The influence of polarized epithelial (Caco-2) cell differentiation on the cellular binding of phosphodiester and phosphorothioate oligonucleotides

Antisense and Nucleic Acid Drug Development

Volumn 6, Issue 3, 1996, Pages 197-206

  Akhtar, S ^a   Beck, G F ^a   Hawley, P ^b   Irwin, W J ^a   Gibson, I ^b  

^a ASTON UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; OLIGONUCLEOTIDE;

ARTICLE; CELL AGING; CELL DIFFERENTIATION; DRUG BINDING; DRUG UPTAKE; EPITHELIUM CELL; PH; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE;

TRIXIS;

EID: 0029806868     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1996.6.197     Document Type: Article

References (6)

1. AGRAWAL, S., ZHANG, X., LU, Z., ZHAO, H., TAMBURIN, J.M., YAN, J., CAI, H., DIASLO, R.D., HABUS, I., JIANG, Z., IYER, R.P., YU, D., and ZHANG, R., (1995). Absorption, tissue distribution and in vivo stability in rats of a hybrid antisense oligonucleotide following oral administration. Biochem. Pharmacol. 50, 571-576.
2. AKHTAR, S. (1995). Delivery Strategies for Antisense Oligonucleotide Therapeutics. (CRC Press, Boca Raton, FL).
3. AKHTAR, S., BASU, S., WICKSTROM, E., and JULIANO, R.L. (1991). Interactions of antisense DNA oligonucleotide analogs with phospholipid membranes (liposomes). Nucleic Acids Res. 19, 5551-5559.
4. AKHTAR, S., and JULIANO, R.L. (1992). Liposome delivery of antisense oligodeoxynucleotides: Adsorption and efflux characteristics of phosphorothioates. J. Controlled Release 22, 47-56.
5. BECK, G.F., IRWIN, W.J., NICKLIN, P.L., and AKHTAR, S. (1996). Interactions of phosphodiester and phosphorothioate oligonucleotides with intestinal epithelial Caco-2 cells. Pharm. Res. In press.
6. ZHAO, Q., MATSON, S., HERRERA, C.J., FISHER, E., YU, H., and KRIEG, A.M. (1993). Comparison of cellular binding and uptake of antisense phosphodiester, phosphorothioate, and mixed phosphorothioate and methylphosphonate oligonucleotides. Antisense Res. Dev. 3, 53-66.