Functional expression of GFP-tagged Kv1.3 and Kv1.4 channels in HEK 293 cells

European Journal of Neuroscience

Volumn 10, Issue 12, 1998, Pages 3908-3912

  Kupper, Jürgen ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Fusion protein; Green fluorescent protein; Recombinant K+ channel

Indexed keywords

CHIMERIC PROTEIN; GREEN FLUORESCENT PROTEIN; POTASSIUM CHANNEL; PROTEIN SUBUNIT; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; DEPOLARIZATION; EMBRYO; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

CELLS, CULTURED; ELECTROPHYSIOLOGY; GENE EXPRESSION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; INDICATORS AND REAGENTS; ION CHANNEL GATING; KIDNEY; KV1.3 POTASSIUM CHANNEL; KV1.4 POTASSIUM CHANNEL; LUMINESCENT PROTEINS; MEMBRANE POTENTIALS; POTASSIUM CHANNELS; POTASSIUM CHANNELS, VOLTAGE-GATED;

EID: 0032465106     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1460-9568.1998.00441.x     Document Type: Article

References (23)

1. Chandy, K.G. & Gutman, G.A. (1995) Voltage-gated potassium channel genes. In North, R.A. (eds) Handbook of Receptors and Channels: Ligand and Voltage-Gated Channels. CRC, Boca Raton, FL, pp. 1-71.
2. Cooper, E.C., Milroy, A., Jan, Y.N., Jan, L.Y. & Lowenstein, D.H. (1998) Presynaptic localization of Kv1.4-containing A-type potassium channels near excitatory synapses in the hippocampus. J. Neurosci., 18, 965-974.
3. Heinemann, S.H., Rettig, J., Graack, H.R. & Pongs, O. (1996) Functional characterization of Kv channel beta-subunits from rat brain. J. Physiol., 493, 625-633.
4. + channels by interaction with a family of membrane-associated guanylate kinases. Nature, 378, 85-88.
5. + channel clustering activity of PSD-95 and SAP97, two related membrane-associated putative guanylate kinases. Neuropharmacol., 35, 993-1000.
6. Kornau, H.C., Schenker, L.T., Kennedy, M.B. & Seeburg, P.H. (1995) Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. Science, 269, 1737-1740.
7. Ludin, B. & Matus, A. (1998) GFP illuminates the cytoskeleton. Trends Cell Biol., 8, 72-77.
8. + channel polypeptides in rat hippocampal neurons developing in situ and in vitro. J. Neurosci., 15, 3840-3851.
9. Marom, S. & Abbott, L.F. (1994) Modeling state-dependent inactivation of membrane currents. Biophys. J., 67, 515-520.
10. Marom, S., Goldstein, S.A., Kupper, J. & Levitan, I.B. (1993) Mechanism and modulation of inactivation of the Kv3 potassium channel. Receptors Channels, 1, 81-88.
11. Marom, S. & Levitan, I.B. (1994) State-dependent inactivation of the Kv3 potassium channel. Biophys. J., 67, 579-589.
12. Marshall, J., Molloy, R., Moss, G.W., Howe, J.R. & Hughes, T.E. (1995) The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function. Neuron, 14, 211-215.
13. + channels - more than model behavior. Trends Pharmacol. Sci., 18, 474-483.
14. 2+/calmodulin-dependent protein kinase. J. Neurosci., 17, 3379-3391.
15. Ruppersberg, J.P., Stocker, M., Pongs, O., Heinemann, S.H., Frank, R. & Koenen, M. (1991) Regulation of fast inactivation of cloned mammalian IK (A) channels by cysteine oxidation. Nature, 352, 711-714.
16. Scannevin, R.H. & Trimmer, J.S. (1997) Cytoplasmic domains of voltage-sensitive K+ channels involved in mediating protein-protein interactions. Biochem. Biophys. Res. Comms, 232, 585-589.
17. + channels in vivo. Prog. Brain Res., 105, 87-93.
18. Sheng, M. & Wyszynski, M. (1997) Ion-channel targeting in neurons Bioessays, 19, 847-853.
19. Songyang, Z., Fanning, A.S., Fu, C., Xu, J., Marfatia, S.M., Chishti, A.H., Crompton, A., Chan, A.C., Anderson, J.M. & Cantley, L.C. (1997) Recognition of unique carboxyl-terminal motifs by distinct PDZ domains. Science, 275, 73-77.
20. Stauber, R.H., Horie, K., Carney, P., Hudson, E.A., Tarasova, N.I., Gaitanaris, G.A. & Pavlakis, G.N. (1998) Development and applications of enhanced green fluorescent protein mutants. Biotechniques, 24, 462-471.
21. Swanson, R., Marshall, J., Smith, J.S., Williams, J.B., Boyle, M.B., Folander, K., Luneau, C.J., Antanavage, J., Oliva, C., Buhrow, S.A., Bennet, C., Stein, R.B. & Kaczmarek, L.K. (1990) Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain. Neuron, 4, 929-939.
22. Veh, R.W., Lichtinghagen, R., Sewing, S., Wunder, F., Grumbach, I.M. & Pongs, O. (1995) Immunohistochemical localization of five members of the Kv1 channel subunits: contrasting subcellular locations and neuron-specific co-localizations in rat brain. Eur. J. Neurosci., 7, 2189-2205.
23. Wei, A., Jegla, T. & Salkoff, L. (1996) Eight potassium channel families revealed by the C. elegans genome project. Neuropharmacol., 35, 805-829.