A simple dot immunoassay for detecting antibodies to the lipopolysaccharide of Verocytotoxin-producing Escherichia coli in patients with haemolytic uraemic syndrome

Journal of Microbiological Methods

Volumn 28, Issue 1, 1997, Pages 85-88

  Chart, Henrik ^a   Rowe, Bernard ^a  

^a CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

Author keywords

dot blot; Escherichia coli; human antibodies; immunoassay; serology; Verocytotoxin

Indexed keywords

LIPOPOLYSACCHARIDE; VEROTOXIN;

ANTIBODY DETECTION; ARTICLE; BLOOD GROUP TYPING; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; DOT HYBRIDIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HEMOLYTIC UREMIC SYNDROME; HUMAN; IMMUNOASSAY; PRIORITY JOURNAL;

EID: 0031047237     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(96)00968-2     Document Type: Article

References (11)

1. Karmali, M.A. (1989) Infection by Verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2, 15-38.
2. Kleanthos, H., Smith, H.R., Scotland, S.M., Gross, R.J., Rowe, B., Taylor, C.M. and Milford, D.V. (1990) Haemolytic uraemic syndrome in the British Isles, 1985-1988: association with Vero cytotoxin-producing Escherichia coli Part 2, Microbiological aspects. Arch. Dis. Child. 65, 722-727.
3. Smith, H.R. and Scotland, S.M. (1988) Verocytotoxin-producing strains of Escherichia coli. J. Med. Microbiol. 26, 77-85.
4. Chart, H., Scotland, S.M. and Rowe, B. (1989) Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J. Clin. Microbiol. 27, 285-290.
5. Chart, H., Scotland, S.M., Smith, H.R. and Rowe, B. (1989) Antibodies to Escherichia coli in patients with haemorrhagic colitis and haemolytic uraemic syndrome. J. Clin. Pathol. 42, 973-976.
6. Chart, H., Smith, H.R., Scotland, S.M., Rowe, B., Milford, D.V. and Taylor, C.V. (1991) Serological identification of Escherichia coli O157:H7 infection in haemolytic uraemic syndrome. Lancet 337, 138-140.
7. Thomas, A., Chart, H., Cheasty, T., Smith, H.R., Frost, J.A. and Rowe, B. (1993) Verocytotoxin-producing Escherichia coli, particularly serogroup O157, associated with human infections in the United Kingdom: 1989-91. Epidemiol. Infect. 110, 591-600.
8. Chart, H. and Rowe, B. (1990) Serological identification of infection by Verocytotoxin-producing Escherichia coli in patients with haemolytic uraemic syndrome. Serodiagn. Immunother. Infect. Dis. 4, 413-418.
9. Hitchcock, P.J. and Brown, T.M. (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154, 269-277.
10. Laemlli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
11. Chart, H., Smith, H.R. and Rowe, B. (1994) Antigenic cross-reactions between fimbriae expressed by Salmonella enteritidis, S. dublin and an 18 kDa outer membrane associated protein expressed by Escherichia coli O126:H27. FEMS Microbiol. Lett. 121, 19-24.