Analysis of the Legionella pneumophila fliI gene: Intracellular growth of a defined mutant defective for flagellum biosynthesis

Infection and Immunity

Volumn 65, Issue 6, 1997, Pages 2497-2501

  Merriam, Jennifer J ^a   Mathur, Ruchi ^a   Maxfield Boumil, Rebecca ^a   Isberg, Ralph R ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GROWTH; BACTERIAL MUTATION; BACTERIAL VIRULENCE; FLAGELLUM; GENE SEQUENCE; LEGIONELLA PNEUMOPHILA; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE;

EID: 0030994460     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.6.2497-2501.1997     Document Type: Article

References (46)

1. Barbaree, J. M., B. S. Fields, J. C. Feeley, G. W. Gorman, and W. T. Martin. 1986. Isolation of protozoa from water associated with a legionellosis out- break and demonstration of intracellular multiplication of Legionella pneumophila. Appl. Environ. Microbiol. 51: 422-424.
2. Berger, K. H., and R. R. Isberg. 1993. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. Mol. Microbiol. 7:7-19.
3. Berger, K. H., J. J. Merriam, and R. R. Isberg. 1994. Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene. Mol. Microbiol. 14: 809-822.
4. Blander, S. J., R. F. Breiman, and M. A. Horwitz. 1989. A live avirulent mutant Legionella pneumophila vaccine induces protective immunity against lethal aerosol challenge. J. Clin. Invest. 83: 810-815.
5. Bolivar, F. 1978. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4: 121-136.
6. Brand, B. C., A. B. Sadosky, and H. A. Shuman. 1994. The Legionella pneumophila icm locus: a set of genes required for intracellular multiplication in human macrophages. Mol. Microbiol. 14: 797-808.
7. Carpenter, P. B., and G. W. Ordal. 1993. Bacillus subtilis FlhA: a flagellar protein related to a new family of signal-transducing receptors. Mol. Microbiol. 7: 735-743.
8. Clemens, D. L., and M. A. Horwitz. 1993. Hypoexpression of major histocompatibility complex molecules on Legionella pneumophila phagosomes and phagolysosomes. Infect. Immun. 61: 2803-2812.
9. Clemens, D. L., and M. A. Horwitz. 1995. Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited. J. Exp. Med. 181: 257-270.
10. d'Enfert, C., I. Reyss, C. Wandersman, and A. P. Pugsley. 1989. Protein secretion by gram-negative bacteria. Characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K-12. J. Biol. Chem. 264:17462-17468.
11. 1 ATPase and to virulence proteins of mammalian and plant pathogens. J. Bacteriol. 175: 3131-3138.
12. 1 ATPase family of proteins. J. Bacteriol. 176: 4501-4510.
13. Elliott, J. A., and W. Johnson. 1981. Immunological and biochemical relationships among flagella isolated from Legionella pneumophila serogroups 1, 2, and 3. Infect. Immun. 33: 602-610.
14. Elliott, J. A., and W. Johnson. 1982. Virulence conversion of Legionella pneumophila serogroup 1 by passage in guinea pigs and embryonated eggs. Infect. Immun. 35: 943-946.
15. Fields, B. S., T. A. Nerad, T. K. Sawyer, C. H. King, J. M. Barbaree, W. T. Martin, W. E. Morrill, and G. N. Sanden. 1990. Characterization of an axenic strain of Hartmannella vermiformis obtained from an investigation of nosocomial legionellosis. J. Protozool. 37: 581-583.
16. Hermant, D., R. Menard, N. Arricau, C. Parsot, and M. Y. Popoff. 1995. Functional conservation of the Salmonella and Shigfflla ctfectors of entry into epithelial cells. Mol. Microbiol. 17: 781-789.
17. Horwitz, M. A. 1983. Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes. J. Exp. Med. 158: 1319-1331.
18. Horwitz, M. A. 1987. Characterization of avirulent mutant Legionella pneumophila that survive but do not multiply within human monocytes. J. Exp. Med. 166: 1310-1328.
19. Horwitz, M. A., and S. C. Silverstein. 1981. Interaction of the Legionnaires' disease bacterium (Legionella pneitmophila) with human phagocytes. II. Antibody promotes binding of L. pneumophila to monocytes but does not inhibit intracellular multiplication. J. Exp. Med. 153: 398-406.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
21. Leong, J. M., R. S. Fournler, and R. R. Isberg. 1990. Identification of the integrin binding domain of the Yersinia pseudotuberculosis invasin protein. EMBO J. 9: 1479-1989.
22. Macnab, R. 1995. GenBank accession no. L49147.
23. Marra, A., S. J. Blander, M. A. Horwitz, und H. A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intrucellulur multipli- cation in human macrophages. Proc. Natl. Acad. Sci. USA 89: 9607-9611.
24. Menard, R., P. J. Sansonetti, and C. Parsot. 1993. Nonpolar mulagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J. Bacteriol. 175: 5899-5906.
25. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio choleras requires toxR. J. Bacteriol. 170: 2575-2583.
26. Müller, A., J. Hacker, and B. C. Brand. 1996. Evidence for apoptosis of human macrophage-like HL-60 cells by Legionella pneumophila infection. Infect. Immun. 64: 4900-4906.
27. Persson, C., R. Nordfelth, A. Holmstrom, S. Hakansson, R. Rosqvist, and W. H. Wolf. 1995. Cell-surface-bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cell. Mol. Microbiol. 18: 135-150.
28. Pruckler, J. M., R. F. Benson, M. Moyenuddin, W. T. Martin, and B. S. Fields. 1995. Association of rlagellum expression and intracellular growth of Legionella pneumophila. Infect. Immun. 63: 4928-4932.
29. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57: 50-108.
30. Rosqvist, R., S. Hakansson, A. Forsberg, and W. H. Wolf. 1995. Functional conservation of the secretion and translocation machinery for virulence proteins of yersiniae, salmonellae and shigellae. EMBO J. 14: 4187-4195.
31. Rowbotham, T. J. 1980. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J. Clin. Pathol. 33: 1179-1183.
32. Sadosky, A. B., L. A. Wiater, and H. A. Shuman. 1993. Identification of Legionella pneumophila genes required for growth within and killing of human macrophages. Infect. Immun. 61: 5361-5373.
33. Salmond, G. P., and P. J. Reeves. 1993. Membrane traffic wardens and protein secretion in gram-negative bacteria. Trends Biochem. Sci. 18: 7-12.
34. Simon, R., M. O'Connell, M. Labes, and A. Punier. 1986. Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria. Methods Enzymol. 118: 640-659.
35. Sory, M. P., A. Boland, I. Lambermont, and G. R. Cornells. 1995. Identification of the YopE and YopH domains required for secretion and internalization into the cytosul of macrophages, using the cyaA gene fusion approach. Proc. Natl. Acad. Sci. USA 92: 11998-12002.
36. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503-517.
37. Swanson, M. S., and R. R. Isberg. 1995. Association of Legionella pneumophila with the macrophage endoplasmic reticulum. Infect. Immun. 63: 3609-3620.
38. Swanson, M. S., and R. R. Isberg. 1996. Identification of Legionella pneumophila mutants that have aberrant intracellular fates. Infect. Immun. 64: 2585-2594.
39. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.
40. Venkatesan, M. M., J. M. Buysse, and E. V. Oaks. 1992. Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus. J. Bacteriol. 174: 1990-2001.
41. 40a. Vogel, J., H. Andrews, and R. Isberg. Unpublished data.
42. 1 vacuolar, and archaebaeterial ATPase subunits. J. Bacteriol. 173: 3564-3572.
43. Walker, J. E., M. Saraste, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1: 945-951.
44. Woestyn, S., A. Allaoui, P. Wattlau, and G. R. Cornells. 1994. YscN, the putative energizer of the Yersinia Yop secretion machinery. J. Bacteriol. 176: 1561-1569.
45. Ying, C. W., F. Scoffone, A. M. Albertini, A. Galizzi, and G. W. Ordal. 1991. Properties of the Bacillus subtilis chemotaxis protein InvJ, homolog of the Salmonella typhimitrium flagellar protein FliJ. J. Bacteriol. 173: 3584-3586.
46. Zierler, M. K., and J. E. Galan. 1995, Contact with cultured epithelial culls stimulates secretion of Salmonella typhitnurhim invasion protein InvJ. Infect. Immun. 63: 4024-4028.