Phosphorylation of the pheromone-responsive G(β) protein of Saccharomyces cerevisiae does not affect its mating-specific signaling function

Molecular and General Genetics

Volumn 258, Issue 6, 1998, Pages 608-618

  Li, E ^a,b   Cismowski, M J ^a   Stone, D E ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b Cadus Pharmaceutical Corporation   (United States)

Author keywords

Adaptation; G( ) phosphorylation; Mating response; Saccharomyces cerevisiae; Signal transduction

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PHEROMONE; PROTEIN SUBUNIT;

ADAPTIVE BEHAVIOR; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DELETION MUTANT; GENE DISRUPTION; GENE MUTATION; MATING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; YEAST CELL;

AMINO ACID SEQUENCE; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; FUNGAL PROTEINS; GTP-BINDING PROTEIN ALPHA SUBUNITS; GTP-BINDING PROTEIN BETA SUBUNITS; GTP-BINDING PROTEINS; HETEROTRIMERIC GTP-BINDING PROTEINS; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; PHEROMONES; PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE; SIGNAL TRANSDUCTION; THREONINE;

SACCHAROMYCES CEREVISIAE;

EID: 0031850002     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050774     Document Type: Article

References (46)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1994) Current protocols in molecular biology. John Wiley and Sons, New York
2. Chien C-T, Bartel PL, Sternglanz R, Fields S (1991) The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad USA 88:9578-9582
3. Choi K-Y, Satterberg B, Lyons D, Elion EA (1994) Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell 78:499-512
4. Cole GM, Reed SI (1991) Pheromone-induced phosphorylation of a G protein ß subunit in S. cerevisiae is associated with an adaptive response to mating pheromone. Cell 64:703-716
5. Cole GM, Stone DE, Reed SI (1990) Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway. Mol Cell Biol 10:510-517
6. Dietzel C, Kurjan J (1987) Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone. Mol Cell Biol 7:4169-4177
7. Dohlman HG, Apanisek D, Chen Y, Song J, Nusskern D (1995) Inhibition of G-protein signaling by mutants with dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Sacharomyces cerevisiae. Mol Cell Biol 15:3635-3643
8. Dohlman HG, Song J, Ma D, Courchesne WE, Thorner J (1996) Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, genetic interaction and physical association with Gpa1 (G protein α subunit). Mol Cell Biol 16:5194-5209
9. Doi K, Gartner A, Ammerer G, Errede B, Shinkawa H, Sugimoto K, Matsumoto K (1994) MSG5, a novel protein phosphatase promotes adaptation to pheromone response in 5. cerevisiae. EMBO J 13:61-70
10. Elion EA, Brill JA, Fink GR (1991) FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc Natl Acad Sci USA 88:9392-9396
11. β and the LIM domain of Ste5 is required to activate the MEKK Stel1. Curr Biol 8:267-278
12. Fields S, Song O-K (1989) A novel genetic system to detect protein-protein interactions. Nature 340:245-246
13. Gartner A, Nasmyth K, Ammerer G (1992) Signal transduction is Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1. Genes Dev 6:1280-1292
14. Gietz RD, Sugino A (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534
15. Guthrie C, Fink GR (eds) (1991) Guide to yeast genetics and molecular biology. Academic Press, San Diego
16. Hall JP, Cherkasova V, Elion E, Gustin MC, winter E (1996) The osmoregulatory pathway represses mating pathway activity in Saccharomyces cerevisiae: isolation of a FUS3 mutant that is insensitive to the repression mechanism. Mol Cell Biol 16:6715-6723
17. Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
18. Inglese J, Koch WJ, Touhara K, Lefkowitz RJ (1995) Gβγ interactions with PH domains and Ras-MAPK signaling pathways. Trends Biochem Sci 20:151-156
19. Irie K, Nomoto S, Miyajima I, Matsumoto K (1991) SGV1 encodes a CDC28/cdc2-related kinase required for a Gα subunit-mediated adaptive response to pheromone in S. cerevisiae. Cell 65:785-795
20. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163-168
21. Konopka JB, Jenness DD, Hartwell LH (1988) The C-terminus of the Saccharomyces cerevisiae α-pheromone receptor mediates an adaptive response to pheromone. Cell 54:609-620
22. Kranz JE, Satterberg B, Elion EA (1994) The MAP kinase FUS3 associates with and phosphorylates the upstream signaling component. Genes Dev 8:313-327
23. Kurjan J (1992) Pheromone response in yeast. Annu Rev Biochem 61:1097-1129
24. Leberer E, Dignard D, Hougan L, Thomas DY, Whiteway M (1992) Dominant-negative mutants of a yeast G-protein β subunit identify two functional regions involved in pheromone signalling. EMBO J 11:4805-4813
25. Leeuw T, Wu C, Schrag JD, Whiteway M, Thomas DY, Leberer E (1998) Interaction of a G-protein β-subunit with a conserved sequence in Ste20/PAK family protein kinases. Nature 391:191-195
26. β protein of Saccharomyces cerevisiae confer a defect in recovery from pheromone treatment. Genetics 148:947-961
27. MacKay VL, Welch SK, Insley MY, Manney TR, Holley J, Saaru GC, Parker ML (1988) The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin. Proc Natl Acad Sci USA 85:55-59
28. Madhani HD, Styles CA, Fink GR (1997) MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell 91:673-684
29. 1 arrest. Mol Cell Biol 11:1030-1039
30. Marcus S, Polverino A, Barr M, Wigler M (1994) Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc Natl Acad Sci USA 91:7762-7766
31. McCaffrey G, Clay FJ, Kelsay K, Sprague GF Jr (1987) Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol Cell Biol 7:2680-2690
32. Mukhopadhyay NK, Price DJ, Kyriakis JM, Pelech S, Sanghera J, Avruch J (1992) An array of insulin-activated, proline directed serine threonine protein kinases phosphorylate the p70 S6 kinase. J Biol Chem 267:3325-3335
33. Printen JA, Sprague GF Jr (1994) Protein-protein interactions in the yeast pheromone response pathway: Sle5p interacts with all members of the MAP kinase cascade. Genetics 138:609-619
34. Reed SI, Hadwiger JA, Lorincz AT (1985) Protein kinase activity associted with the product of the yeast cell division cycle gene CDC28. Proc Natl Acad Sci USA 82:4055-4059
35. Reneke JE, Blumer KJ, Courchesne WE, Thorner J (1988) The carboxy-terminal segment of the yeast α-factor receptor is a regulatory domain. Cell 55:221-234
36. Roberts RL, Fink GR (1994) Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev 8:2974-2985
37. Rothstein R (1991) Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol 194:281-301
38. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
39. Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
40. Slater MR, Craig EA (1987) Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae. Mol Cell Biol 7:1906-1916
41. Sprague GF, Thorner JW (1992) Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae. In: Jones E-W, Pringle JR, Broach JR (eds) The Molecular and cellular biology of the yeast Saccharomyces cerevisiae. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
42. Stoffel RH, Pitcher JA, Lefkowitz RJ (1997) Targeting G protein-coupled receptor kinases to their receptor substrates. J Membrane Biol 157:1-8
43. βγ sequestration. Mol Cell Biol 16:6325-6337
44. Trueheart J, Boeke JD, Fink GR (1987) Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol Cell Biol 7:2316-2328
45. Whiteway MS, Wu C, Leeuw T, Clark K, Fourest-Lieuvin A, Thomas DY, Leberer E (1995) Association of the yeast pheromone response G protein βγ subunits with the MAP kinase scaffold Ste5p. Science 269:1572-1574
46. Zhou Z, Gartner A, Cade R, Ammerer G, Errede B (1993) Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases. Mol Cell Biol 13:2069-2080