Substitutions in the pheromone-responsive G(β) protein of Saccharomyces cerevisiae confer a defect in recovery from pheromone treatment

Genetics

Volumn 148, Issue 3, 1998, Pages 947-961

  Li, E ^a   Meldrum, Eric ^b,c   Stratton, Holly F ^a   Stone, David E ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c GLAXO INST FOR MOLECULAR BIOLOGY   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; MUTANT PROTEIN; PHEROMONE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; GENE LOCUS; GENE MUTATION; GENE SEQUENCE; HORMONE ACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE;

ADAPTATION, PHYSIOLOGICAL; FUNGAL PROTEINS; GTP-BINDING PROTEIN ALPHA SUBUNITS; GTP-BINDING PROTEIN BETA SUBUNITS; GTP-BINDING PROTEINS; HETEROTRIMERIC GTP-BINDING PROTEINS; MUTAGENESIS; PEPTIDES; PHEROMONES; PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0031972043     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. AUSUBEL, F. M., R. BRENT, R. E. KINGSTON, D. D. MDORE, J. G. SEIDMAN et al., 1994 Current Protocols in Molecular Biology. John Wiley & Sons, New York.
2. BLINDER, D., S. BOUVIER and D. D. JENNESS, 1989 Constitutive mutants in the yeast pheromone response: ordered function of the gene products. Cell 56: 479-486.
3. CLAPHAM, D. E., and E. J. NEER, 1993 New roles for G-protein βγ-dimers in transmembrane signalling. Nature 365: 403-406.
4. COLE, G. M., and S. I. REED, 1991 Pheromone-induced phosphorylation of a G protein β subunit in S. cerevisiae is associated with an adaptive response to mating pheromone. Cell 64: 703-716.
5. COLE, G. M., D. E. STONE and S. I. REED, 1990 Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway. Mol. Cell. Biol. 10: 510-517.
6. αs-cAMP-mediated inhibition. J. Biol. Chem. 270: 25259-25265.
7. CROSS, F., 1988 DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol. Cell Biol. 8: 4675-4684.
8. CROSS, F., L. H. HARTWELL, C. JACKSON and J. B. KONOPKA, 1988 Conjugation in Saccharomyces cerevisiae. Annu. Rev. Cell. Biol. 4: 429-457.
9. DOI, K., A. GARTNER, G. AMMERER, B. ERREDE, H. SHINKAWA et al., 1994 MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J. 13: 61-70.
10. GAUDET, R., A. BOHM and P. SIGLER, 1996 Crystal structure at 2.4 Å resolution of the complex of transducin βγ and its regulator, phosducin. Cell 87: 577-588.
11. GIETZ, R. D., and A. SUGINO, 1988 New yeast - Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
12. GRISHIN, A. V., J. L. WEINER and K. J. BLUMER, 1994 Control of adaptation to mating pheromone by G protein β subunits of Saccharomyces cerevisiae. Genetics 138: 1081-1092.
13. HARLOW, E., and D. LANE, 1988 Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor Press, NY.
14. INIGUEZ-LLUHI, J., C. KLEUSS and A. G. GILMAN, 1993 The importance of G-protein βγ subunits. Trends Cell Biol. 3: 230-236.
15. ITO, H., Y, FUKUDA, K. MURATA and A. KIMURA, 1983 Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163-168.
16. KAO, L.-R., J. PETERSON, R. JI, L. BENDER and A. BENDER, 1996 Interactions between the ankyrin repreat-containing protein akrlp and the pheromone response pathway in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 168-178.
17. KONOPKA, J. B., D. D. JENNESS and L. H. HARTWELL, 1988 The C-terminus of the Saccharomyces cerevisiae α-pheromone receptor mediates an adaptive response to pheromone. Cell 54: 609-620.
18. KURJAN, J., 1992 Pheromone response in yeast. Annu. Rev. Biochem. 61: 1097-1129.
19. LAMBRIGHT, D. G., J. SONDEK, A. BOHM, N. P. SKIBA, H. E. HAMM et al., 1996 The 2.0 Å crystal structure of a heterotrimeric G protein. Nature 379: 311-319.
20. LEE, E., R. TAUSSIG and A. G. GILMAN, 1992 The G226A mutant of Gsα highlights the requirement for dissociation of G protein subunits. J. Biol. Chem. 267: 1212-1218.
21. LIU, M., and M. I. SIMON, 1996 Regulation by cAMP-dependent protein kinease of a G-protein-mediated phopholipase C. Nature 382: 83-87.
22. MACKAY, V. L., S. K. WELCH, M. Y. INSLEY, T. R. MANNEY, J. HOLLEY et al., 1988 The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin. Proc. Natl. Acad. Sci. USA 85: 55-59.
23. 1 arrest. Mol. Cell. Biol. 11: 1030-1039.
24. ORR-WEAVER, T. L., J. W. SZOSTAK and R. J. ROTHSTEIN, 1983 Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol. 101: 228-245.
25. βγ complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction. Mol. Cell. Biol. 16: 2614-2626.
26. REED, S. I., J. A. HADWIGER and A. T. LORINCZ, 1985 Protein kinase activity associted with the product of the yeast cell division cycle gene CDC28. Proc. Natl. Acad. Sci. USA 82: 4055-4059.
27. RENEKE, J. E., K. J. BLUMER, W. E. COURCHESNE and J. THORNER, 1988 The carboxy-terminal segment of the yeast α-factor receptor is a regulatory domain. Cell 55: 221-234.
28. ROTHSTEIN, R., 1991 Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 194: 281-301.
29. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. + channel by G-protein α-subunits. Nature 380: 624-627.
31. SCHWARTZ, R. M., and M. O. DAYHOFF, 1979 Atlas of protein sequence and structure. National Biomedical Research Foundation, Washington, DC.
32. SHERMAN, F., G. R. FINK and J. B. HICKS, 1986 Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
33. SIMON, M.-N., C. D. VIRGILIO, B. SOUZA, J. R. PRINGLE, A. ABO et al., 1995 Role for the Rho-family GTPase Cdc42 in yeast matingpheromone signal pathway. Nature 376: 702-705.
34. SLATER, M. R., and E. A. CRAIG, 1987 Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae. Mol. Cell Biol. 7: 1906-1916.
35. α protein βγ dimer at 2.1 Å resolution. Nature 379: 369-374.
36. SPAIN, B. H., D. KOO, M. RAMAKRISHNAN, B. DZUDZOR and J. COLICELLI, 1995 Truncated forms of a novel yeast protein suppress the lethality of a G protein a subunit deficiency by interacting with the β subunit. J. Biol. Chem. 270: 25435-25444.
37. SPRAGUE, G. F., and J. W. THORNER, 1992 Pheromone Response and Signal Transduction during the Mating Process of Saccharomyces cerevisiae. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
38. STONE, D. E., and S. I. REED, 1990 G protein mutations that alter the pheromone response in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 4439-4446.
39. α protein of yeast: Conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase. Genes Dev. 5: 1969-1981.
40. βγ sequestration. Mol. Cell. Biol. 16: 6325-6337.
41. TATCHELL, K., K. A. NASMYTH and B. D. HALL, 1981 In vitro mutation analysis of the niating-type locus in yeast. Cell 27: 25-35.
42. TRUEHEART, J., J. D. BOEKE and G. R. FINK, 1987 Two genes required for cell fusion during yeast conjugation: Evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 7: 2316-2328.
43. WHITEWAY, M., I. HOUGAN, D. DIGNARD, D. Y. THOMAS, L. BELL et al., 1989 The STE4 and STE18 genes encode potential β and γ subnnits of the mating factor coupled G protein. Cell 56: 467-477.
44. WHITEWAY, M., K. L. CIARK, E. LEBERER, D. DIGNARD and D. Y. THOMAS, 1994 Genetic identification of residues involved in association of α and β G-protein subunits. Mol. Cell. Biol. 14: 3223-3229.
45. WHITEWAY, M. S., C. WU, T. LEEUW, K. CLARK, A. FOUREST-LIEUVIN et al., 1995 Association of the yeast pheromone response G protein βγ subunits with the MAP kinase scaffold ste5p. Science 269: 1572-1574.
46. ZHAO, Z., T. LEUNG, E. MANSER and L. LIM, 1995 Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24. Mol. Cell. Biol. 15: 5246-5257.