A subtractive PCR-based cDNA library made from fetal thymic stromal cells

Journal of Immunological Methods

Volumn 213, Issue 2, 1998, Pages 169-182

  Kim, Moon G ^a   Chen, Chuan ^a   Flomerfelt, Francis A ^a   Germain, Ronald N ^a   Schwartz, Ronald H ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

CDNA library; Fetal thymic organ culture; Polymerase chain reaction; Subtractive hybridization; Thymic stromal cells

Indexed keywords

COMPLEMENTARY DNA;

ANIMAL TISSUE; ARTICLE; DNA LIBRARY; DNA SEQUENCE; FETUS; GENE EXPRESSION; MOUSE; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCID MOUSE; STROMA CELL; THYMUS;

ANIMALS; BASE SEQUENCE; BLOTTING, NORTHERN; DEOXYGUANOSINE; DNA, COMPLEMENTARY; FEMALE; FETUS; GENE EXPRESSION; GENES; MALE; MICE; MICE, INBRED C57BL; MICE, SCID; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; ORGAN CULTURE TECHNIQUES; POLYMERASE CHAIN REACTION; RNA, MESSENGER; STROMAL CELLS; THYMUS GLAND;

EID: 0031856890     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00031-3     Document Type: Article

References (39)

1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403.
2. Anderson G., Anderson K.L., Conroy L.A., Hallam T.J., Moore N.C., Owen J.J., Jenkinson E.J. Intracellular signaling events during positive and negative selection of CD4+CD8+ thymocytes in vitro. J. Immunol. 154:1995;3636.
3. Anderson G., Anderson K.L., Tchilian E.Z., Owen J.J., Jenkinson E.J. Fibroblast dependency during early thymocyte development maps to the CD25+ CD44+ stage and involves interactions with fibroblast matrix molecules. Eur. J. Immunol. 27:1997;1200.
4. Anderson G., Jenkinson E.J. The role of the thymus during T-lymphocyte development in vitro. Semin. Immunol. 7:1995;177.
5. Anderson G., Jenkinson E.J., Moore N.C., Owen J.J. MHC class II-positive epithelium and mesenchyme cells are both required for T-cell development in the thymus. Nature. 362:1993;70.
6. Anderson G., Moore N.C., Owen J.J., Jenkinson E.J. Cellular interactions in thymocyte development. Annu. Rev. Immunol. 14:1996;73.
7. Anderson G., Owen J.J., Moore N.C., Jenkinson E.J. Thymic epithelial cells provide unique signals for positive selection of CD4+CD8+ thymocytes in vitro. J. Exp. Med. 179:1994;2027.
8. Anderson K.L., Anderson G., Michell R.H., Jenkinson E.J., Owen J.J. Intracellular signaling pathways involved in the induction of apoptosis in immature thymic T lymphocytes. J. Immunol. 156:1996;4083.
9. Belyavsky, A., Vinogradova, T., Rajewsky, K., 1989. PCR-based cDNA library construction: general cDNA libraries at the level of a few cells [published erratum appears in Nucleic Acids Res., 1989, July 25; 17 (14) 5883]. Nucleic Acids Res., 17, 2919.
10. Boyd R.L., Hugo P. Towards an integrated view of thymopoiesis. Immunol. Today. 12:1991;71.
11. Boyd R.L., Tucek C.L., Godfrey D.I., Izon D.J., Wilson T.J., Davidson N.J., Bean A.G., Ladyman H.M., Ritter M.A., Hugo P. The thymic microenvironment. Immunol. Today. 14:1993;445-459.
12. Burkly L.C., Degermann S., Longley J., Hagman J., Brinster R.L., Lo D., Flavell R.A. Clonal deletion of V beta 5+ T cells by transgenic I-E restricted to thymic medullary epithelium. J. Immunol. 151:1993;3954.
13. Faas S.J., Rothstein J.L., Kreider B.L., Rovera G., Knowles B.B. Phenotypically diverse mouse thymic stromal cell lines which induce proliferation and differentiation of hematopoietic cells. Eur. J. Immunol. 23:1993;1201.
14. Gurr, S.J., McPherson, M. (1991). PCR-directed cDNA libraries. In: McPherson, J., Quirke, P., Taylor, G.R. (Eds.), PCR A practical approach. IRL Press, New York, p. 147.
15. Izon D.J., Jones L.A., Eynon E.E., Kruisbeek A.M. A molecule expressed on accessory cells, activated T cells, and thymic epithelium is a marker and promoter of T cell activation. J. Immunol. 153:1994;2939.
16. Jenkinson E.J., Anderson G. Fetal thymic organ cultures. Curr. Opin. Immunol. 6:1994;293.
17. Jenkinson E.J., Anderson G., Owen J.J. Studies on T cell maturation on defined thymic stromal cell populations in vitro. J. Exp. Med. 176:1992;845.
18. Jenkinson E.J., Franchi L.L., Kingston R., Owen J.J. Effect of deoxyguanosine on lymphopoiesis in the developing thymus rudiment in vitro: application in the production of chimeric thymus rudiments. Eur. J. Immunol. 12:1982;583.
19. Jenkinson E.J., Owen J.J. T-cell differentiation in thymus organ cultures. Semin. Immunol. 2:1990;51.
20. Kampinga J., Berges S., Boyd R.L., Brekelmans P., Colic M., van Ewijk W., Kendall M.D., Ladyman H., Nieuwenhuis P., Ritter M.A.et al. Thymic epithelial antibodies: immunohistological analysis and introduction of nomenclature. Thymus. 13:1989;165.
21. Laufer T.M., DeKoning J., Markowitz J.S., Lo D., Glimcher L.H. Unopposed positive selection and autoreactivity in mice expressing class II MHC only on thymic cortex. Nature. 383:1996;81.
22. Liang P., Pardee A.B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science. 257:1992;967.
23. Lisitsyn N., Lisitsyn N., Wigler M. Cloning the differences between two complex genomes. Science. 259:1993;946.
24. Nehls M., Kyewski B., Messerle M., Waldschutz R., Schuddekopf K., Smith A.J., Boehm T. Two genetically separable steps in the differentiation of thymic epithelium. Science. 272:1996;886.
25. Nehls M., Pfeifer D., Schorpp M., Hedrich H., Boehm T. New member of the winged-helix protein family disrupted in mouse and rat nude mutations. Nature. 372:1994;103.
26. Palmer D.B., George A.J., Ritter M.A. Selection of antibodies to cell surface determinants on mouse thymic epithelial cells using a phage display library. Immunology. 91:1997;473.
27. Rocha D., Carrier A., Naspetti M., Victorero G., Anderson E., Botcherby M., Guenet J.L., Nguyen C., Naquet P., Jordan B.R. Modulation of mRNA levels in the presence of thymocytes and genome mapping for a set of genes expressed in mouse thymic epithelial cells. Immunogenetics. 46:1997;142.
28. Ronchese F., Schwartz R.H., Germain R.N. Functionally distinct subsites on a class II major histocompatibility complex molecule. Nature. 329:1987;254.
29. Shores E.W., Van Ewijk W., Singer A. Disorganization and restoration of thymic medullary epithelial cells in T cell receptor-negative scid mice: evidence that receptor-bearing lymphocytes influence maturation of the thymic microenvironment. Eur. J. Immunol. 21:1991;1657.
30. Tucek C.L., Godfrey D.I., Boyd R.L. Five novel antigens illustrate shared phenotype between mouse thymic stromal cells, thymocytes, and peripheral lymphocytes. Int. Immunol. 4:1992;1021.
31. van Ewijk W. T-cell differentiation is influenced by thymic microenvironments. Annu. Rev. Immunol. 9:1991;591.
32. Van Ewijk W., de Kruif J., Germeraad W.T., Berendes P., Ropke C., Platenburg P.P., Logtenberg T. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA. 94:1997;3903.
33. van Ewijk W., Ron Y., Monaco J., Kappler J., Marrack P., Le Meur M., Gerlinger P., Durand B., Benoist C., Mathis D. Compartmentalization of MHC class II gene expression in transgenic mice. Cell. 53:1988;357.
34. van Vliet E., Melis M., van Ewijk W. Monoclonal antibodies to stromal cell types of the mouse thymus. Eur. J. Immunol. 14:1984;524.
35. Vieira J.L., de Souza L.R., Savino W. Conceptual aspects of the thymic microenvironment. Immunol. Today. 15:1994;192. Letter; comment.
36. Vukmanovic S., Grandea A.G.d., Faas S.J., Knowles B.B., Bevan M.J. Positive selection of T-lymphocytes induced by intrathymic injection of a thymic epithelial cell line. Nature. 359:1992;729.
37. Vukmanovic S., Jameson S.C., Bevan M.J. A thymic epithelial cell line induces both positive and negative selection in the thymus. Int. Immunol. 6:1994;239.
38. Vukmanovic S., Stella G., King P.D., Dyall R., Hogquist K.A., Harty J.T., Nikolic-Zugic J., Bevan M.J. A positively selecting thymic epithelial cell line lacks costimulatory activity. J. Immunol. 152:1994;3814.
39. Welsh J., Liu J.P., Efstratiadis A. Cloning of PCR-amplified total cDNA: construction of a mouse oocyte cDNA library. Genet. Anal. Tech. Appl. 7:1990;5.