Further study on the two pivotal parts of hlg2 for the full hemolytic activity of staphylococcal γ-hemolysin

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 9, 1998, Pages 1745-1750

  Yokota, Kenji ^a   Sugawara, Noriko ^a   Nariya, Hirofumi ^a   Kaneko, Jun ^a   Tomita, Toshio ^a   Kamio, Yoshiyuki ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Bi component cytotoxin; Hlg2; LukS; Staphylococcal hemolysin

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; GAMMA HEMOLYSIN, STAPHYLOCOCCUS AUREUS; GAMMA-HEMOLYSIN, STAPHYLOCOCCUS AUREUS; HEMOLYSIN; LEUKOCIDIN; LUKF PROTEIN, STAPHYLOCOCCUS AUREUS;

ANIMAL; ARTICLE; CELL MEMBRANE; ERYTHROCYTE; HEMOLYSIS; HUMAN; METABOLISM; RABBIT; STAPHYLOCOCCUS AUREUS;

ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL MEMBRANE; ERYTHROCYTES; HEMOLYSIN PROTEINS; HEMOLYSIS; HUMANS; LEUKOCIDINS; RABBITS; STAPHYLOCOCCUS AUREUS;

EID: 0032153407     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.1745     Document Type: Article

References (15)

1. Tomita, T. and Kamio, Y., Molecular biology of the pore-forming cytolysins from Staphylococcus aureus, α- and γ-hemolysin and leukocidin. Biosci. Biotechnol. Biochem., 61, 565-572 (1997).
2. Sugawara, N., Tomita, T., and Kamio, Y., Assembly of Staphylococcus aureus y-hemolysin into a pore-forming ring-shaped complex on the surface of human erythrocytes. FEBS Lett., 410, 333-337 (1997).
3. Kamio, Y., Rahman, A., Nariya, H., Ozawa, T., and Izaki, K., The two staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, share one component in common. FEBS Lett., 321, 15-18 (1993).
4. Rahman, A., Izaki, K., and Kamio, Y., Gamma-hemolysin genes in the same family with lukF and lukS genes in methicillin resistant Staphylococcus aureus. Biosci. Biotechnol. Biochem., 57, 1234-1236 (1993).
5. Nariya, H. and Kamio, Y., Identification of the minimum segment essential for the Hyll-specific function of staphylococcal y-hemolysin. Biosci. Biotechnol. Biochem., 61, 1786-1788 (1997).
6. Nariya, H. and Kamio, Y., Identification of the essential regions for LukS- and Hyll-specific function of staphylococcal leukocidin and y-hemolysin. Biosci. Biotechnol. Biochem., 59, 1603-1604 (1995).
7. Sudo, K., Choorit, W., Asami, I., Kaneko, J., Muramoto, K., and Kamio, Y., Substitution of lysine for arginine in the TV-temi-nal 217th amino acid residue of the Hyll of staphylococcal y-hemolysin lowers the activity of the toxin. Biosci. Biotechnol. Biochem., 59, 1786-1789 (1995).
8. Horton, R., Hunt, H., Ho, S., Pullen, J., and Pease, L., Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene, 11, 66-68 (1989).
9. Nariya, H., Asami, I., Ozawa, T., Beppu, Y., Izaki, K., and Kamio, Y., Improved method for purification of leukocidin and gamma-hemolysin components from Staphylococcus aureus. Biosci. Biotechnol. Biochem., 57, 2198-2199 (1993).
10. Ozawa, T., Kaneko, J., and Kamio, Y., Essential binding of LukF of staphylococcal y-hemolysin followed by the binding of Hyll for the hemolysis of human erythrocytes. Biosci. Biotechnol. Biochem., 59, 1181-1183 (1995).
11. Kaneko, J., Ozawa, T., Tomita, T., and Kamio, Y., Sequential binding of staphylococcal y-hemolysin to human erythrocytes and complex formation of the hemolysin on the cell surface. Biosci. Biotechnol. Biochem., 61, 846-851 (1997).
12. Scherrer, R. and Gerhardt, P., Molecular sieving by the Bacillus megaterium cell wall and protoplast. J. Bacteriol., 107, 718-735 (1971).
13. Sabirov, R. Z., Krasilnikov, O. V., Ternovsky, V. I., and Merziliak, P. G., Relation between ionic channel conductance and conductivity of media containing different nonelectrolytes. Gen. Physiol. Biophys., 12, 95-111 (1993).
14. Song, L., Hobaugh, M. R., Shustak, C., Cheley, S, Bayley, H., and Gouaux, J. E., Structure of staphylococcal a-hemolysin, a heptameric transmembrane pore. Science, 274,1859-1866(1997).
15. Gouaux, J. E., Hobaugh, M., and Song, L., alpha-Hemolysin, gamma-hemolysin, and leukocidin from Staphylococcus aureus: distant in sequence but similar in structure. Protein Sci., 6, 2631-2635 (1997).