Distinction between the influence of dielectric constant and of methanol concentration on trypsin-catalyzed hydrolysis and methanolysis

Journal of Microbiology and Biotechnology

Volumn 8, Issue 6, 1998, Pages 656-662

  Park, Hyun ^a   Chi, Young Min ^a  

^a Korea University   (South Korea)

Author keywords

Cryoenzymology; Dielectric constant; Hydrolysis; Methanolysis; Trypsin

Indexed keywords

METHANOL; TRYPSIN;

ARTICLE; BINDING AFFINITY; DIELECTRIC CONSTANT; ENZYME ANALYSIS; ENZYME MECHANISM; HYDROLYSIS; HYDROPHOBICITY; NONHUMAN; PEPTIDE SYNTHESIS;

FELIS CATUS;

EID: 0032427943     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Affleck, R., C. A. Haynes, and D. S. Clark. 1992. Solvent dielectric effects on protein dynamics. Proc. Natl. Acad. Sci. USA 89: 5167-5170.
2. Antonini, E. and P. Ascenzi. 1981. The mechanism of trypsin catalysis at low pH. J. Biol. Chem. 256: 12449-12455.
3. Bergmeyer, H. U. 1986. Methods of Enzymatic Analysis. 3rd ed. pp. 299-302. VCH Verlagsgesellschafe, Weinheim.
4. Cartwright, S. J. and S. G. Waley. 1987. Cryoenzymology of β-lactamases. Biochemistry 26: 5329-5337.
5. Compton, P. D., R. J. Coll, and A. L. Fink. 1986. Effect of methanol cryosolvents on the structural and catalytic properties of bovine trypsin. J. Biol. Chem. 261: 1248-1252.
6. Davis, M. E. and J. A. Mccammon. 1990. Electrostatics in biomolecular structure and dynamics. Chem. Rev. 91: 509-521.
7. Fersht, A. R. and J. E. Sternberg. 1989. Can a simple function for the dielectric response model electrostatic effect globular protein? Protein Eng. 2: 527-530.
8. α-Benzyloxycarbonyl-L-lysine p-nitrophenyl ester in dimethylsulfoxide at sub-zero temperatures. J. Biol. Chem. 249: 5027-5032.
9. Fink, A. L. and B. Painter. 1987. Characterization of unfolding of ribonuclease A in aqueous methanol solvents. Biochemistry 26: 1665-1671.
10. Haensler, M., N. Wehofsky, S. Gerisch, J.-D. Wissmann, and H.-D Jakubke. 1998. Reverse catalysis of elastase from porcine pancreas in frozen aqueous systems. Biol. Chem. 379: 71-74.
11. Hiromi, K. 1960. Theory of the influence of the dielectric constant on the rate of reaction in solution with application to enzyme reactions. I. Development of the theory and its application to some simple systems. Bull. Chem. Soc. Jpn. 33: 1251-1263.
12. Jönsson, Å., P. Adlercreutz, and B. Mattiasson. 1995. Effects of subzero temperatures on the kinetics of protease catalyzed dipeptide synthesis in organic media. Biotechnol. Bioeng. 46: 429-436.
13. Krishtalik, L. I., A. M. Kuznetsov, and E. L. Mertz. 1997. Electrostatics of proteins: Description in terms of two dielectric constants simultaneously. Proteins 28: 174-182.
14. Maurel, P. 1978. Relevance of dielectric constant and solvent hydrophobicity to the organic solvent effect in enzymology. J. Biol. Chem. 253: 1677-1683.
15. Mehler, E. L. and T. Solmajer. 1991. Electrostatic effects in proteins: Comparison of dielectric and charge models. Protein Eng. 4: 903-910.
16. Michels, P. C., J. S. Dordick, and D. S. Clark. 1997. Dipole formation and solvent electrostriction in subtilisin catalysis. J. Am. Chem. Soc. 119: 9331-9335.
17. Solmajer, T. and E. L. Mehler. 1991. Electrostatic screening in molecular dynamic simulation. Protein Eng. 4: 911-917.
18. Stevanato, R., B. Mondovi, O. Befani, M. Scarpa, and A. Rigo. 1994. Electrostatic control of oxidative deamination catalyzed by bovine serum amine oxidase. Biochem. J. 299: 317-320.
19. Stryer, L. 1995. Biochemistry, 4th ed. pp. 207-210. W. H. Freeman & Company, NY, U.S.A.
20. Tõugu, V., T. Tiivel, P. Talts, V. Šikšnis, S. Poyarkova, T. Kesvatera, and A. Aaviksaar. 1994. Electrostatic effects in trypsin reactions. Eur. J. Biochem. 222: 475-481.
21. Ullmann, G., M. Haensler, W. Gruender, M. Wagner, H. J. Hofmann, and H.-D. Jakubke. 1997. Influence of freeze-concentration effect on proteinase-catalyzed peptide synthesis in frozen aqueous systems. Biochim. Biophys. Acta 1338: 253-258.
22. Wlodek, S. T., J. Antosiewicz, and J. M. Briggs. 1997. On the mechanism of acetylcholinesterase action: The electrostatically induced acceleration of the catalytic acylation step. J. Am. Chem. Soc. 119: 8159-8165.
23. Zaks, A. and A. J. Russell. 1988. Enzymes in organic solvents: Properties and applications. J. Biotechnol. 8: 259-270.