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For an example of protein-DNA interaction see Thunnisen, A.-M., Dijkstra, W. H. A. J., Kalk, K. H., Rozeboom, H. J., Engel, H., Keck, W. and Dijkstra, B. W. (1994). Nature, 367, 750. For a recent review see Klug, A. and Choo, Y. (1997). Curr. Opn. Str. Biol., 7, 117.
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9-N-butyladenine was used for this purpose and was prepared according to a literature procedure. See Nowick, J. S., Chen, J. S. and Noronha, G. (1993). J. Am. Chem. Soc., 115, 7636.
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Nowick, J.S.1
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32
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0013596139
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The input conformations were kept as close to the binding geometry as possible
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PCMODEL for Windows, version 5.13. The input conformations were kept as close to the binding geometry as possible.
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PCMODEL for Windows, Version 5.13
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34
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85034282992
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note
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10C, 70.06; H, 7.10.
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35
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85034277470
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note
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3 is only 1.64 mM at 27°C. However, we were able to solubilise as much as 6 mM of host 4 in the presence of the guest.
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36
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85034300756
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note
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The acid was initially dissolved in THF, required volumes added into the NMR tubes and evaporated to dryness in vacuo for 3h.
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