Reverse catalysis of elastase from porcine pancreas in frozen aqueous systems

Biological Chemistry

Volumn 379, Issue 1, 1998, Pages 71-74

  Haensler, Marion ^a   Wehofsky, Nicole ^a   Gerisch, Sabine ^a   Wissmann, Johannes Dieter ^a   Jakubke, Hans Dieter ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Elastase; Frozen aqueous systems; Peptide synthesis

Indexed keywords

ELASTASE; PANCREATIC ELASTASE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME MECHANISM; ENZYME SPECIFICITY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY;

AMINO ACIDS; ANIMALS; CATALYSIS; ESTERS; FREEZING; PANCREATIC ELASTASE; PEPTIDES; SUBSTRATE SPECIFICITY; SWINE; TEMPERATURE;

SUIDAE;

EID: 0031961992     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Bizzozero, S.A., Dutler, H., Franzstack, R., and Rovagnati, B.A. (1985). Enzyme-catalysed peptide bond formation: Elastase-and δ-chymotrypsin-assisted synthesis of oligopeptides. Helv. Chim. Acta 68, 981-987.
2. Cerovsky, V., and Jost, K. (1985). Enzymatically catalysed peptide synthesis of oxytocin fragments 1-6 and 7-9. Coll. Czechoslovak. Chem. Commun. 50, 2775-2782.
3. α-unprotected peptide esters as acyl donors. J. Peptide Sci. 3, 93-98.
4. Haensler, M., and Jakubke, H.-D. (1996a). Nonconventional protease catalysis in frozen aqueous solutions. J. Peptide Sci. 2, 279-289.
5. Haensler, M., and Jakubke, H.-D. (1996b). Reverse action of hydrolases in frozen aqueous solutions. Amino Acids 11, 379-395.
6. Haensler, M., Ullmann, G., and Jakubke, H.-D. (1995). The application of papain, ficin and clostripain in kinetically controlled peptide synthesis in frozen aqueous solutions. J. Peptide Sci. 1, 283-287.
7. Haensler, M., Hahn, U., and Jakubke, H.-D. (1997). Reverse action of ribonuclease T1 in frozen aqueous systems. Biol. Chem. 378, 115-118.
8. Harper, J.W., Cook, R.R., Roberts, J., McLaughlin, B.J., and Powers, J.C. (1984). Active site mapping of the serine proteases human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell protease I and II, bovine chymotrypsin A and Staphylococcus aureus protease V8 using tripeptidethiobenzylester substrates. Biochemistry 23, 2995-3002.
9. Jakubke, H.-D., Eichhorn, U., Gerisch, S., Haensler, M., Salchert, K., Ullmann, G., and Ullmann, D. (1996a). Nonconventional approaches to enzyme-catalyzed peptide synthesis. In: Molecular Design and Bioorganic Catalysis, C.S. Wilcox and A.D. Hamilton, eds. (Deventer, The Netherlands: Kluwer Academic Publishers), pp. 53-70.
10. Jakubke, H.-D., Eichhorn, U., Haensler, M., and Ullmann, D. (1996b). Non-conventional enzyme catalysis: Application of proteases and zymogens in biotransformations. Biol. Chem. 377, 455-464.
11. Schechter, I., and Berger, A. (1967). On the size of the active site in proteinases. I. Papain. Biochem. Biophys. Res. Commun. 27, 157-162.
12. Schellenberger, V., Schellenberger, U., Mitin, Y., and Jakubke, H.-D. (1989). Characterization of the S′-subsite specificity of porcine pancreatic elastase. Eur. J. Biochem. 179, 161-163.
13. Schellenberger, V., Schuster, M., and Jakubke, H.-D. (1990). Optimization of protease-catalysed acyl transfer reactions. Determination of the partition constant characterizing nucleophile efficiency. Biocatalysis 4, 105-111.
14. Schuster, M., Aaviksaar, A., Haga, M., Ullmann, U., and Jakubke, H.-D. (1991). Protease-catalysed peptide synthesis in frozen aqueous systems: The "freeze-concentration model". Biomed. Biochim. Acta 50, 84-89.
15. Schuster, M., Ullmann, G., and Jakubke, H.-D. (1993a). Chymotrypsin-catalysed peptide synthesis in ice: Use of unprotected amino acids as acyl acceptors. Tetrahedron Lett. 34, 5701-5702.
16. Schuster, M., Jakubke, H.-D., and Aaviksaar, A. (1993b). Thermodynamically controlled chymotrypsin-catalysed peptide synthesis in frozen aqueous solutions. In: Chemistry of Peptides and Proteins, Vols 5/6, DWI Reports, Vols 112 A/B, P. Brandenburg, V.T. Ivanow, and W. Voelter, eds. (Aachen; Germany; Verlag Mainz, Wissenschaftsverlag), pp. 203-209.
17. Strambini, G.B., and Gabillieri, E. (1996). Proteins in frozen solutions: Evidence of ice-induced partial unfolding. Biophys. J. 70, 971-976.
18. 2 subsites of elastase. Eur. J. Biochem. 102, 111-116.
19. Tougu, V., Meos, H., Haga, M., Aaviksaar, A., and Jakubke, H.-D. (1993). Peptide synthesis by chymotrypsin in frozen solutions. FEBS Lett. 329, 40-42.
20. Ullmann, G., Haensler, M., Gruender, W., Wagner, M., Hofmann, H.-J., and Jakubke, H.-D. (1997). Influence of freeze-concentration effect on proteinase-catalysed peptide synthesis in frozen aqueous systems. Biochim. Biophys. Acta 1338, 253-258.
21. William-Smith, D.L., Bray, R.C., Barber, M.J., Tsopanakis, A.D., and Vincent, S.P. (1977). Changes in apparent pH on freezing aqueous buffer solutions and their relevance to biochemical electron-paramagnetic-resonance spectroscopy. Biochem. J. 167, 593-600.