Comparison of two methods of processing induced sputum: Selected versus entire sputum

American Journal of Respiratory and Critical Care Medicine

Volumn 157, Issue 2, 1998, Pages 665-668

  Spanevello, Antonio ^a   Beghé, Bianca ^b   Bianchi, Achille ^b   Migliori, Giovanni Battista ^b   Ambrosetti, Marco ^b   Neri, Margherita ^b   Ind, Philip W ^b  

^a IRCCS   (Italy)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

EOSINOPHIL CATIONIC PROTEIN;

ADULT; ARTICLE; ASTHMA; BRONCHOSPASM; CLINICAL ARTICLE; COMPARATIVE STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DISEASE SEVERITY; EOSINOPHILIA; FEMALE; HUMAN; LEUKOCYTE COUNT; MALE; PRIORITY JOURNAL; PROTEIN DETERMINATION; SPUTUM ANALYSIS; SPUTUM CYTODIAGNOSIS;

EID: 0031911398     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/ajrccm.157.2.9705095     Document Type: Article

References (16)

1. Pin, I., P. G. Gibson, R. Kolendovicz, A. Girgis-Gabardo, J. Denburg, F. E. Hargreave, and J. Dolovich. 1992. Use of induced sputum cell counts to investigate airway inflammation in asthma. Thorax 47:25-29.
2. Popov, T., M. M. M. Pizzichini, E. Pizzichini, R. Kolendowicz, Z. Punthakee, J. Dolovich, and F. E. Hargreave. 1995. Some technical factors influencing the induction of sputum for cell analysis. Eur. Respir. J. 8:559-565.
3. Fahy, J. V., J. Liu, H. Wong, and H. A. Boushey. 1993. Cellular and biochemical analysis of induced sputum from asthmatic and from healthy subjects. Am. Rev. Respir. Dis. 147:1126-1131.
4. Pin, I., A. P. Freitag, P. M. O'Byrne, A. Girgis-Gabardo, R. M. Watson, J. Dolovich, J. A. Denburg, and F. E. Hargreave. 1992. Changes in the cellular profile of induced sputum after allergen-induced asthmatic responses. Am. Rev. Respir. Dis. 145:1265-1269.
5. Claman, D. M., H. A. Boushey, J. Liu, H. Wong, and J. V. Fahy. 1994. Analysis of induced sputum to examine the effects of prednisone on airway inflammation. J. Allergy Clin. Immunol. 94:861-869.
6. Fahy, J. V., H. Wong, J. Liu, and H. A. Boushey. 1994. Analysis of cellular and biochemical constituents of induced sputum after allergen challenge: a method for studying allergic airway inflammation. J. Allergy Clin. Immunol. 93:1031-1039.
7. Pizzichini, E., M. M. M. Pizzichini, A. Efthimiadis, S. Evans, M. M. Morris, D. Squillace, G. J. Gleich, J. Dolovich, and F. E. Hargreave. 1996. Indices of airway inflammation in induced sputum: reproducibility and validity of cell fluid-phase measurements. Am. J. Respir. Crit. Care Med. 154:308-317.
8. Spanevello, A., G. B. Migliori, A. M. Sharara, L. Ballardini, P. Bridge, P. Pisati, M. Neri, and P. W. Ind. 1997. Induced sputum to assess airway inflammation: a study of reproducibility. Clin. Exp. Allergy (In press)
9. In't Veen, J. C. C. M., H. W. F. M. De Gouw, H. H. Smits, J. K. Sont, P. S. Hiemstra, P. J. Sterk, and E. H. Bel. 1996. Repeatibility of cellular and soluble markers of inflammation in induced sputum from patients with asthma. Eur. Respir. J. 9:2441-2447.
10. Ouanjer, P. H., G. J. Tammeling, J. E. Cotes, R. Pedersen, R. Peslin, and J. C. Yernault. 1993. Lung volumes and forced ventilatroy flows: report of working party, standardization of lung function tests. European Community for Steel and Coal. Eur. Respir. J. 6(Suppl. 16):5-40.
11. National Heart, Lung, and Blood Institute. 1995. Global strategy for asthma management and prevention. National Institutes of Health, Bethesda, MD. NHLBI Publication No. 95-3659.
12. Iredale, M. J., S. A. R. Wanklin, I. P. Phillips, T. Krausz, and P. W. Ind. 1994. Noninvasive assessment of bronchial inflammation in asthma: no correlation between eosinophilia of induced sputum and bronchial responsiveness to inhaled hypertonic saline. Clin. Exp. Allergy 24:940-945.
13. Bland, J. M., and D. G. Altman. 1986. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet i:307-310.
14. Pizzichini, E., M. M. M. Pizzichini, A. Ffthimiadis, F. E. Hargreave, and J. Dolovich. 1996. Measurement of inflammatory indices in induced sputum: effects of selection of sputum to minimize salivary contamination. Eur. Respir. J. 9:1174-1180.
15. Hansel, T. T. 1994. The cardinal importance of sputum microscopy. Clin. Exp. Allergy 24:695-697.
16. Tang, C. S., I. T. M. Kung. 1993. Homogenization of sputum with dithiothreitol for early diagnosis of pulmonary malignancies. Acta Cytol. 37:689-693.