Separation of hybridoma cells from their IgG product using aqueous two- phase systems

Bioseparation

Volumn 6, Issue 4, 1996, Pages 201-210

  Zijlstra, G M ^a  

^a Bio Intermediair Europe BV   (Netherlands)

Author keywords

Aqueous Two phase Systems; Dextran; Dye Ligand; Extractive Bioconversion; Full Factorial Design; IgG; Partitioning; Poly(ethylene glycol)

Indexed keywords

ANTIBODIES; BIOCONVERSION; BIOTECHNOLOGY; CELLS; MOLECULAR WEIGHT; PH; POLYETHYLENE GLYCOLS; POTASSIUM COMPOUNDS;

AQUEOUS TWO PHASE SYSTEM; DEXTRAN; DYE LIGAND; EXTRACTIVE BIOCONVERSION; HYBRIDOMA CELL; PARTITIONING; TIE LINE LENGTH;

PHASE SEPARATION;

IMMUNOGLOBULIN G; INORGANIC SALT; LIGAND; WATER;

ARTICLE; CHEMISTRY; HYBRIDOMA; ISOLATION AND PURIFICATION; PH;

HYBRIDOMAS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; LIGANDS; SALTS; WATER;

EID: 0030436455     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (3)

1. Abbott N, Blankschtein D and Hatton A (1990) On protein partitioning in two-phase aqueous polymer systems. Bioseparation 1: 191-225.
2. Albertsson PA (1986) In Partitioning of Cell Particles and Macromolecules. 3rd edition. Wiley & Sons, New York.
3. Andersson E and Hahn-Hägerdal B (1990) Bioconversions in aqueous two-phase systems. Enzyme Microb. Technol. 12: 242-253.