Cellular uptake properties of oligonucleotides in LLC-PK1 renal epithelial cells

Antisense and Nucleic Acid Drug Development

Volumn 8, Issue 1, 1998, Pages 67-73

  Takakura, Yoshinobu ^a   Oka, Yoshikazu ^a   Hashida, Mitsuru ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROQUINE; FLUORESCEIN ISOTHIOCYANATE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; OLIGONUCLEOTIDE; POLYCARBONATE; SODIUM AZIDE; SULFUR 35;

ANIMAL CELL; APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; CONTROLLED STUDY; ENDOCYTOSIS; KIDNEY PROXIMAL TUBULE; LASER MICROSCOPY; LYSOSOME; MICHAELIS MENTEN KINETICS; MONOLAYER CULTURE; NONHUMAN; NUCLEIC ACID TRANSPORT; PRIORITY JOURNAL; TEMPERATURE; TIME;

EID: 0031888730     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1998.8.67     Document Type: Article

References (6)

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2. AKHTAR, S., BECK, G.F., HAWLEY, P., IRWIN, W.J., and GIBSON, I. (1996). The influence of polarized epithelial (Caco-2) cell differentiation on the cellular binding of phosphodiester and phosphorothioate oligonucleotides. Antisense Nucleic Acid Drug Dev. 6, 197-206.
3. BECK, G.F., IRWIN, W.J., NICKLIN, P.L., and AKHTAR, S. (1996). Interactions of phosphodiester and phosphorothioate oligonucleotides with intestinal epithelial Caco-2 cells. Pharm. Res. 13, 1028-1037.
4. BENIMETSKAYA, L., LOIKE, J.D., KHALED, Z., LOIKE, G., SILVERSTEIN, S.C., CAO, L., KHOURY, J.E., CAI, T.-Q., and STEIN, C.A. (1997). Mac-1 (CD11b/CD18) is an oligodeoxynucleotide-binding protein. Nature Med. 3, 414-420.
5. BISHOP, M.R., IVERSEN, P.L., BAYEVER, E., SHARP, J.G., GREINER, T.C., COPPLE, B.L., RUDDON, R., ZON, G., ARNESON, M., ARMITAGE, J.O., and KESSINGER, A. (1996). Phase I trial of antisense oligonucleotide OL(1)p53 in hematologic malignancies. J. Clin. Oncol. 14, 1320-1326.
6. ZHAO, Q., MATSON, S., HERRERA, C.J., FISHER, E., YU, H., and KRIEG, A.M. (1993). Comparison of cellular binding and uptake of antisense phosphodiester, phosphorothioate, and mixed phosphorothioate and methylphosphonate oligonucleotides. Antisense Res. Dev. 3, 53-66.