Solid phase ELISA for determination of the virus dose dependant sensitivity of human cytomegalovirus to antiviral drugs in vitro

Antiviral Research

Volumn 40, Issue 1-2, 1998, Pages 105-112

  Ljungdahl Ståhle, Ewa ^a   Schloss, Lottie ^a   Sundqvist, Vivi Anne ^b   Brytting, Maria ^a   Hökeberg, Ingegerd ^c   Cox, Susan ^a   Wahren, Britta ^a   Linde, Annika ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c UPPSALA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Antiviral drugs; Antiviral sensitivity assay; Cytomegalovirus; ELISA

Indexed keywords

ANTIVIRUS AGENT; FOSCARNET; GANCICLOVIR;

ANTIVIRAL ACTIVITY; ARTICLE; CONTROLLED STUDY; DRUG SENSITIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; INTERMETHOD COMPARISON; PRIORITY JOURNAL; VIROGENESIS; VIRUS CULTURE; VIRUS REPLICATION; VIRUS STRAIN;

ANTIVIRAL AGENTS; CELL LINE; CYTOMEGALOVIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; PLAQUE ASSAY; REPRODUCIBILITY OF RESULTS; VIRUS REPLICATION;

EID: 0032400901     PISSN: 01663542     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-3542(98)00054-0     Document Type: Article

References (17)

1. Baldanti F., Underwood M.R., Stanat S.C. et al. Single amino acids changes in the DNA polymerase confer foscarnet resistance and slow-growth phenotype, while mutations in the UL97-encoded phosphotransferase confer ganciclovir resistance in three double-resistant human cytomegalovirus strains recovered from patients with AIDS. J. Virol. 70(3):1996;1390-1395.
2. Chou S., Guentzel S., Michels K.R., Miner R.C., Drew W.L. Frequency of UL97 phosphotransferase mutations related to ganciclovir resistance in clinical cytomegalovirus isolates. J. Infect. Dis. 172:1995;239-242.
3. Dankner W.M., Scholl D., Stanat S.C., Martin M., Sonke R.L., Spector S.A. Rapid antiviral DNA-DNA hybridization assay for human cytomegalovirus. J. Virol. Methods. 28:1990;293-298.
4. Drew W.L., Miner R.C., Busch D.F. et al. Cytomegalovirus resistance to antiviral therapies. Am. J. Health-System Pharmacol. 53(2):1996;17-23.
5. Drew W.L., Miner R., Saleh E. Antiviral susceptibility testing of cytomegalovirus: criteria for detecting resistance to antivirals. Clin. Diagn. Virol. 1:1993;179-185.
6. Erice A., Gil-Roda C., Pérez J.L. et al. Antiviral susceptibilities and analysis of UL97 and DNA polymerase sequences of clinical cytomegalovirus isolates from immunocompromised patients. J. Infect. Dis. 175:1997;1087-1092.
7. Field A.K., Biron K.K. The end of innocence revisited: resistance of herpesviruses to antiviral drugs. Clin. Microbiol. Rev. 7(1):1994;1-13.
8. Gadler H. Nucleic acid hybridization for measurements of effects of antiviral compounds on human cytomegalovirus DNA replication. Antimicrob. Agents. Chemother. 24(3):1983;370-374.
9. Gao W.Y., Johns D.G., Chokekijchai S., Mitsuya H. Disparate actions of hydroxyurea in potentiation of purine and pyrimidine 2′-3′-dideoxynucleoside activities against replication of human immunodeficiency virus. Proc. Natl. Acad. Sci. 92:1995;8333-8337.
10. Gerna G., Sarasini A., Percivalle E., Zavattoni M., Baldanti F., Revello M.G. Rapid screening for resistance to ganciclovir and foscarnet of primary isolates of human cytomegalovirus from culture-positive blood samples. J. Clin. Microbiol. 33(3):1995;738-741.
11. Harmenberg J., Wahren B., Öberg B. Influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine. Intervirology. 14:1980;239-244.
12. Lipson S.M., Tseng L.F., Kaplan M.H., Biondo F.X. Antiviral susceptibility testing of cytomegalovirus from primary culture using shell vial assay to detect the late viral antigen. Diagn. Microbiol. Infect. Dis. 17:1993;283-291.
13. Palmer S., Rasmussen H., Harmenberg J., Cox S. Intracellular metabolism of 3′-azido-3′-deoxythymidine in the presence of ganciclovir or foscarnet. Antiviral Chem. Chemother. 7(1):1996;14-20.
14. Smith I.L., Cherrington J.M., Jiles R.E., Fuller M.D., Freeman W.R., Spector S.A High-level resistance of cytomegalovirus to ganciclovir is associated with alterations in both the UL97 and DNA polymerase genes. J. Inf. Dis. 176:1997;69-77.
15. Sullivan V., Talarico C.L., Stanat S.C., Davis M., Coen D.M., Biron K.K. A protein kinase homologue controls phosphorylation of ganciclovir in human cytomegalovirus-infected cells. Nature. 358:1992;162-164.
16. Tatarowics W.A., Lurain N.S., Thompson K.D. In situ ELISA for the evaluation of antiviral compounds effective against human cytomegalovirus. J. Virol. Methods. 35:1991;207-215.
17. Wahren B., Harmenberg J., Sundqvist V.A., Levén B., Sköldenberg B. A novel method for determining the sensitivity of herpes simplex virus to antiviral compounds. J. Virol. Methods. 6:1983;141-149.