Functional characterization of two 5-HT3 receptor splice variants isolated from a mouse hippocampal cell line

Pflugers Archiv European Journal of Physiology

Volumn 432, Issue 1, 1996, Pages 134-143

  Glitsch, Maike ^a   Wischmeyer, Erhard ^a   Karschin, Andreas ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

HN9.10; Ligand gated channel; Neuroblastoma cell; Polymerase chain reaction; Recombinant receptor; Serotonin receptor; Xenopus oocytes

Indexed keywords

1 METHYL 3 INDAZOLECARBOXYLIC ACID N TROPANYLAMIDE; LIGAND; PHENYLBIGUANIDE; PROTEIN KINASE C; SEROTONIN; SEROTONIN 3 RECEPTOR;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; GENE EXPRESSION; HIPPOCAMPUS; ION PERMEABILITY; MOUSE; NEUROBLASTOMA CELL; NONHUMAN; OOCYTE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; XENOPUS LAEVIS;

ANIMALS; BASE SEQUENCE; CALCIUM; CLONING, MOLECULAR; DNA, RECOMBINANT; ENZYME ACTIVATION; HIPPOCAMPUS; INJECTIONS; ION CHANNEL GATING; ION CHANNELS; MICE; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; OOCYTES; PROTEIN KINASE INHIBITORS; PROTEIN KINASES; RECEPTORS, SEROTONIN; RECOMBINANT PROTEINS; RNA, COMPLEMENTARY; SEROTONIN; TUMOR CELLS, CULTURED; VARIATION (GENETICS);

EID: 0029902712     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050115     Document Type: Article

References (34)

1. 3 receptors in NG108-15 neuroblastoma x glioma hybrid cells. J Physiol (Lond) 473:267P
2. 3 receptors are membrane ion channels. Nature 339:706-709
3. 3R-A subunit expressed in Xenopus laevis oocytes. Neuropharmacology 33:473-482
4. Eckstein F (1985) Nucleoside phosphorothioates. Annu Rev Biochem 54:367-402
5. Eiselé JL, Bertrand S, Galzi JL, Devillers-Thiéry A, Changeux JP, Bertrand B (1993) Chimaeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities. Nature 366:479-483
6. 3R-A) expressed in HEK 293 cells. Br J Pharmacol 109:98P
7. 2+ levels. Receptors and Channels 3:83-88
8. Goldin AL (1992) Maintenance of Xenopus laevis and oocyte injection. In: Rudy B, Iverson LE (eds) Methods in enzymology (207). Academic, San Diego, pp 266-279
9. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85-100
10. Hille B (1992) Ionic channels of excitable membranes. Sinauer Associates, Sunderland, Mass., pp 337-361
11. 3 receptor A subunit. Eur J Pharmacol 245:187-192
12. Hoyer D, Clarke DE, Fozard JR, Hartig PR, Martin GR, Mylecharane EJ, Saxena PR, Humphrey PPA (1994) International union of pharmacology classification of receptors for 5-hydrxytryptamine (serotonin). Pharmacol Rev 46: 157-203
13. Hussy N, Lukas W, Jones KA (1994) Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors. J Physiol (Lond) 481: 311-323
14. Imoto K, Busch C, Sakmann B, Mishina M, Konno T, Nakai J, Bujo H, Mori Y, Fukuda K, Numa S (1988) Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance. Nature 335:645-648
15. Fee HJ, Hammond DN, Large TH, Roback JD, Sim JA, Brown DA, Otten UH Wainer BH (1990) Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice. J Neurosci 10: 1779-1787
16. Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Habor Laboratory, Cold Spring Harbor, N.Y.
17. 3receptor, a serotonin-gated ion channel. Science 254:432-437
18. 3 receptors in NG108-15 clonal cells. Biochem Pharmacol 42:1453-1461
19. 3 receptor splice variants in the rat. J Neurochem 65:475-483
20. Pearson RB, Kemp BE (1991) Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations. In: Hunter T, Sefton BM (eds) Methods in enzymology (200). Academic, San Diego, pp 62-81
21. 3 receptor-induced currents in NIE-115 neuroblastoma cells. Eur J Pharmacol 151:491-495
22. Robertson B, Bevan S (1991) Properties of 5-hydroxytryptamine 3 receptor-gated currents in adult rat dorsal root ganglion neurons. Br J Pharmacol 102:272-276
23. Swope SL, Moss SJ, Blackstone CD, Huganir RL (1992) Phosphorylation of ligand-gated ion channels: a possible mode of synaptic plasticity. FASEB J 6:2514-2523
24. Toullec D, Pianetti P, Coste H, Bellevergue P, Grand-Perret T, Ajakane M, Baudet V, Boissin P, Boursier E, Loriolle F, Duhamel L, Charon D, Kirilovsky J (1991) The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem 24:15771-15781
25. 3 receptor gene and assignment to human chromosome II. FEBS Lett 339: 302-306
26. 3 receptor ligand-gated ion channels. Receptors Channels 3:7-12
27. 3 receptor gene and functional expression of two splice variants. Mol Brain Res 26:233-241
28. 3 receptors. Academic, New York
29. 3 receptors mediate rapid responses in cultured hippocampus and a clonal cell line. Neuron 1:615-621
30. 3 receptor. Brain Res 533:46-52
31. 3receptor in a rat glioma x mouse neuroblastoma hybrid cell. J Physiol (Lond) 436:293-308
32. Yang J (1990) Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells. J Gen Physiol 96:1177-1198
33. 3 receptor channels in dissociated rat superior cervical ganglion neurons. J Physiol (Lond) 448:237-256
34. 3 receptor-mediated responses by protein kinase C activation. Neuroreport 6:1336-1340