A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from cases of bloody diarrhoea, non-bloody diarrhoea and asymptomatic contacts

Journal of Medical Microbiology

Volumn 44, Issue 4, 1996, Pages 267-271

  Chapman, P A ^a   Siddons, C A ^a  

^a Public Health Laboratory   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM ISOLATION; CELL SEPARATION; CLINICAL TRIAL; CONTACT EXAMINATION; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CULTURE MEDIUM; DIAGNOSTIC VALUE; DIARRHEA; ESCHERICHIA COLI; FECES ANALYSIS; HUMAN; MAJOR CLINICAL STUDY; PRIORITY JOURNAL;

ACUTE DISEASE; ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIAL TOXINS; BACTERIOPHAGE TYPING; CEFIXIME; CEFOTAXIME; CERCOPITHECUS AETHIOPS; CULTURE MEDIA; CYTOTOXINS; DIARRHEA; DNA PROBES; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FECES; GASTROINTESTINAL HEMORRHAGE; HUMANS; IMMUNOMAGNETIC SEPARATION; PLASMIDS; RHAMNOSE; SHIGA-LIKE TOXIN I; SORBITOL; VERO CELLS;

EID: 0029930467     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/00222615-44-4-267     Document Type: Article

References (22)

1. Chapman PA, Wright DJ, Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect 1989; 102: 439-145.
2. Chapman PA, Siddons CA, Wright DJ, Norman P, Fox J, Crick E. Cattle as a possible source of verocytotoxin-producing Escherichia coli O157 infections in man. Epidemiol Infect 1993; 111: 439-447.
3. Chapman PA, Wright DJ, Higgins R. Untreated milk as a source of verotoxigenic E. coli O157. Vet Rec 1993; 133: 171-172.
4. Morgan D, Newman CP, Hutchinson DN, Walker AM, Rowe B, Majid F. Verotoxin producing Escherichia coli O157 infections associated with the consumption of yoghurt. Epidemiol Infect 1993; 111: 181-187.
5. Belongia EA, Osterholm MT, Soler JT, Ammend DA, Braun JE, MacDonald KL. Transmission of Escherichia coli O157:H7 infection in Minnesota child day-care facilities. J Am Med Assoc 1993; 269: 883-888.
6. Karmali MA, Arbus GS, Petric M et al. Hospital-acquired Escherichia coli O157:H7 associated haemolytic uraemic syndrome in a nurse. Lancet 1988; 1: 526.
7. Ratnam S, March SB, Sprague WD, Severs D, Sullivan RM. Are humans a source of Escherichia coli O157.H7, the agent of hemorrhagic colitis? N Engl J Med 1986; 315: 1612-1613.
8. March SB, Ratnam S. Sorbitol-MacConkey medium for the detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol 1986; 23: 869-872.
9. Chapman PA, Siddons CA, Zadik PM, Jewes L. An improved selective medium for the isolation of Escherichia coli O157. J Med Microbiol 1991; 35: 107-110.
10. Zadik PM, Chapman PA, Siddons CA. Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157. J Med Microbiol 1993; 39: 155-158.
11. Wright DJ, Chapman PA, Siddons CA. Immunomagnetic separation as a sensitive method for isolating Escherichia coli O157 from food samples. Epidemiol Infect 1994; 113: 31-39.
12. Chapman PA, Wright DJ, Siddons CA. A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from bovine faeces. J Med Microbiol 1994; 40: 424-427.
13. Board RG. A modern introduction to food microbiology. (Basic microbiology vol 8). Oxford, Blackwell Scientific Publications. 1983.
14. Chapman PA, Daly CM. Evaluation of non-radioactive trivalent DNA probe (LTh, ST1a, ST1b) for detecting enterotoxigenic Escherichia coli. J Clin Pathol 1993; 46: 309-312.
15. Chapman PA, Jewes L, Siddons CA, Norman P, George SL. Verocytotoxin-producing Escherichia coli infections in Sheffield: 1985-1989. PHLS Microbiol Digest 1990; 7: 163-166.
16. Borczyk AA, Karmali MA, Lior H, Duncan LMC. Bovine resevoir for verotoxin-producing Escherichia coli O157:H7. Lancet 1987; 1: 98.
17. Martin ML, Shipman LD, Wells JG et al. Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of haemolytic-uraemic syndrome. Lancet 1986; 2: 1043.
18. Ostroff SM, Griffin PM, Tauxe RV et al. A statewide outbreak of Escherichia coli O157:H7 infections in Washington state. Am J Epidemiol 1990; 132: 239-247.
19. Wells JG, Shipman LD, Greene KD et al. Isolation of Escherichia coli serotype O157:H7 and other shiga-like toxin-producing E. coli from dairy cattle. J. Clin Microbiol 1991; 29: 985-989.
20. Mechie SC, Chapman PA, Siddons CA. A fifteen month field study of Escherichia coli O157:H7 in a dairy herd. (in press)
21. Tarr PI, Neill MA, Clausen CR, Watkins SL, Christie DL, Hickman RO. Escherichia coli O157:H7 and the hemolytic uremic syndrome: importance of early cultures in establishing the etiology. J Infect Dis 1990; 162: 553-556.
22. Gransden WR, Damm MAS, Anderson JD, Carter JE, Lior H. Haemorrhagic cystitis and balanitis associated with verotoxin-producing Escherichia coli O157:H7. Lancet 1985; 2: 150.