FLT3: Receptor and ligand. Biology and potential clinical application

Cytokine and Growth Factor Reviews

Volumn 9, Issue 1, 1998, Pages 37-48

  Shurin, Michael R ^a   Esche, Clemens ^b   Lotze, Michael T ^b  

^a UNIVERSITY OF PITTSBURGH CANCER INSTITUTE   (United States)

^b NONE

Author keywords

Dendritic cells; FL; FLT3; Growth factors; Hematopoiesis

Indexed keywords

CYTOKINE; FETAL LIVER KINASE-2; FLT3 LIGAND PROTEIN; FMS LIKE TYROSINE KINASE 3; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 11; INTERLEUKIN 12; INTERLEUKIN 3; INTERLEUKIN 6; LIGAND; MEMBRANE PROTEIN; ONCOPROTEIN; PROTEIN TYROSINE KINASE; UNCLASSIFIED DRUG;

ANIMAL; CELL DIFFERENTIATION; CELL PROLIFERATION; CYTOKINE PRODUCTION; GENE MAPPING; GENE STRUCTURE; GENETICS; HEMATOPOIESIS; HUMAN; IMMUNOTHERAPY; PHYSIOLOGY; PROTEIN STRUCTURE; REVIEW;

ANIMALIA; MURINAE;

EID: 0032030339     PISSN: 13596101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1359-6101(97)00035-X     Document Type: Article

References (103)

1. Civin CI, Gore SD. Antigenic analysis of hematopoiesis: A review. J Hematother 1993, 2, 137-144.
2. Kriegler AB, Verschoor SM, Bernardo D, Bertoncello I. The relationship between different high proliferative potential colony-forming cells in the mouse bone marrow. Exp Hematol 1994, 22, 432-440.
3. Issaad C, Croisille L, Katz A, Vainchenker W, Coulombel L. A murine stromal cell line allows the proliferation of very primitive human CD34++/CD38- progenitor cells in long-term cultures and semisolid assays. Blood 1993, 81, 2916-2924.
4. Muench MO, Cupp J, Polakoff J, Roncarolo MG. Expression of CD33, CD38 and HLA-DR on CD34+ human fetal liver progenitors with a high proliferative potential. Blood 1994, 83, 3170-3181.
5. Ogawa M. Differentiation and proliferation of hematopoietic stem cells. Blood 1993, 81, 2844-2853.
6. Galy A, Travis M, Cen D, Chen B. Human TB, Natural Killer, and Dendritic Cells arise from a common bone marrow progenitor cell subset. Immunity 1995, 3, 459-471.
7. Metcalf D. Hematopoietic regulators: Redundancy or subtlety? Blood 1993, 82, 3515-3523.
8. Ullrich A, Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell 1990, 61, 203-212.
9. Shibuya M, Yamaguchi S, Yamane A, Ikeda T, Tojo A, Matsushime H, Sato M. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flk) closely related to the fms family. Oncogene 1990, 5, 519-524.
10. Matthews W, Jordan CT, Gavin M, Jerkins NA, Copeland NG, Lemischka IR. A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit. Proc Natl Acad Sci USA 1991, 88, 9026-9030.
11. Matthews W, Jordan CT, Wiegand GW, Pardoll D, Lemischka IR. A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell 1991, 65, 1143-1152.
12. Rosnet O, Marchetto S, de Lapeyriere O, Birnbuam D. Murine Flt3, a gene encoding a novel tyrosine kinase receptor of the PDGFR/CSF1R family. Oncogene 1991, 6, 1641-1650.
13. Jaye M, Schlessinger J, Dionne C. Fibroblast growth factor receptor tyrosine kinases: Molecular analysis and signal transduction. Biochem Biophys Acta 1992, 1135, 185-199.
14. Galland F, Karamysheva A, Pebusque MJ, Borg JP, Rottapel R, Dubreuil P, Rosnet O, Birnbaum D. The FLT4 gene encodes a transmembrane tyrosine kinase related to the VEGF receptor. Oncogene 1993, 8, 1233-1240.
15. Waltenberger J, Claesson-Welsh L, Siegbahn A, Shibuya M, Heldin CH. Different signal transduction properties of KDR and FLT1, two receptors for vascular endothelial growth factor. J Biol Chem 1994, 269, 26988-26995.
16. Rosnet O, Mattei MG, Marchetto S, Birnbaum D. Isolation and chromosomal localization of a novel FMS-like tyrosine kinase gene. Genomics 1991, 9, 380-385.
17. Lyman SD, James L, Zappone J, Sleath PR, Beckmann MP, Bird T. Characterization of the protein encoded by flt3 (flk2) receptor-like tyrosine kinase gene. Oncogene 1993, 8, 815-822.
18. Small D, Levenstein M, Kim E, Carow C, Amin S, Rockwell P, Witte L, Burrow C, Ratajczak MZ, Gewirtz AM, Civin CL. STK-1, the human homolog of Flk-2/Flt-3, is selectively expressed in CD34- human bone marrow cells and is involved in the proliferation of early progenitor/stem cells. Proc Natl Acad Sci USA 1994, 91, 459-463.
19. Iwama A, Okano K, Sudo T, Matsuda Y, Suda T. Molecular cloning of a novel receptor tyrosine kinase gene STK, derived from enriched hematopoietic stem cells. Blood 1994, 83, 3160-3169.
20. Agnes F. et al. Genomic structure of the downstream part of the human FLT3 gene: exon/intron structure conservation among genes encoding receptor tyrosine kinase (RTK) of subclass III. Gene 1994, 145, 283-288.
21. Sherr C. Colony-stimulating factor-1 receptor. Blood 1990, 75, 1-12.
22. Williams D, de Vries P, Namen A, Widmer M, Lyman S. The steel factor. Dev Biol 1992, 151, 368-376.
23. Stanley ER, Guilbert LJ, Tushinski RJ, Bartelmez SH. CSF-1 - A mononuclear phagocyte lineage-specific hemopoietic growth factor. J Cell Biochem 1983, 21, 151-159.
24. Sacca R, Stanley ER, Sherr CJ, Rettenmier CW. Specific binding of the mononuclear phagocyte colony-stimulating factor CSF-1 to the product of the v-fms oncogene. Proc Natl Acad Sci USA 1986, 83, 3331-3335.
25. McNiece IK, Langley KE, Zsebo KM. The role of recombinant stem cell factor in early B cell development. J Immunol 1991, 146, 3785-3790.
26. Motro B, Van Der Kooy D, Rossant J, Reith A, Bernstein A. Contiguous patterns of c-kit and Steel expression: Analysis of mutation at the W and SI loci. Development 1991, 113, 1207-1221.
27. Tsuji K, Zsebo KM, Ogawa M. Enhancement of murine blast cell colony formation in culture by recombinant rat stem factor ligand for c-kit. Blood 1991, 78, 1223-1229.
28. Godfrey DI, Zlotnik A, Suda T. Phenotypic and functional characterization of c-kit expression during intrathymic T cell development. J Immunol 1992, 149, 2281- 2285.
29. Lyman SD, James L, Vanden BT, de Vries P, Brasel K, Gliniak B, Hollingsworth LT, Picha KS, McKenna HJ, Splett RR, Fletcher FA, Maraskovsky E, Farrah T, Foxworthe D, Williams DE, Beckmann MP. Molecular cloning of a ligand for the flt3/flk2 tyrosine kinase receptor: A proliferative factor for primitive hematopoietic cells. Cell 1993, 75, 1157-1167.
30. Lyman SD, Brasel K, Rousseau A-M, Williams DE. The flt3 ligand: a hematopoietic stem cell factor whose activities are distinct from steel factor. Stem Cells 1994, 12, 99-107.
31. Lyman SD, James L, Johnson L, Brasel K, de Vries P, Escobar SS, Downey H, Splett RR, Beckmann MP, McKenna HJ. Cloning of the human homologue of the murine flt3 ligand: A growth factor for early hematopoietic progenitor cells. Blood 1994, 83, 2795-2801.
32. Hannum C, Culpepper J, Campbell D, McClanahan T, Zurawski S, Bazan JF, Kastelein R, Hudak S, Wagner J, Mattson H, Luh J, Duda G, Martina N, Peterson D, Manon S, Shanafelt A, Muench M, Kelner G, Namikawa R, Rennick D, Roncarolo M-G, Zlotnic A, Rosnet O, Dubreuil P, Birnbaum D, Lee F. Ligand for flt3/flk2 receptor regulates growth of hematopoietic stem cells and is encoded by variant RNAs. Nature 1994, 68, 643-648.
33. Maroc N, Rottapel R, Rosnet O, Marchetto, S, Lavezzi C, Mannoni P, Birnbaum D, Dubreuil P. Biochemical characterization and analysis of the transforming potential of the FLT3/FLK2 receptor tyrosine kinase. Oncogene 1993, 8, 909-918.
34. Rosnet O, Stephenson D, Matteti MG, Marchetto, S, Shibuya M, Chapman V, Birnbaum D. Close physical linkage of the FLT1 and FLT3 genes on chromosome 13 in man and chromosome 5 in mouse. Oncogene 1993, 8, 173-179.
35. Rosnet O, Schiff C, Pebusque M-J, Marchetto S, Tonnelle C, Toiron Y, Birg F, Birnbaum D. Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993, 82, 1110-1119.
36. Birg F, Rosnet O, Carbuccia N, Birnbaum D. The expression of FMS, KIT and FLT3 in hematopoietic malignancies. Leukemia Lymphoma 1994, 13, 223-227.
37. Dosil M, Wang S, Lemischka I. Mitogenic signaling and substrate specificity of the flk2/flt3 receptor tyrosine kinase in fibroblast and interleukin-3-dependent hematopoietic cells. Mol Cell Biol 1993, 13, 6572-6585.
38. Lyman SD, James L, Escobar S, Downey H, de Vries K, Brasel K, Stocking K, Beckmann MP, Copeland NG, Cleveland LS, Jenkins NA, Belmont JW, Davison B. Identification of soluble and membrane-bound isoforms of the murine flt3 ligand generated by alternative splicing of mRNAs. Oncogene 1995, 10, 149-157.
39. Lyman SD, Stocking K, Davison B, fletcher F, Johnson L, Escobar S. Structural analysis of human and murine flt3 ligand genomic loci. Oncogene 1995, 11, 1165-1172.
40. Anderson DM, Lyman SD, Baird A, Wignall JM, Eisenman J, Rauch C, March CJ, Boswell HS, Gimpel SD, Cosman D, Williams DE. Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms. Cell 1990, 63, 235-243.
41. Huang EJ, Nocka KH, Buck J, Besmer P. Differential expression and processing of two cell associated forms of the kit-ligand: KL-1 and KL-2. Mol Biol Cell 1992, 3, 346-362.
42. Martin FH, Suggs SV, Langley KE, Lu HS, Ting J, Okino KH, Morris CF, McNiece IK, Jacobsen FW, Mendiaz EA, Birkett NC, Smith KA, Johnson MJ, Parker VP, Flores JC, Patel AC, Fisher EF, Erjavec HO, Herrera CJ, Wypych J, Sachdev RK, Pope JA, Leslie I, Wen D, Lin C-H, Cupples RL, Zsebo KM. Primary structure and functional expression of rat and human stem cell factor DNAs. Cell 1990, 63, 203-211.
43. Brannan CI, Bedell MA, Resnick JL, Eppig JJ, Handel MA, Williams DE, Lyman SD, Donovan PJ, Jenkins NA, Copeland NG. Developmental abnormalities in Steel 7H mice result from a splicing defect in the steel factor cytoplasmic tail. Genes and Development 1992, 6, 1832-1842.
44. Ladner MB, Martin GA, Noble JA, Nikoloff DM, Tal R, Kawasaki ES, White TJ. Human CSF-1: gene structure and alternative splicing of mRNA precursor. EMBO J 1987, 6, 2693-2698.
45. Stein J, Borzillo GV, Rettenmier CW. Direct stimulation of cell expressing receptors for macrophage colony-stimulating factor (CSF-1) by a plasma membrane-bound precursor of human CSF-1. Blood 1990, 76, 1308-1314.
46. McKenna HJ, de Vries P, Brasel K, Lyman SD, Williams DE. Effect of flt3 ligand on the ex vivo expansion of human CD34+ hematopoietic progenitor cells. Blood 1995, 86, 3413-3420.
47. Zeigler FC, Bennett BD, Jordan CT, Spencer SD, Baumhueter S, Carroll KJ, Hooley J, Bauer K, Matthews W. Cellular and molecular characterization of the role of the flk-2/flt-3 receptor tyrosine kinase in hematopoietic stem cells. Blood 1994, 84, 2422-2430.
48. DaSilva N, Hu Z, Ma W, Rosnet O, Birnbaum D, Drexler H. Expression of the FLT3 gene in human leukemia-lymphoma cell lines. Leukemia 1994, 8, 885-888.
49. Rambaldi A, Wakamiya N, Vellenga E, Horiguchi J, Warren MK, Kufe D, Griffin JD. Expression of the macrophage colony-stimulating factor and c-fms genes in human acute myeloblastic leukemia cells. J Clin Invest 1989, 81, 1030-1035.
50. Wang C, Curtis J, Geissler E, McCulloch E, Linden M. The expression of the proto-oncogene c-kit in the blast cells of acute myeloblastic leukemia. Leukemia 1989, 3, 699-701.
51. Ikeda H, Kanakura Y, Tamaki T, Kuriu A, Kitayama H, Ishikawa J, Kanayama Y, Yonezawa T, Taruim S, Griffin JD. Expression and functional role of the proto-oncogene c-kit in acute myeloblastic leukemia cells. Blood 1991, 78, 2962-2968.
52. Birg F, Courcoul M, Rosnet O, Bardin F, Pebusque MJ, Marchetto S, Tabilio A, Mannoni P, Birnbaum D. Expression of the FMS/KIT-like gene FLT3 in human acute leukemias of the myeloid and lymphoid lineages. Blood 1992, 80, 2584-2593.
53. Turner AM, Lin NL, Issarachai S, Lyman SD, Broudy VC. FLT3 receptor expression on the surface of normal and malignant human hematopoietic cells. Blood 1996, 88, 3383-3390.
54. Meierhoff G, Dehmel U, Gruss H-J, Rosnet O, Birnbaum D, Quentmeier H, Dirks W, Drexler HG. Expression of FLT3 receptor and FLT3-ligand in human leukemia-lymphoma cell lines. Leukemia 1995, 9, 1368-1372.
55. Kuczynski WI, Ratajczak MZ. STK-1 receptor gene is expressed in various human non-hematopoietic tumor cell lines: RT-PCR directed analysis of STK-1 mRNA expression. Preliminary report. Acta Haematologica Polonica 1994, 25, 43-46.
56. Brasel K, Escobar S, Anderberg R, de Vries P, Gruss H-J, Lyman SD. Expression of the flt3 receptor and its ligand on hematopoietic cells. Leukemia 1995, 9, 1212-1218.
57. Rogers SY, Bradbury D, Kozlowski R, Russel NH. Evidence for internal autocrine regulation of growth in acute myeloblastic leukemic cells. Exp Hematol 1994, 22, 593-598.
58. Shah A, Smogorzewska E, Hannum C, Crooks G. Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38- cells and maintains progenitor cells in vitro. Blood 1996, 9, 3563-3570.
59. Jacobsen SEW, Okkenhaug C, Myklebust J, Veiby OP, Lyman SD. The FLT3 ligand potently and directly stimulates the growth and expansion of primitive murine bone marrow progenitor cells in vitro: Synergistic interactions with interleukin (IL) 11, IL-12, and other hematopoietic growth factors. J Exp Med 1995, 181, 1357-1363.
60. Gabbianelli M, Pelosi E, Montesoro E, Valtieri M, Luchetti L, Samoggia P, Vitelli L, Barberi T, Testa U, Lyman S, Peschle C. Multi-level effects of flt3 ligand on human hematopoiesis: Expansion of putative stem cells and proliferation of granulomonocytic progenitors/monocytic precursors. Blood 1995, 86, 1661-1670.
61. Brasel K, McKenna HJ, Morrissey PJ, Charrier K, Morris AE, Lee CC, Williams DE, Lyman SD. Hematologic effects of flt3 ligand in vivo in mice. Blood 1996, 88, 2004-2012.
62. Lyman SD, Maraskovsky E, McKenna HJ. Flt3 Ligand. In the Cytokine Handbook, Thomson AW (Ed). Academic Press, San Diego (in press), 1998.
63. Veiby OP, Jacobsen FW, Cui L, Lyman SD, Jacobsen SE. The flt3 ligand promotes the survival of primitive hemopoietic progenitor cells with myeloid as well as B lymphoid potential. Suppression of apoptosis and counteraction by TNF-alpha and TGF-beta. J Immunol 1997, 157, 2953-2960.
64. Muench MO, Roncarolo MG, Menon S, Xu Y, Kastelein R, Zurawski S, Hannum CH, Culpepper J, Lee F, Namikawa R. FLK-2/FLT-3 ligand regulates the growth of early myeloid progenitors isolated from human fetal liver. Blood 1995, 85, 963-972.
65. Muench MO, Schneider JG, Moore MAS. Interactions among colony-stimulating factors, IL-1β, IL-6 and kit-ligand in the regulation of primitive murine hematopoietic cells. Exp Hematol 1992, 20, 339-349.
66. McNiece IK, Langley KE, Zsebo KM. Recombinant human stem cell factor synergises with GM-CSF, G-CSF, IL-3 and Epo to stimulate the human progenitor cells of the myeloid and erythroid lineages. Exp Hematol 1991, 19, 226-231.
67. Brandt J, Briddell RA, Srour EF, Leemhuis TB, Hoffman R. Role of c-kit ligand in the expansion of human hematopoietic progenitor cells. Blood 1992, 769, 634-641.
68. Koller MR, Oxender M, Brott DA, Palsson BO. Flt-3 ligand is more potent than c-kit ligand for the synergistic stimulation of ex vivo hematopoietic cell expansion. J Hematother 1996, 5, 449-459.
69. Petzer AL, Zandstra PW, Piret JM, Eaves CJ. Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin. J Exp Med 1996, 183, 2551-2558.
70. Ohmizono Y, Sakabe H, Kimura T, Tanimukai S, Matsumura T, Miyazaki H, Lyman SD, Sonoda Y. Thrombopoietin augments ex vivo expansion of human cord blood-derived hematopoietic progenitors in combination with stem cell factor and flt3 ligand. Leukemia 1997, 11, 524-530.
71. Jacobsen SE, Veiby OP, Myklebust J, Okkenhaug C, Lyman SD. Ability of flt3 ligand to stimulate the in vitro growth of primitive murine hematopoietic progenitors is potently and directly inhibited by transforming growth factor-beta and tumor necrosis factor-alpha. Blood 1996, 87, 5016-5026.
72. Broxmeyer H, Lu L, Cooper S, Ruggieri L, Li Z, Lyman S. Flt3 ligand stimulates/costimulates the growth of myeloid stem/progenitor cells. Exp Hematol 1995, 23, 1121-1129.
73. Hirayama F, Lyman SD, Clark SC, Ogawa M. The flt3 ligand supports proliferation of lymphohematopoietic progenitors and early B-lymphoid progenitors. Blood 1995, 85, 1762-1768.
74. Hudak S, Hunte B, Culpepper J, Menon S, Hannum C, Trompson-Snipes L, Rennick D. FLT3/FFLK2 ligand promotes the growth of murine stem cells and the expansion of colony-forming cells and spleen colony-forming units. Blood 1995, 85, 2747-2755.
75. Hirayama F, Clark SC, Ogawa M. Negative regulation of early B-lymphopoiesis by interleukin-3 and interleukin-1α. Proc Natl Acad Sci USA 1994, 91, 469-473.
76. Hunte BE, Hudak S, Campbell D, Xu Y, Rennick D. Flk2/flt3 ligand is a potent cofactor for the growth of primitive B cell progenitors. J Immunol 1996, 156, 489- 496.
77. Freedman AR, Zhu H, Levine JD, Kalams S, Scadden DT. Generation of human T lymphocytes from bone marrow CD34+ cells in vitro. Nature Medicine 1996, 2, 46- 51.
78. Brugger W, Mocklin W, Heimfeld S, Berenson RJ, Mertelsmann R, Kanz L. Ex vivo expansion of enriched periferal blood CD34- progenitor cells by stem cell factor, interleukin 1β (IL-1β), IL-6, IL-3, interferon-γ, and erytropoietin. Blood 1993, 81, 2579-2584.
79. Caramatti C, Carlo-Stella C, Mangoni L, Garau D, Regazzi E, Almici C, Rizzoli V. Role of FLT3 ligand in the ex vivo amplification of G-CSF-mobilized CD34+CD45RA- hematopoietic progenitors. Proc ASCO 1996, 15, 277.
80. Shurin MR, Pandharipande PP, Zorina TD, Haluszczak C, Subbotin VM, Hunter O, Brumfield A, Storkus WJ, Maraskovsky E, Lotze MT. FLT3 Ligand induces the generation of functionally active dendritic cells in mice. Cell Immunol 1997, 179, 174-184.
81. Maraskovsky E, Brasel K, Teepe M, Roux E, Shortman K, Lyman SD, McKenna HJ. In vivo administration of flt3 ligand results in the generation of large numbers of dendritic cells in the lymphoid tissue of mice. J Exp Med 1996, 184, 1953-1962.
82. Steinman RM. The dendritic cell system and its role in immunogenicity. Ann Rev Immunol 1991, 9, 271-296.
83. Matzinger P, Guerder S. Does T cell tolerance require a dedicated antigen-presenting cell? Nature 1989, 338, 74-76.
84. Shortman K, Wu L, Ardavin C, Vremec D, Sotzik F, Winker K, Suss G. Thymic dendritic cells: surface phenotype, developmental origin and function. In Banchereau J and Schmitt D. (eds),Dendritic Cells in Fundamental and Clinical Immunology, Vol. 2, Plenum Press, New York, 1995, pp. 21-29.
85. Agger R, Crowley MT, Witmer-Pack MD. The surface of dendritic cells in the mouse as studied with monoclonal antibodies Int Rev Immunol 1990, 6, 89-101.
86. Szabolcs P, Moore MAS, Young JW. Expansion of immunostimulatory dendritic cells among the myeloid progeny of human CD34+ bone marrow precursors cultured with c-kit ligand, GM-CSF, and TNF-α. J Immuno 1995, 154, 5851-5861.
87. Crowley M, Inaba K, Steinman RM. Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins. J Exp Med 1990, 172, 383-386.
88. Grabbe S, Bruvers S, Gallo RL, Knisely TL, Nazareno R, Granstein RD. Tumor antigen presentation by murine epidermal cells. J Immunol 1991, 146, 3656-3661.
89. Grabbe S, Beissert S, Schwarz T, Granstein RD. Dendritic cells as initiators of tumor immune responses: a possible strategy for tumor immunotherapy. Immun Today 1995, 16, 117-121.
90. Mayordomo JI, Zorina T, Storkus WJ, Zitvogel L, Celluzzi C, Falo LD, Melief CJ, Ilstad ST, Martin KW, DeLeo AB, Lotze MT. Bone marrow-derived dendritic cells pulsed with synthetic tumour peptides elicit protective and therapeutic antitumor immunity. Nature Medicine 1995, 1, 1297-1302.
91. Zitvogel L, Mayordomo J, Tjandrawan T, DeLeo A, Clarke M, Lotze M, Storkus W. Therapy of murine tumors with tumor peptide-pulsed dendritic cells: Dependence on T cells, B7 costimulation, and T helper cell 1-associated cytokines. J Exp Med 1996, 183, 87-97.
92. Inaba K, Steinmann RM, Pack MW, Aya H, Inaba M, Sudo T, Wolpe S, Schuler G. Identification of proliferating dendritic cell precursor in mouse blood J Exp Med 1992, 175, 1157-1167.
93. Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch PO, Steinman RM, Schuler G. Proliferating dendritic cell progenitors in human blood. J Exp Med 1994, 180, 83-93.
94. Sallusto F, Lanzavecchia A. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 downregulated by tumor necrosis factor alpha. J Exp Med 1994, 179, 1109-1118.
95. Siena S, DiNicola M, Mortarini R, Anichini A, Bregni M, Parmiani G, Gianni AM. Efficient ex vivo generation of functional dendritic cells (DCs) utilizable for tumor vaccination from blood cell transplants (BCT) in cancer patients. Proc ASCO 1996, 15, 554.
96. Shurin MR, Pandharipande PP, Sikora SS, Haluszczak K, Maraskovsky E, Storkus W, Lotze MT. Characterization of dendritic cells obtained from mice treated with FLT3-ligand and IL-12. Abs 4th Intl Symposium on Dendritic Cells Venice, Italy, Oct. 1996.
97. Shurin MR. Dendritic cells presenting tumor antigen. Cancer Immunol Immunother 1996, 43, 158-164.
98. Lynch DH, Andreasen A, Maraskovsky E, Whitmore J, Miller RE, Schuh JCL. Flt3 ligand induces tumor regression and antitumor immune responses in vivo. Nature Med 1997; 3, 625-631.
99. Shurin MR, Peron JM, Esche C, Haluszczak C, Lotze MT. Induction of dendritic cell generation in vivo by FLT3-Ligand: a novel immunotherapeutic tool for cancer treatment. Proceedings of Euro cancer 97, 1997, 10, 299a.
100. Peron JM, Shurin MR, Lotze MT. Treatment of murine subcutaneous and metastatic tumors with FLT3-Ligand: a new approach in dendritic cell based immunotherapies. Proceedings of Euro cancer 97, 1997, 10, 300a.
101. Esche C, Shurin MR, Peron JM, Lotze MT. FLT3 Ligand - a new cytokine for immunotherapy of melanoma. Pig Cell Res (in press), 1997 (abstr).
102. Chen K, Braun SE, Wiebke E, Lyman SD, Chiang Y, Broxmeyer HE, Cornetta K. Retroviral-mediated gene transfer of the flt3 ligand into murine breast cancer cells prevents tumor growth in vivo. Blood 1995, 86 (Suppl 1), 244a.
103. Averbook B, Maliszewski C, Schuh J, Papay R. Flt3 Ligand antitumor activity to a murine adenocarcinoma improves cellular adoptive immunotherapy. Proceedings of the AACR 1997, 38, 402a.