In vitro activity of hepatitis B virus polymerase: Requirement for distinct metal ions and the viral epsilon stem-loop

Journal of General Virology

Volumn 79, Issue 5, 1998, Pages 1121-1131

  Urban, Margit ^a,c   McMillan, David J ^a   Canning, Gail ^a   Newell, Anne ^a,b   Brown, Emma ^a   Mills, John S ^a,d   Jupp, Ray ^a,b  

^a ROCHE PRODUCTS LTD   (United Kingdom)

^b SANOFI   (France)

^c MORPHOSYS AG   (Germany)

^d UCB PHARMA   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOTIDE; METAL ION; RNA DIRECTED DNA POLYMERASE; TYROSINE; VIRUS ENZYME; VIRUS RNA;

ARTICLE; BACULOVIRUS; DNA VIRUS; ENZYME PURIFICATION; HEPATITIS B VIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; RNA TRANSCRIPTION; VIRUS REPLICATION;

DNA VIRUSES; HEPATITIS B VIRUS; UNIDENTIFIED BACULOVIRUS; UNIDENTIFIED RETROVIRUS;

EID: 0031922339     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-79-5-1121     Document Type: Article

References (36)

1. Ayola, B., Kanda, P. & Lanford, R. E. (1993). High level expression and phosphorylation of hepatitis B virus polymerase in insect cells with recombinant baculoviruses. Virology 194, 370-373.
2. Bartenschlager, R., Junker-Niepmann, M. & Schaller, H. (1990). The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. Journal of Virology 64, 5324-5332.
3. Bartenschlager, R., Kuhn, C. & Schaller, H. (1992), Expression of the P-protein of the human hepatitis B virus in a vaccinia virus system and detection of the nucleocapsid-associated P-gene product by radiolabelling at newly introduced phosphorylation sites. Nucleic Acids Research 20, 195-202.
4. Bavand, M., Feitelson, M. & Laub, O. (1989). The hepatitis B virus-associated reverse transcriptase is encoded by the viral pol gene. Journal of Virology 63, 1019-1021.
5. Black, C. B. & Cowan, J. A. (1995). Magnesium-dependent enzymes in nucleic acid biochemistry. In The Biological Chemistry of Magnesium, pp. 139-158. Edited by J. A. Cowan. New York: VCH.
6. Caldentey, J., Blanco, L., Savilahti, H., Bamford, D. H. & Salas, M. (1992). In vitro replication of bacteriophage PRD1 DNA. Metal activation of protein-primed initiation and DNA elongation. Nucleic Acids Research 20, 3971-3976.
7. Esteban, J. A., Bernad, A., Salas, M. & Blanco, L. (1992). Metal activation of synthetic and degradative activities of phi 29 DNA polymerase, a model enzyme for protein-primed DNA replication. Biochemistry 31, 350-359.
8. Galibert, F., Mandart, E., Fitoussi, F., Tiollais, P. & Charnay, P. (1979). Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281, 646-650.
9. Ganem, D. (1996). Hepadnaviridae: the viruses and their replication. In Fields Virology, 3rd edn, pp. 2703-2737. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott-Raven.
10. Hirsch, R. C., Lavine, J. E., Chang, L. J., Varmus, H. E. & Ganem, D. (1990). Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcription. Nature 344, 552-555.
11. Hollinger, F. B. (1996). Hepatitis B virus. In Fields Virology, 3rd edn, pp. 2739-2807. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott-Raven.
12. Hoofnagle, J. H. & Lau, D. (1997), New therapies for chronic hepatitis B. Journal of Viral Hepatitis 4(Suppl. 1), 41-50.
13. Hu, J. & Seeger, C. (1996). Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase. Proceedings of the National Academy of Sciences, USA 93, 1060-1064.
14. Junker, M., Galle, P. & Schaller, H. (1987). Expression and replication of the hepatitis B virus genome under foreign promoter control. Nucleic Acids Research 15, 10117-10132.
15. Junker-Niepmann, M., Bartenschlager, R. & Schaller, H. (1990). A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO Journal 9, 3389-3396.
16. Lanford, R. E., Notvall, L. & Beames, B. (1995). Nucleotide priming and reverse transcriptase activity of hepatitis B virus polymerase expressed in insect cells. Journal of Virology 69, 4431-4439.
17. Lanford, R. E., Notvall, L., Lee, H. & Beames, B. (1997). Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase. Journal of Virology 71, 2996-3004.
18. Leith, I. R., Hay, R. T. & Russell, W. C. (1989). Adenovirus subviral particles and cores can support limited DNA replication. Journal of General Virology 70, 3235-3248.
19. Lok, A. S. (1994). Treatment of chronic hepatitis B. Journal of Viral Hepatitis 1, 105-124.
20. Mason, W. S., Lien, J., Petcu, D. L., Coates, L., London, W. T., O'Connel, A., Aldrich, C. & Custer, R. P. (1987). In vivo and in vitro studies on duck hepatitis B virus replication. In Hepadnaviruses, pp. 3-16. Edited by W. S. Robinson, K. Koike & H. Will. New York: A. R. Liss.
21. Nassal, M. & Schaller, H. (1996). Hepatitis B virus replication - an update. Journal of Viral Hepatitis 3, 217-226.
22. Prasad, V. R., Lowy, I., de los Santos, T., Chiang, L. & Goff, S. P. (1991). Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase. Proceedings of the National Academy of Sciences, USA 88, 11363-11367.
23. Pronk, R., Van Driel, W. & Van der Vliet, P. C. (1994). Replication of adenovirus DNA in vitro is ATP-independent. FEBS Letters 337, 33-38.
24. Radziwill, G., Tucker, W. & Schaller, H. (1990). Mutational analysis of the hepatitis B virus P gene product: domain structure and RNase H activity. Journal of Virology 64, 613-620.
25. Rieger, A. & Nassal, M. (1996). Specific hepatitis B virus minus-strand DNA synthesis requires only the 5′ encapsidation signal and the 3′-proximal direct repeat DR1. Journal of Virology 70, 585-589.
26. Salas, M. (1991). Protein-priming of DNA replication. Annual Review of Biochemistry 60, 39-71.
27. Schalm, S. W., de Man, R. A., Heijtink, R. A. & Niesters, H. G. (1995). New nucleoside analogues for chronic hepatitis B. Journal of Hepatology 22, 52-56.
28. Seifer, M. & Standring, D. N. (1993). Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1. Journal of Virology 67, 4513-4520.
29. Shin, H. J. & Rho, H. M. (1995). Release of the hepatitis B virus-associated DNA polymerase from the viral particle by the proteolytic cleavage. Journal of Biological Chemistry 270, 11047-11050.
30. Stueber, D., Matile, H. & Garotta, G. (1990). System for high-level production in Escherichia coli and rapid purification of recombinant proteins: application to epitope mapping, preparation of antibodies, and structure-function analysis. In Immunological Methods, pp. 121-152. Edited by I. Lefkovits & B. Pernis. New York: Academic Press.
31. Summers, M. D. & Smith, G. E. (1987). A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin no. 1555, pp. 1-48.
32. Tavis, J. E. & Ganem, D. (1993). Expression of functional hepatitis B virus polymerase in yeast reveals it to be the sole viral protein required for correct initiation of reverse transcription. Proceedings of the National Academy of Sciences, USA 90, 4107-4111.
33. Tavis, J. E. & Ganem, D. (1996). Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template. Journal of Virology 70, 5741-5750.
34. Wang, G. H. & Seeger, C. (1992). The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesis. Cell 71, 663-670.
35. Wang, G. H., Zoulim, F., Leber, E. H., Kitson, J. & Seeger, C. (1994). Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B viruses. Journal of Virology 68, 8437-8442.
36. Woo, M. H. & Burnakis, T. G. (1997). Interferon alfa in the treatment of chronic viral hepatitis B and C. Annual Pharmacotherapy 31, 330-337.