Different efficacy of specific agonists at 5-HT3 receptor splice variants: The role of the extra six amino acid segment

British Journal of Pharmacology

Volumn 123, Issue 4, 1998, Pages 661-666

  Niemeyer, M I ^a   Lummis, S C R ^b,c  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

5 HT3 receptor; Efficacy; Ligand gated ion channel; Patch clamp; Radioligand binding; Splice variants; Stable transfection

Indexed keywords

1 (3 CHLOROPHENYL)BIGUANIDE; 1-(3-CHLOROPHENYL)BIGUANIDE; 2 METHYL 5 HT; 2-METHYL-5-HT; BIGUANIDE DERIVATIVE; DRUG DERIVATIVE; SEROTONIN; SEROTONIN 3 RECEPTOR; SEROTONIN AGONIST; SEROTONIN RECEPTOR;

ARTICLE; CELL LINE; CHEMISTRY; GENETICS; HUMAN; METABOLISM; RADIOASSAY; RNA SPLICING; SITE DIRECTED MUTAGENESIS;

BIGUANIDES; CELL LINE; HUMANS; MUTAGENESIS, SITE-DIRECTED; RADIOLIGAND ASSAY; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT3; RNA SPLICING; SEROTONIN; SEROTONIN AGONISTS;

EID: 0031989118     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0701657     Document Type: Article

References (42)

1. 3H]granisetron, Biochem. Pharmacol., 45, 2155 - 2158.
2. 3 receptor. J. Neurochem., 64, 1401 - 1405.
3. BOESS, F.G. & MARTIN, I.L. (1994). Molecular biology of 5-HT receptors. Neuropharmacology, 33, 275 - 317.
4. 3 receptors in NG108-15 neuroblastoma x glioma cells: effect of the novel agonist 1-(m-chlorophenyl)-biguanide. Neuropharmacology, 31, 561 - 567.
5. BRADFORD, M.M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248 - 254.
6. A receptor and compromises the modulatory interactions. Eur. J. Pharmacol., 73, 65 - 73.
7. CHANGEUX, J.-P., GIRAUDAT, J. & DENNIS, M. (1987). The nicotinic acetylcholine receptors, molecular architecture of a ligand-regulated ion channel. Trends Pharmacol. Sci., 8, 459 - 465.
8. CHEN, D. & OKAYAMA, H. (1987). Calcium phosphate mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA. Biotechniques, 6,632 - 638.
9. COOPER, S.T. & MILLAR, N.S. (1997). Host cell-specific folding and assembly of the neuronal nicotinic acetylcholine receptor α7 subunit. J. Neurochem., 68, 2140 - 2151.
10. 3 receptors and membrane ion channels. Nature. 339, 706 - 709.
11. 3 R-A subunit expressed in Xenopus laevis oocytes. Neuropharmacology, 33, 473 - 482.
12. 3 receptor-A mRNA in NG108-15 cells.
13. 3 receptor stably expressed in HEK293 cells. Br. J. Pharmacol., 114, 1211 - 1221.
14. 3 receptor splice variants isolated from a mouse hippocampal cell line. Pflugers Arch. Eur. J. Physiol., 432, 134 - 143.
15. 3 receptors provides new insights into their maturation and structure. J. Biol. Chem., 270, 6056 - 6061.
16. 2+ channels. Mol. Pharmacol., 50, 1284 - 1294.
17. 2+ permeability of cloned and native 5-hydroxytryptamine type 3 receptors. Mol. Pharmacol., 46, 1120 - 1128.
18. 3 receptor A subunit. Eur. J. Pharmacol., 245, 187 - 192.
19. HUGANIR, R.L. & GREENGARD, P. (1990). Regulation of neurotransmitter receptor desensitization by protein phosphorylation. Neuron, 5, 555 - 567.
20. JONES, M.V. & WESTBROOK, G.L. (1996). The impact of receptor desensitization on fast synaptic transmission. Trends Neurosci., 19,96 - 101.
21. A receptor function by protein kinase C phosphorylation. Neuron, 12, 1081 - 1095.
22. KUNKEL, T.A. (1985). Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. U.S.A., 82, 488 - 492.
23. 3 receptors in clonal cell lines studied by patch clamp techniques. Br. J. Pharmacol., 97, 27 - 40.
24. 3 receptors in intact N1E-115 neuroblastoma cells. Eur. J. Pharmacol., 189, 223 - 227.
25. 3 receptors in N1E-115 neuroblastoma cells. Eur. J. Pharmacol., 243, 7 - 11.
26. 3 receptor, a serotonin-gated ion channel. Science, 254, 432 - 435.
27. MCPHERSON, G.A. (1985). Analysis of radioligand binding experiments: A collection of computer programes for the IBM PC. J. Pharmacol. Methods, 14, 213 - 227.
28. 3 receptor splice variants in the rat. J. Neurochem., 65, 475 - 483.
29. 3 receptor: heterogeneity in distribution and function among species. Mol. Pharmacol., 48, 407 - 416.
30. 3 receptors: focus on alcohols and anaesthetic agents. Trends Pharmacol. Sci., 17, 95 - 99.
31. 3 receptors-induced currents in N1E-155 neuroblastoma cells. Eur. J. Pharmacol., 151, 491 - 495.
32. PINNA, L.A. (1990). Casein kinase 2: an 'eminence grise' in cellular regulation? Biochim. Biophys. Acta. 1054, 267 - 284.
33. 3 receptors in N1E-115 neuroblastoma cells. Br. J. Pharmacol., 104, 536 - 540.
34. 3 receptor. J. Neurophysiol., 65, 630 - 638.
35. SUZUKI, S., TACHIBANA, M. & KANEKO, A. (1990). Effects of glycine and GABA on isolated bipolar cells of the mouse retina. J. Physiol., 421, 645 - 651.
36. 3 receptor-gated ion channel events resolved in N1E-115 mouse neuroblastoma cells. Biochem. Biophys. Res. Commun., 199, 227 - 233.
37. 3 receptor ligand-gated ion channels. Receptors Channels, 3, 7 - 12.
38. 3 receptor by full and partial agonists. Br. J. Pharmacol., 117, 839 - 846.
39. A receptor expressed in Xenopus oocytes requires 8 amino acids contained in the γ2L subunit. Neuron, 7, 27 - 33.
40. A receptors containing the α4 subunit. Mol. Pharmacol., 50, 670 - 678.
41. 3 receptor gene and functional expression of two splice variants. Mol. Brain Res., 26, 233 - 241.
42. 3 receptor-mediated responses by protein kinase C activation. NeuroReport, 6, 1464 - 1468.