Optimized BlaM-transposon shuttle mutagenesis of Helicobacter pylori allows the identification of novel genetic loci involved in bacterial virulence

Molecular Microbiology

Volumn 20, Issue 2, 1996, Pages 361-373

  Odenbreit, Stefan ^a   Till, Markus ^a   Haas, Rainer ^a  

^a MAX PLANCK INST FÜR BIOLOGIE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL VIRULENCE; CONTROLLED STUDY; GENE LIBRARY; GENE LOCUS; HELICOBACTER PYLORI; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; SHUTTLE VECTOR; TRANSPOSON;

EID: 0029881277     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02623.x     Document Type: Article

References (45)

1. Ascencio, F., Fransson, L.A., and Wadström, T. (1993) Affinity of the gastric pathogen Helicobacter pylori for the N-sulphated glycosaminoglycan heparin sulphate. J Med Microbiol 38: 240-244.
2. Borén, T., Falk, P., Roth, K.A., Larson, G., and Normark, S. (1993) Attachment of Helicobacter pylori to gastric epithelium mediated by blood group antigens. Science 262: 1892-1895.
3. Boyer, H.W., and Roulland-Dussoix, D. (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41: 459-472.
4. Bukanov, N.O., and Berg, D.E. (1994) Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC11638. Mol Microbiol 11: 509-523.
5. Clyne, M., and Drumm, B. (1993) Adherence of Helicobacter pylori to primary human gastrointestinal cells. Infect Immun 61: 4051-4057.
6. Covacci, A., et al (1993) Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA 90: 5791-5795.
7. Cover, T.L., Tummuru, M.K.R., Cao, P., Thompson, S., and Blaser, M.J. (1994) Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J Biol Chem 269: 10566-10573.
8. Devereux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the Vax. Nucl Acids Res 12: 387-395.
9. Doig, P., Austin, J.W., and Trust, T.J. (1993) The Helicobacter pylori 19.6-kilodalton protein is an iron-containing protein resembling ferritin. J Bacteriol 175: 557-560.
10. Donnenberg, M.S., Calderwood, S.B., Donohue-Rolfe, A., Keusch, G.T., and Kaper, J.B. (1990) Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade Hep-2 cells. Infect Immun 58: 1565-1571.
11. Dubnau, D. (1991) Genetic competence in Bacillus subtilis. Microbiol Rev 55: 395-424.
12. Eaton, K.A., and Krakowa, S. (1994) Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect Immun 62: 3604-3607.
13. Eaton, K.A., Morgan, D.R., and Krakowka, S. (1992) Motility as a factor in the colonisation of gnotobiotic piglets by Helicobacter pylori. J Med Microbiol 37: 123-127.
14. Elkins, C., Thomas, C.E., Seifert, H.S., and Sparling, P.F. (1991) Species-specific uptake of DNA by gonococci is mediated by a 10-base-pair sequence. J Bacteriol 173: 3911-3913.
15. Elomar, E., Penman, I., Dorrian, C.A., Ardill, J.E.S., and McColl, K.E.L. (1993) Eradicating Helicobacter pylori infection lowers gastrin mediated acid secretion by two thirds in patients with duodenal ulcer. Gut 34: 1060-1065.
16. Falk, P., Roth, K.A., Borén, T., Westblom, T.U., Gordon, J.I., and Normark, S. (1993) An in vitro adherence assay reveals that Helicobacter pylori exhibits cell lineage-specific tropism in the human gastric epithelium. Proc Natl Acad Sci USA 90: 2035-2039.
17. Finlay, B.B., Starnbach, M.N., Francis, C.L., Stocker, B.A.D., Chatfield, S., Dougan, G., and Falkow, S. (1988) Identification and characterization of TnphoA mutants of Salmonella that are unable to pass through a polarized MDCK epithelial cell monolayer. Mol Microbiol 2: 757-766.
18. Geider, K., Hohmeyer, C., Haas, R., and Meyer, T.F. (1985) A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd. Gene 33: 341-349.
19. Haas, R., Kahrs, A.F., Facius, D., Allmeier, H., Schmitt, R., and Meyer, T.F. (1993a) TnMax - a versatile mini-transposon for the analysis of cloned genes and shuttle mutagenesis. Gene 130: 23-31.
20. Haas, R., Meyer, T.F., and van Putten, J.P.M. (1993b) Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis. Mol Microbiol 8: 753-760.
21. Josenhans, C., Labigne, A., and Suerbaum, S. (1995) Comparative ultrastructural and functional studies of Helicobacter pylori and Helicobacter mustelae flagellin mutants: both flagellin subunits, FlaA and FlaB, are necessary for full motility in Helicobacter species. J Bacteriol 177: 3010-3020.
22. Kahrs, A.F., Bihlmaier, A., Facius, D., and Meyer, T.F. (1994) Generalized transposon shuttle mutagenesis in Neisseria gonorrhoeae: a method for isolating epithelial cell invasion-defective mutants. Mol Microbiol 12: 819-831.
23. Kahrs, A.F., Odenbreit, S., Schmitt, W., Heuermann, D., Meyer, T.F., and Haas, R. (1995) An improved TnMax mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions. Gene 167: 53-57.
24. Labigne, A., Cussac, V., and Courcoux, P. (1991) Shuttle cloning of genes responsible for urease activity from Helicobacter pylori and nucleotide sequence determination. J Bacteriol 173: 1920-1931.
25. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680-685.
26. Langton, S.R. (1992) Helicobacter pylori associated phospholipase-A2 activity: a factor in peptic ulcer production? J Clin Pathol 45: 221-224.
27. Leying, H., Suerbaum, S., Geis, G., and Haas, R. (1992) Characterisation of flaA, a Helicobacter pylori flagellin gene. Mol Microbiol 6: 2863-2874.
28. Lingwood, C.A., Huesca, M., and Kuksis, A. (1992) The glycerolipid receptor for Helicobacter pylori (and exoenzyme-S) is phosphatidylethanolamine. Infect Immun 60: 2470-2474.
29. Manoil, C., and Beckwith, J. (1985) TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci USA 82: 8129-8133.
30. Nomura, A., Stemmermann, G.N., Chyou, P.H., Kato, I., Perez-Perez, G.I., and Blaser, M.J. (1991) Helicobacter pylori infection and gastric carcinoma among Japanese americans in Hawaii. N Engl J Med 325: 1132-1136.
31. O'Toole, P.W., Kostrzynska, M., and Trust, T.J. (1994) Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production. Mol Microbiol 14: 691-703.
32. Phadnis, S.H., Ilver, D., Janzon, L., Normark, S., and Westblom, T.U. (1994) Pathological significance and molecular characterization of the vacuolating cytotoxin gene of Helicobacter pylori. Infect Immun 62: 1557-1565.
33. Pifer, M.L., and Smith, H.O. (1985) Processing of donor DNA during Haemophilus influenzae transformation: analysis using a model plasmid system. Proc Natl Acad Sci USA 82: 3731-3735.
34. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
35. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
36. Schmitt, W., and Haas, R. (1994) Genetic analysis of the Helicobacter pylori vacuolating cytotoxin: structural similarities with the IgA protease type of exported protein. Mol Microbiol 12: 307-319.
37. Slomiany, B.L., Murty, V.L.N., Piotrowski, J., Liau, Y.H., Sundaram, P., and Slomiany, A. (1992) Glycosulfatase activity of Helicobacter pylori toward gastric mucin. Biochem Biophys Res Commun 183: 506-513.
38. Suerbaum, S. (1995) The complex flagella of gastric Helicobacter species. Trends Microbiol 3: 168-170.
39. Suerbaum, S., Josenhans, C., and Labigne, A. (1993) Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA and flaB-negative mutants by electroporation-mediated allelic exchange. J Bacteriol 175: 3278-3288.
40. Tadayyon, M., and Broome-Smith, J.K. (1992) TnblaM - a transposon for directly tagging bacterial genes encoding cell envelope and secreted proteins. Gene 111: 21-26.
41. Taylor, D.N., and Blaser, M.J. (1991) The epidemiology of Helicobacter pylori infection. Epidemiol Rev 13: 42-59.
42. Taylor, R.K., Miller, V.L., Furlong, D.B., and Mekalanos, J.J. (1987) Use of pnoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci USA 84: 2833-2837.
43. Telford, J.L., Covacci, A., Ghiara, P., Montecucco, C., and Rappuoli, R. (1994) Unraveling the pathogenic role of Helicobacter pylori in peptic ulcer: potential new therapies and vaccines. Trends Biotechnol 12: 420-426.
44. Trust, T.J., Doig, P., Emody, L., Kienle, Z., Wadström, T., and O'Toole, P. (1991) High-affinity binding of the basement membrane proteins collagen type-IV and laminin to the gastric pathogen Helicobacter pylori. Infect Immun 59: 4398-4404.
45. Wotherspoon, A.C., Doglioni, C., Diss, T.C., Pan, L., Moschini, A., De-Boni, M., and Isaacson, P.G. (1993) Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet 342: 575-577.