Escherichia coli O157 serology: False-positive ELISA results caused by human antibodies binding to bovine serum albumin

Letters in Applied Microbiology

Volumn 27, Issue 2, 1998, Pages 76-78

  Chart, H ^a   Evans, J ^a   Chalmers, R M ^b   Salmon, R L ^a  

^a CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

^b CDSC Wales   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; ESCHERICHIA COLI; FARMS; MAMMALS; SURFACE PLASMON RESONANCE;

ANTIBODY BINDING; BACKGROUND LEVEL; BOVINE SERUM ALBUMINS; ESCHERICHIA COLI O157; FALSE POSITIVE; FARM WORKERS; HUMAN ANTIBODIES; IMMUNOBLOTTING; LIPOPOLYSACCHARIDES;

ANTIBODIES;

BOVINE SERUM ALBUMIN; LIPOPOLYSACCHARIDE;

AGRICULTURAL WORKER; ANTIBODY BLOOD LEVEL; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC ERROR; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI O157; HUMAN; IMMUNOBLOTTING; MAJOR CLINICAL STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABBIT; SERODIAGNOSIS;

EID: 0031856192     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1998.00396.x     Document Type: Article

References (27)

1. Chapman, P.A. and Siddons, C.A. (1996) A comparison of immunomagnetic separation and direct culture for the isolation of verotoxin-producing Escherichia coli O157 from cases of bloody diarrhoea, non-bloody diarrhoea and asymptomatic contacts. Journal of Medical Microbiology 44, 267-271.
2. Chapman, P.A., Siddons, C.A., Wright, D.J., Norman, P., Fox, J. and Crick, E. (1992) Cattle as a source of verotoxigenic Escherichia coli O157. Veterinary Record 131, 323-324.
3. Chapman, P.A., Siddons, C.A., Wright, D.J., Norman, P., Fox, J. and Crick, E. (1993a) Cattle as a possible source of verotoxin-producing Escherichia coli O157 infections in man. Epidemiology and Infection 111, 439-447.
4. Chapman, P.A., Wright, D.J. and Higgins, R. (1993b) Untreated milk as a source of verotoxigenic E. coli. O157. Veterinary Record 133, 171-172.
5. Chapman, P.A., Wright, D.J. and Norman, P. (1989) Verotoxin-producing Escherichia coli in Sheffield : cattle as a possible source. Epidemiology and Infection 102, 439-445.
6. Chart, H., Cheasty, T., Cope, D., Gross, R.J. and Rowe, B. (1991b) The serological relationship between Yersinia enterocolitica O9 and Escherichia coli O157 using sera from patients with yersiniosis and haemolytic uraemic syndrome. Epidemiology and Infection 107, 349-356.
7. Chart, H., Cheasty, T., Giorgio, T. and Rowe, B. (1993a) Antigenic cross-reactions between Escherichia coli O157, Vibrio cholerae O1 Inaba and group N Salmonella. Serodiagnosis and Immunotherapy in Infections Diseases 5, 81-84.
8. Chart, H. and Griffiths, K. (1985) Antigenic and molecular homology of the ferric enterobactin receptor protein of Escherichia coli. Journal of General Microbiology 131, 1503-1509.
9. Chart, H., Montgomery, S. and Rowe, B. (1994) A rapid immunoblotting procedure for detecting serum antibodies to the lipopolysaccharide of Escherichia coli O157:H7. Letters in Applied Microbiology 18, 100-101.
10. Chart, H., Okubadejo, O.A. and Rowe, B. (1992) The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis. Epidemiology and Infection 108, 77-85.
11. Chart, H. and Rowe, B. (1997) A simple dot immunoassay for detecting antibodies to the lipopolysaccharide of verocytotoxin-producing Escherichia coli in patients with haemolytic uraemic syndrome. Journal of Microbiology Methods 28, 85-88.
12. Chart, H., Scotland, S.M. and Rowe, B. (1988) Bacterial antigenic cross-reactions and haemolytic uraemic syndrome. Lancet ii, 510-511.
13. Chart, H., Scotland, S.M. and Rowe, B. (1989) Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. Journal of Clinical Microbiology 27, 285-290.
14. Chart, H., Smith, H.R., Scotland, S.M., Rowe, B., Milford, D.V. and Taylor, C.V. (1991a) Serological identification of Escherichia coli O157:H7 infection in haemolytic uraemic syndrome. Lancet 337, 138-140.
15. Chart, H., Willshaw, G.S., Cheasty, T. and Rowe, B. (1993b) Structure and antigenic properties of Citrobacter freundii lipopolysaccharides. Journal of Applied Bacteriology 74, 583-587.
16. Engvall, E. and Perlmann, P. (1972) Enzyme-linked immunosorbent assay, ELISA. III Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen coated tubes. Journal of Immunology 109, 129-135.
17. Greatorex, J.S. and Thorne, G.M. (1994) Humoral immune response to Shiga-like toxin and Escherichia colt 0157 lipopolysaccharide in hemolytic-uremic syndrome patients and healthy subjects. Journal of Clinical Microbiology 32, 1172-1178.
18. Griffin, P.M. and Tauxe, R.V. (1991) The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiology Review 13, 60-98.
19. Johnson, R.P., Cray, W.C. Jr. and Johnson, S.T. (1996) Serum antibody responses of cattle following experimental infection with Escherichia coli O157:H7. Infection and Immunity 64, 1879-1883.
20. Karmali, M.A. (1989) Infection by verocytotoxin-producing Escherichia coli. Clinical Microbiology Reviews 2, 15-38.
21. Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriopbage T4. Nature 227, 680-685.
22. Reymond, D., Johnson, R.P., Karmali, M.A. et al. (1996) Neutralizing antibodies to Escherichia coli verocytotoxin 1 and antibodies to 0157 lipopolysaccharide in healthy farm family members and urban residents. Journal of Clinical Microbiology 34, 2053-2057.
23. Riley, L.W., Remis, R.S., Helgerson, S.D. et al. (1983) Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308, 681-685.
24. Siddons, C.A. and Chapman, P.S. (1993) Detection of serum and faecal antibodies in haemorrhagic colitis caused by Escherichia coli O157. Journal of Medical Microbiology 39, 408-415.
25. Thomas, A., Cheasty, T., Frost, J.A., Chart, H., Smith, H.R. and Rowe, B. (1996) Verocytotoxin producing Escherichia coli, particularly serogroup 0157, associated with human infections in England and Wales: 1992-1994. Epidemiology and Infection 117, 1-10.
26. Thomas, D.R., Salmon, R.L., Kench, S.M. et al. (1994) Zoonotic illness-determining risks and measuring effects: association between current animal exposure and history of illness in a well characterised rural population in the UK. Journal of Epidemiology and Community Health 48, 151-155.
27. Towbin, T., Staehelin, T. and Stark, G.R. (1979) Transfer of proteins from gels to diazobenzyl paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National Academy of Science USA 76, 4350-4354.