Identification of cpxR as a positive regulator essential for expression of the Shigella sonnei virF gene

Journal of Bacteriology

Volumn 180, Issue 14, 1998, Pages 3522-3528

  Nakayama, Shu Ichi ^a   Watanabe, Haruo ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PROTEIN CPXR; PROTEIN VIRF; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SHIGELLA SONNEI; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SHIGELLA; SHIGELLA SONNEI;

EID: 0032456890     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.14.3522-3528.1998     Document Type: Article

References (34)

1. Adler, B., C. Sasakawa, T. Tobe, S. Makino, K. Komatsu, and M. Yoshikawa. 1989. A dual transcriptional activation system for the 230kb plasmid genes coding for virulence-associated antigens of Shigella flexneri. Mol. Microbiol. 3:627-635.
2. Albright, L. M., E. Huala, and F. M. Ausubel. 1989. Prokaryotic signal transduction mediated by sensor and regulator protein pairs. Annu. Rev. Genet. 23:311-336.
3. Bernardini, M. L., A. Fontaine, and P. J. Sansonetti. 1990. The two-component regulatory system OmpR-EnvZ controls the virulence of Shigella flexneri. J. Bacteriol. 172:6274-6281.
4. Brosius, J., T. J. Dull, D. D. Sleeter, and H. F. Noller. 1981. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148:107-127.
5. Chang, A. C. Y., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
6. Danese, P. N., W. B. Snyder, C. L. Cosma, L. J. B. Davis, and T. J. Silhavy. 1995. The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP. Genes Dev. 9:387-398.
7. Di Rita, V. J., and J. J. Mekalanos. 1989. Genetic regulation of bacterial virulence. Annu. Rev. Genet. 23:455-482.
8. Dong, J., S. Iuchi, H.-S. Kwan, Z. Lue, and E. C. C. Lin. 1993. The deduced amino-acid sequence of the cloned cpxR gene suggests the protein is the cognate regulator for the membrane sensor, CpxA, in a two-component signal transduction system of Escherichia coli. Gene 136:227-230.
9. Gross, R., B. Arico, and R. Rappuoli. 1989. Families of bacterial signal-transducing proteins. Mol. Microbiol. 3:1661-1667.
10. Hale, T. L. 1991. Genetic basis of virulence in Shigella species. Microbiol. Rev. 55:206-224.
11. Johnston, T. C., R. B. Thompson, and T. O. Baldwin. 1986. Nucleotide sequence of the luxB gene of Vibrio harveyi and the complete amino acid sequence of the beta subunit of bacterial luciferase. J. Biol. Chem. 261:4805-4811.
12. Kato, J., K. Ito, A. Nakamura, and H. Watanabe. 1989. Cloning of regions required for contact hemolysis and entry into LLC-MK2 cells from Shigella sonnei form I plasmid: virF is a positive regulator gene for these phenotypes. Infect. Immun. 57:1391-1398.
13. Lukat, G. S., W. R. McClerary, A. M. Stock, and J. B. Stock. 1992. Phosphorylation of bacterial response regulator proteins by low molecular weight phosphodonors. Proc. Natl. Acad. Sci. USA 89:718-722.
14. Makino, S.-I., C. Sasakawa, K. Komatsu, T. Kurata, and M. Yoshikawa. 1986. A genetic determinant required for continuous reinfection of adjacent cells on a large plasmid in Shigella flexneri 2a. Cell 46:551-555.
15. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
16. Mekalanos, J. J. 1992. Environmental signals controlling expression of virulence determinants in bacteria. J. Bacteriol. 174:1-7.
17. Menard, R., P. J. Sansonetti, and C. Parsot. 1993. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J. Bacteriol. 175:5899-5906.
18. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Nakayama, S., S. Miyake, and H. Watanabe. Unpublished data.
20. Nakayama, S., and H. Watanabe. 1995. Involvement of cpxA, a sensor of a two-component regulatory system, in the pH-dependent regulation of expression of Shigella sonnei virF gene. J. Bacteriol. 177:5062-5069.
21. Ohmori, H., M. Saito, T. Yasuda, T. Nagata, T. Fujii, M. Wachi, and K. Nagai. 1995. The pcsA gene is identical to dinD in Escherichia coli. J. Bacteriol. 177:156-165.
22. Oka, A., H. Sugisaki, and M. Takanami. 1981. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147:217-226.
23. Parsot, C. 1994. Shigella flexneri: genetics of entry and intracellular dissemination in epithelial cells. Curr. Top. Microbiol. Immunol. 192:217-241.
24. Pogliano, J., A. S. Lynch, D. Belin, E. C. C. Lin, and J. Beckwith. 1997. Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system. Genes Dev. 11:1169-1182.
25. Rainwater, S., and P. M. Silverman. 1990. The Cpx proteins of Escherichia coli K-12: evidence that cpxA, ecfB, ssd, and eup mutations all identify the same gene. J. Bacteriol. 172:2456-2461.
26. Raivio, T. L., and T. J. Silhavy. 1997. Transduction of envelope stress in Escherichia coli by the Cpx two-component system. J. Bacteriol. 179:7724-7733.
27. Sakai, T., C. Sasakawa, and M. Yoshikawa. 1988. Expression of four virulence antigens of Shigella flexneri is positively regulated at the transcriptional level by the 30 kD virF protein. Mol. Microbiol. 2:589-597.
28. Sasakawa, C., J. M. Buysse, and H. Watanabe. 1992. The large virulence plasmid of Shigella. Curr. Top. Microbiol. Immunol. 180:21-44.
29. Stachel, S. E., G. An, C. Flores, and E. W. Nester. 1985. A Tn3-lacZ transposon for the random generation of β-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 4:891-898.
30. Stock, J. B., A. J. Ninfa, and A. M. Stock. 1989. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol. Rev. 53:450-490.
31. Takeshita, S., M. Sato, M. Toba, W. Masahashi, and T. Hashimoto-Gotoh. 1987. High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol-or-kanamycin-resistance selection. Gene 61:63-74.
32. Tobe, T., M. Yoshikawa, T. Mizuno, and C. Sasakawa. 1993. Transcriptional control of the invasion regulatory gene virB of Shigella flexneri: activation by VirF and repression by H-NS. J. Bacteriol. 175:6142-6149.
33. Venkatesan, M., J. M. Buysse, and D. J. Kopecko. 1988. Characterization of invasion plasmid antigen (ipaBCD) genes from Shigella flexneri: DNA sequence analysis and control of gene expression. Proc. Natl. Acad. Sci. USA 85:9317-9321.
34. Watanabe, H., E. Arakawa, K. Ito, J. Kato, and A. Nakamura. 1990. Genetic analysis of an invasion region by use of a Tn3-lac transposon and identification of a second positive regulator gene, invE, for cell invasion of Shigella sonnei: significant homology of InvE with ParB of plasmid P1. J. Bacteriol. 172:619-629.