A highly sensitive whole blood natural killer cell assay

Journal of Immunological Methods

Volumn 217, Issue 1-2, 1998, Pages 177-184

  Bromelow, K V ^a   Galea Lauri, J ^a   O'Brien, M E ^b,c   Souberbielle, B E ^a,b  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b ROYAL MARSDEN HOSPITAL   (United Kingdom)

^c MAIDSTONE HOSPITAL   (United Kingdom)

Author keywords

K562 erythroleukaemic cells; Natural killer cell assay; NK cells; Whole blood

Indexed keywords

BIOMATERIAL; CD14 ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD56 ANTIGEN; CHROMIUM; EDETIC ACID; GLUCOSE; HEPARIN;

ARTICLE; ASSAY; BLOOD SAMPLING; CELL LABELING; CELL POPULATION; CELL STRAIN K 562; CYTOLYSIS; EFFECTOR CELL; HUMAN; HUMAN CELL; INCUBATION TIME; INTERMETHOD COMPARISON; LEUKEMIA CELL LINE; MONONUCLEAR CELL; NATURAL KILLER CELL; PRIORITY JOURNAL; TARGET CELL;

ANTICOAGULANTS; ANTIGENS, CD56; CELL SEPARATION; CITRIC ACID; CYTOTOXICITY TESTS, IMMUNOLOGIC; EDETIC ACID; FLOW CYTOMETRY; GLUCOSE; HEPARIN; HUMANS; K562 CELLS; KILLER CELLS, NATURAL; LYMPHOCYTE DEPLETION; LYMPHOCYTE SUBSETS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 0032144257     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00109-4     Document Type: Article

References (23)

1. Bancroft G.J. The role of natural killer cells in innate resistance to infection. Curr. Opin. Immunol. 5:1993;503-510.
2. Bloom E.T., Babitt J.T. Monocyte-mediated augmentation of human natural cell-mediated cytotoxicity. Cell. Immunol. 91:1985;21-32.
3. Bloom E.T., Babitt J.T., Kawakami K. Monocyte-mediated augmentation of human natural cell-mediated cytotoxicity: conditions, monocyte and effector cell characteristics. J. Immunol. 137:1986;172-178.
4. Bocchieri M.H., Talle M.A., Maltese L.M., Ragucci I.R., Hwang C.C., Goldstein G. Whole blood culture for measuring mitogen induced T cell proliferation provided superior correlation's with disease state and T cell phenotype in asymptomatic HIV-infected subjects. J. Immunol. Meth. 181:1995;3-243.
5. Bruunsgaard H., Pedersen C., Skinhoj P., Perdersen B.K. Clinical progression of HIV infection: role of NK cells. Scand. J. Immunol. 46:1997;91-95.
6. Chang Z.L., Whiteside T.L., Heberman R.B. Immunoregulatory role of in vitro differentiated macrophages on human killer (NK)-cell activity. Cell. Immunol. 125:1990;83-96.
7. Chang L., Gusewitch G.A., Chritton D.B., Folz J.C., Lebeck L.K., Nehlsen-Cannarella S.L. Rapid flow cytometric assay for the assessment of natural killer cell activity. J. Immunol. Meth. 166:1993;45-54.
8. Fletcher M.A., Baron G.C., Ashman M.R., Fischl M.A., Klimas N.G. Use of whole blood methods in assessment of immune parameters in immunodeficiency states. Diagn. Clin. Immunol. 5:1987;69-81.
9. Gaines H., Anderson L., Biberfield G. A new method for measuring lymphoproliferation at the single-cell level in whole blood cultures by flow cytometry. J. Immunol. Meth. 195:1996;63-72.
10. Hatam L., Shuval S., Bonagura V.R. Flow cytometric analysis of natural killer cell function as a clinical assay. Cytometry. 16:1994;59-68.
11. Jason J., Larned J. Single-cell cytokine profiles in normal humans: comparison of flow cytometric reagents and stimulation protocols. J. Immunol. Meth. 207:1997;13-22.
12. Morales A., Ottenhof P.C. Clinical application of a whole blood assay for human natural killer (NK) cell activity. Cancer. 52:1983;667-670.
13. Ortaldo J.R., Oldham R.K., Cannon G.C., Heberman R.B. Specificity of natural cytotoxic reactivity of normal lymphocytes against a myeloid leukaemia cell line. J. Natl. Cancer Inst. 59:1977;77-82.
14. Papadopoulos N.G., Dedoussis G.V., Spanakos A.D., Gritzapis A.D., Baxevanis C.N., Papamichail M. An improved fluorescence assay for the determination of lymphocyte-mediated cytotoxicity using flow cytometry. J. Immunol. Meth. 177:1994;101-111.
15. Pizzolo G., Trentin L., Vinante F.et al. Natural killer cell function and lymphoid subpopulations in acute non-lymphoblastic leukaemia in complete remission. Br. J. Cancer. 58:1988;368-372.
16. Rees R.C., Platts A.A. A modified short-term cytotoxicity test: assessment of natural cell-mediated cytotoxicity. J. Immunol. Meth. 62:1983;79-85.
17. 51Cr release assay for measurement of natural killer activity in HIV-infected patients AIDS. 10:1996;676-678.
18. Van-den-Bosch G., Preijers F., Vreugdenhil A., Hendriks J., Maas F., De-Witte T. Granulocyte-macrophage colony-stimulating factor (GM-CSF) counteracts the inhibiting effect of monocytes on natural killer (NK) cells. Clin. Exp. Immunol. 101:1995;515-520.
19. Vujanovick N.L., Nagashama S., Herberman R.B., Whiteside T.L. Nonsecretory apoptotic killing by human NK cells. J. Immunol. 157:1996;1117-1126.
20. Weir R.E., Morgan A.R., Britton W.J., Butlin C.R., Dockrell H.M. Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity. J. Immunol. Meth. 176:1994;93-101.
21. Whiteside T.L., Heberman R.B. The role of natural killer cells in human disease. Clin. Immunol. Immunopathol. 53:1989;1-23.
22. Whiteside T.L., Heberman R.B. The role of natural killer cells in immunosurveillance of cancer. Curr. Opin. Immunol. 53:1995;1-23.
23. Whiteside, T.L., Heberman, R.B., 1995b. Measurements of natural killer cell numbers and function in human. In: Methods in Neurosciences, Academic Press, 24, 1995, pp. 10-23.