993) Science 261, 765-769.
27. Lunin, V.Y., Levdikov, V.M., Shlyapnikow, S.V., Blagova, E.V., Lunin, V.V., Wilson, K.S. and Mikhailov, A.M. (1997) FEBS Lett. 412, 217-222.
28. Ito, W., Ishiguro, H. and Kurosawa, Y. (1991) Gene 102, 67-70.
29. Blum, E., Horst, G.B. and Kröger, M. (1994) J. Biochem. Biophys. Methods 29, 113-121.
30. Kindler, B. (1997) PhD thesis, Universität Hannover.
31. Laemmli, U.K. (1970) Nature 227, 680.
32. Jaenicke, R. (1987) Prog. Biophys. Mol. Biol. 49, 117-237.