Detection of GB virus C RNA by GBV-C LCx and two PCR assays with primers from the 5' non-coding and NS5B region

Journal of Virological Methods

Volumn 76, Issue 1-2, 1998, Pages 43-49

  Berg, E S ^a   Jarvis, L M ^b   Simmonds, P ^b   Bell, H ^c   Skaug, K ^a  

^a NORWEGIAN INSTITUTE OF PUBLIC HEALTH   (Norway)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c AKER UNIVERSITY HOSPITAL   (Norway)

Author keywords

GB virus C hepatitis G virus; Nucleic acid detection; Polymerase chain reaction

Indexed keywords

PRIMER RNA; VIRUS RNA;

ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; GENE AMPLIFICATION; HEPATITIS; HEPATITIS G VIRUS; HUMAN; MAJOR CLINICAL STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS GENOME;

5' UNTRANSLATED REGIONS; DNA PRIMERS; FLAVIVIRIDAE; GENOME, VIRAL; HEPATITIS C, CHRONIC; HEPATITIS, VIRAL, HUMAN; HUMANS; REAGENT KITS, DIAGNOSTIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS;

GB VIRUS C/HEPATITIS G VIRUS; HEPATITIS C VIRUS;

EID: 0032403702     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(98)00122-0     Document Type: Article

References (16)

1. Alter H.J., Nakatsuji Y., Melpolder J., Wages J., Wesley R., Shih J.W.-K., Kim J.P. The incidence of transfusion-associated hepatitis G virus infection and its relation to liver disease. New Engl. J. Med. 336:1997;747-754.
2. Bell H., Hellum K., Harthug S. et al. Genotype viral load and age as independent predictors of treatment outcome of interferon-(2a treatment in patients with chronic hepatitis C. Scand. J. Infect. Dis. 29:1997;17-22.
3. Bretagne S., Costa J.M., Vidaud M., Tran J., Nhieu V., Fleury-Feith J. Detection of Toxoplasma gondii by competitive DNA amplification of bronchoalveolar lavage samples. J. Infect. Dis. 168:1993;1585-1588.
4. Dille B.J, Surowy T.K., Gutierrez R.A. et al. An ELISA for detection of antibodies to the E2 protein of GB virus C. J. Infect. Dis. 175:1997;458-461.
5. Jarvis L.M., Davidson F., Hanley J.P., Yap P.L., Ludlam C.A., Simmonds P. Infection with hepatitis G virus among recipients of plasma products. Lancet. 348:1996;1352-1355.
6. Kao J.H., Chen P.J., Chen W., Hsiang S.C., Lai M.Y., Chen D.S. Amplification of GB virus-C/hepatitis G virus RNA with primers from different regions of the viral genome. J. Med. Microbiol. 51:1997;284-289.
7. Kwok S., Kellogg D.E., McKinney N., Spasic D., Goda L., Levenson C., Sninsky J.J. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res. 18:1990;999-1005.
8. Leary T.P., Muerhoff A.S., Simons J.N. et al. Sequence and genomic organization of GBV-C: a novel member of the flaviviridae associated with human non-A-E hepatitis. J. Med. Virol. 48:1996;60-67.
9. Leary T.P., Muerhoff A.S., Simons J.N. et al. Consensus oligonucleotide primers for the detection of GB virus C in human cryptogenic hepatitis. J. Virol. Methods. 56:1996;119-121.
10. Linnen J., Wages J. Jr., Zhang-Keck Z.Y. et al. Molecular cloning and disease association of hepatitis G virus: a transfusion-transmissible agent. Science. 271:1996;505-508.
11. Muerhoff A.S., Simons J.N., Erker J.C., Desai S.M., Mushahwar I.K. Identification of conserved nucleotide sequences within the GB virus C 5′-untranslated region: design of PCR primers for detection of viral RNA. J. Virol. Methods. 62:1996;55-62.
12. Simons J.N., Leary T.P., Dawson G.J., Pilot-Matias T.J., Muerhoff A.S., Schlauder G.G., Desai S.M., Mushahwar I.K. Isolation of novel virus-like sequences associated with human hepatitis. Nat. Med. 1:1995;564-569.
13. Tacke M., Kiyosawa K., Stark K., Schlueter V., Ofenloch-Haehnle B., Hess G., Engel A.M. Detection of antibodies to a putative hepatitis G virus envelope protein. Lancet. 349:1997;318-320.
14. Tacke M., Schmolke S., Schlueter V., Sauleda S., Esteban J.I., Tanaka E., Kiyosawa K., Alter H.J., Schmitt U., Hess G., Ofenloch-Haehnle B., Engel A.M. Humoral immune response to the E2 protein of hepatitis G virus is associated with long-term recovery from infection and reveals a high frequency of hepatitus G virus exposure among healthy blood donors. Hepatology. 78:1997;1626-1633.
15. Yoshiba M., Okamoto H., Mishiro S. Detection of the GBV-C hepatitis virus genome in serum from patients with fulminant hepatitis of unknown aetiology. Lancet. 346:1995;1131-1132.
16. Zuckerman A.J. Alphabet of hepatitis viruses. Lancet. 347:1996;558-559.