Generation of replication-competent hepatitis B virus nucleocapsids in insect cells

Journal of Virology

Volumn 72, Issue 4, 1998, Pages 2765-2776

  Seifer, Maria ^a   Hamatake, Robert ^a   Bifano, Marc ^a   Standring, David N ^a  

^a BRISTOL MYERS SQUIBB   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; HEPATITIS B VIRUS; NONHUMAN; PRIORITY JOURNAL; VIRUS CELL INTERACTION; VIRUS NUCLEOCAPSID; VIRUS REPLICATION;

EID: 0031925585     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.4.2765-2776.1998     Document Type: Article

References (53)

1. Acs, G., M. A. Sells, R. H. Purcell, P. Price, R. Engle, M. Shapiro, and H. Popper. 1987. Hepatitis B virus produced by transfected Hep G2 cells causes hepatitis in chimpanzees. Proc. Natl. Acad. Sci. USA 84:4641-4644.
2. Bartenschlager, R., M. Junker-Niepmann, and H. Schaller. 1990. The P-gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. J. Virol. 64:5324-5332.
3. Bartenschlager, R., and H. Schaller. 1988. The amino-terminal domain of the hepadnaviral P-gene encodes the terminal protein (genome-linked protein) believed to prime reverse transcription. EMBO J. 7:4185-4192.
4. Bartenschlager, R., and H. Schaller. 1992. Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genome. EMBO J. 11:3413-3420.
5. Beasley, R. P., and L. Y. Hwang. 1991. Overview on the epidemiology of hepatocellular carcinoma, p. 532-535. In F. B. Hollinger, S. M. Lemon, and M. Margolis (ed.), Viral hepatitis and liver disease. Williams and Willkins, Baltimore, Md.
6. Birnbaum, F., and M. Nassal. 1990. Hepatitis B virus nucleocapsid assembly: primary structure requirements in the core protein. J. Virol. 64:3319-3330.
7. Brass, V., and D. Ganem. 1991. The role of the envelope proteins in hepatitis B virus assembly. Proc. Natl. Acad. Sci. USA 88:1059-1063.
8. Chang, L. J., P. Pryciak, D. Ganem, and H. E. Varmus. 1989. Biosynthesis of the reverse transcriptase of hepatitis B viruses involves de novo translational initiation, not ribosomal frameshifting. Nature (London) 337:364-368.
9. Dannaoui, E., C. Trépo, and F. Zoulim. 1997. Inhibitory effect of penciclovirtriphosphate on duck hepatitis B virus reverse transcription. Antiviral Chem. Chemother. 8:38-46.
10. Davis, M. G., J. E. Wilson, N. A. VanDraanen, W. H. Miller, G. A. Freeman, S. M. Daluge, F. L. Boyd, A. E. Aulabaugh, G. R. Painter, and L. R. Boone. 1996. DNA polymerase activity of hepatitis B virus particles: differential inhibition by L-enantiomers of nucleotide analogs. Antiviral Res. 30:133-145.
11. Galibert, F., E. Mandart, F. Fitoussi, P. Tiollais, and P. Charnay. 1979. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646-650.
12. Ganem, D., J. R. Pollack, and J. Tavis. 1994. Hepatitis B virus reverse transcriptase and its many roles in hepadnavirus genomic replication. Infect. Agents Dis. 3:85-93.
13. Gerlich, W., and W. S. Robinson. 1980. Hepatitis B virus contains a protein attached to the 5′ terminus of its complete DNA strand. Cell 21:801-809.
14. Hatton, T., S. Zhou, and D. N. Standring. 1992. RNA- and DNA-binding activities in hepatitis B virus capsid protein: a model for their roles in viral replication. J. Virol. 66:5232-5241.
15. Hilditch, C. M., L. J. Rogers, and D. H. L. Bishop. 1990. Physiochemical analysis of the hepatitis core antigen produced by a baculovirus expression vector. J. Gen. Virol. 71:2755-2759.
16. Hirsch, R. C., J. E. Lavine, L. J. Chang, H. E. Varmus, and D. Ganem. 1990. Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature (London) 344: 552-555.
17. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
18. Lanford, R. E., and L. Notvall. 1990. Expression of hepatitis B virus core and precore antigens in insect cells and characterisation of a core-associated kinase activity. Virology 176:222-233.
19. Lanford, R. E., L. Notvall, and B. Beames. 1995. Nucleotide priming and reverse transcriptase activity of hepatitis B virus polymerase expressed in insect cells. J. Virol. 69:4431-4439.
20. Lanford, R. E., L. Notvall, H. Lee, and B. Beames. 1997. Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase. J. Virol. 71:2995-3004.
21. Loeb, D. D., and R. Tian. 1995. Transfer of the minus strand of DNA during hepadnavirus replication is not invariant but prefers a specific location. J. Virol. 69:6886-6891.
22. Machida, A., H. Ohnuma, F. Tsuda, A. Yoshikawa, Y. Hoshi, T. Tanaka, S. Kishimoto, Y. Akahane, Y. Miyakawa, and M. Mayumi. 1991. Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibody. J. Virol. 65:6024-6030.
23. Miller, R., P. Marion, and W. Robinson. 1984. Hepatitis B viral DNA-RNA hybrid molecules in particles from infected liver are converted to viral DNA molecules during an endogenous DNA polymerase reaction. Virology 139: 64-72.
24. Molnar-Kimber, K. L., J. Summers, J. M. Taylor, and W. S. Mason. 1983. Protein covalently bound to minus-strand DNA intermediates of duck hepatitis B virus. J. Virol. 45:165-172.
25. Nassal, M. 1992. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. J. Virol. 66:4107-4116.
26. Nassal, M., and A. Rieger. 1996. A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis. J. Virol. 70:2764-2773.
27. Oberhaus, S. M., and J. E. Newbold. 1993. Detection of DNA polymerase activities associated with purified duck hepatitis B virus core particles by using an activity gel assay. J. Virol. 67:6558-6566.
28. O'Reilly, D. R., L. K. Miller, and V. A. Luckow. 1994. Baculovirus expression vectors: a laboratory manual. Oxford University Press, New York, N.Y.
29. Radziwill, G., W. Tucker, and H. Schaller. 1990. Mutational analysis of the hepatitis B virus P gene product: domain structure and RNase H activity. J Virol. 64:613-620.
30. Radziwill, G., H. Zentgraf, H. Schaller, and V. Bosch. 1988. The duck hepatitis B virus DNA polymerase is tightly associated with the viral core structure and unable to switch to an exogenous template. Virology 163:123-132.
31. Rieger, A., and M. Nassal. 1996. Specific hepatitis B virus minus-strand DNA synthesis requires only the 5′ encapsidation signal and the 3′-proximal direct repeat DR1*. J. Virol. 70:585-589.
32. Rodgers, D. W., S. J. Gamblin, B. A. Harris, S. Ray, J. S. Culp, B. Hellmig, D. J. Woolf, C. Debouck, and S. C. Harrison. 1995. The structure of the unliganded reverse transcriptase from the human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 92:1222-1226.
33. Schlicht, H. J., R. Bartenschlager, and H. Schaller. 1989. The duck hepatitis B virus core protein contains a highly phosphorylated C terminus that is essential for replication but not for RNA packaging. J. Virol. 63:2995-3000.
34. Schlicht, H. J., G. Radziwill, and H. Schaller. 1989. Synthesis and encapsidation of duck hepatitis B virus reverse transcriptase do not require formation of core-polymerase fusion proteins. Cell 56:85-92.
35. Seeger, C., J. Summers, and W. S. Mason. 1991. Viral DNA synthesis. Curr. Top. Microbiol. Immunol. 168:41-59.
36. Seifer, M., and D. N. Standring. Unpublished observations.
37. Seifer, M., and D. N. Standring. 1993. Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the viral nucleocapsid or the replication origin, DR1. J. Virol. 67:4513-4520.
38. Seifer, M., and D. N. Standring. 1995. Ribonucleoprotein complex formation by the human hepatitis B virus polymerase. Intervirology 38:295-303.
39. Sells, M. A., M.-L. Chen, and G. Acs. 1987. Production of hepatitis B virus particles in HepG2 cells transfected with cloned hepatitis B virus DNA. Proc. Natl. Acad. Sci. USA 84:1005-1009.
40. Staprans, S., D. D. Loeb, and D. Ganem. 1991. Mutations affecting hepadnaviral plus-strand DNA synthesis dissociate primer cleavage from translocation and reveal the origin of linear viral DNA. J. Virol. 65:1255-1262.
41. Summers, J., and W. S. Mason. 1982. Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. Cell 29:403-415.
42. Takahashi, K., S. Kishimoto, K. Ohori, H. Yoshizawa, A. Machida, H. Ohnuma, F. Tsuda, E. Munekata, Y. Miyakawa, and M. Mayumi. 1991. Molecular heterogeneity of e antigen polypeptides in sera from carriers of hepatitis B virus. J. Immunol. 147:3156-3160.
43. Takahashi, K., A. Machida, G. Funatsu, M. Nomura, S. Usuda, S. Aoyagi, K. Tachibana, Y. Miyakawa, and M. Mayumi. 1983. Immunochemical structure of hepatitis B antigen in the serum. J. Immunol. 130:2903-2907.
44. Tavis, J. E., and D. Ganem. 1996. Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template. J. Virol. 70:5741-5750.
45. Tavis, J. E., and D. Ganem. 1993. Expression of functional hepatitis B virus polymerase in yeast reveals it to be the sole viral protein required for correct initiation of reverse transcription. Proc. Natl. Acad. Sci. USA 90:4107-4111.
46. Tavis, J. E., and D. Ganem. 1995. RNA sequences controlling the initiation and transfer of duck hepatitis B virus minus-strand DNA. J. Virol. 69:4283-4291.
47. Tavis, J. E., S. Perri, and D. E. Ganem. 1994. Hepadnaviral reverse transcription initiates within a stem-loop of the RNA packaging signal and employs a novel strand transfer. J. Virol. 68:3536-3543.
48. Valenzuela, P. Personal communication.
49. Wang, G. H., and C. Seeger. 1993. Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67:6507-6512.
50. Wang, G. H., and C. Seeger. 1992. The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesis. Cell 71:663-670.
51. Wu, T.-T., L. D. Condreay, L. Coates, C. Aldrich, and W. Mason. 1991. Evidence that less-than-full-length pol gene products are functional in hepadnavirus DNA synthesis. J. Virol. 65:2155-2163.
52. Yu, M., and J. Summers. 1991. A domain of the hepadnavirus capsid protein is specifically required for DNA maturation and virus assembly. J. Virol. 65: 2511-2517.
53. Zhou, S., and D. N. Standring. 1991. Production of hepatitis B virus nucleocapsidlike core particles in Xenopus oocytes: assembly occurs mainly in the cytoplasm and does not require the nucleus. J. Virol. 65:5457-5464.