Glutamate-Induced Efflux of Protein, Neuron-Specific Enolase and Lactate Dehydrogenase From a Mesencephalic Cell Culture1)

Clinical Chemistry and Laboratory Medicine

Volumn 34, Issue 4, 1996, Pages 305-310

  Gross, Johann ^a   Ungethüm, Ute ^a   Heldt, Julia ^a   Priem, Friedrich ^a   Marschhausen, Grit ^a   Altmann, Tliomas ^a   Andreeva, Nadejda ^b  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b BRAIN RESEARCH INSTITUTE   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; GLUTAMIC ACID; LACTATE DEHYDROGENASE; NEURON SPECIFIC ENOLASE; SODIUM CHLORIDE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; ENZYME ACTIVITY; FEMALE; FETUS; IMMUNOCOMPETENT CELL; MESENCEPHALON; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; RAT;

ANIMALS; CELLS, CULTURED; FEMALE; GLUTAMIC ACID; L-LACTATE DEHYDROGENASE; MESENCEPHALON; NEURONS; PHOSPHOPYRUVATE HYDRATASE; PREGNANCY; RATS; RATS, WISTAR;

ANIMALIA;

EID: 0030002962     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/cclm.1996.34.4.305     Document Type: Article

References (19)

1. Marangos PJ, Zomzely-Neurath C, Goodwin FK. Structural and functional properties of neuron specific protein (NSP) from rat, cat and human brain. J Neurochem 1977; 28: 1097-1107.
2. Marangos PJ. Neuron specific enolase as a clinical tool. In: Grätzel M, Langley K, editors. Neurologic and endocrine disease markers for neural and endocrine cells. New York, Basel, Cambridge, Weinheim: 1990: 181-189.
3. Hans P, Bonhomme V, Collette J, Moonen G. Neuron-specific enolase as a marker of in vitro neuronal damage Part I: Assessment of neuron-specific enolase as a quantitative and specific marker of neuronal damage. J Neurosurg Anesthesiol 1993; 5: 111-116.
4. Marangos PJ, Goodwin FK. Neuron specific protein (NSP) in neuroblastoma cells: relation to differentiation. Brain Res 1978; 145: 49-58.
5. Schilling K, Barco EB, Rhinehart D, Pilgrim C. Expression of synaptophysin and neuron-specific enolase during neuronal differentiation in vitro: effects of dimethyl sulfoxide. J Neurosci Res 1989; 24: 247-254.
6. Cook JA, Mitchell JB. Viability measurements in mammalian cell systems. Anal Biochem 1989; 179: 1-7.
7. Koh JY, Choi DW. Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. J Neurosci Methods 1987; 20: 83-90.
8. Andreeva N, Ungethüm U, Heldt J, Gross J. Elevated potassium enhances glutamate vulnerability of dopaminergic neurons developing in mesencephalic cell culture. Exp Neurology. 1996; 137: 255-262.
9. Goldberg MP, Weiss JH, Pham PC, Choi DW. N-Methyl-Daspartate receptors mediate hypoxic neuronal injury in cortical culture. J Pharm Exp Ther 1987; 243: 784-791.
10. Choi DW. Ionic dependence of glutamate neurotoxicity. J Neurosci 1987; 7: 469-479.
11. Comer AJ, Lees GJ, Lipski J. Neurone-specific enolase persits in some hippocampal cells 24 hours after NMDA injection. Neuroreport 1994; 5: 2589-2592.
12. Lowry DH, Rosebrough NJ, Fair AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
13. Priestley T, Home AL, McKernan RM, Kemp JA. The effect of NMDA receptor glycine site antagonists on hypoxia-induced neurodegeneration of rat cortical cell cultures. Brain Res 1990; 531: 183-188.
14. Schmidt E, Schmidt FW. Enzyme release. J Clin Chem Clin Biochem 1987; 25: 525-540.
15. Gross J, Ungethüm U, Moller R, Priem F, Heldt J, Ziebig R, Pawlow I. Präanalytische Faktoren der NSE-Bestimmung im Blut. Lab Med 1995; 19: 286-289.
16. Lim L, Hall C, Leung T, Mahadevan L, Whatley S. Neuronespecific enolase and creatine phosphokinase are protein components of rat brain synaptic plasma membranes. J Neurochem 1983; 41: 1177-1182.
17. Diederichs F, Muhlhaus K, Wittenberg H, Trautschold I. Enzyme release from the perfused rat heart. The functions of the cytoskeleton under cell-pathological conditions. J Clin Chem Clin Biochem 1986; 24: 3-9.
18. Lipton SA, Choi YB, Pan ZH, Lei SZ, Chen HSV, Sucher NJ, et al. A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitrosocompounds. Nature 1993; 364: 626-632.
19. Juurlink BHJ, Hertz L. Ischemia-induced death of astrocytes and neurons in primary culture: pitfalls in quantifying neuronal cell death. Dev Brain Res 1993; 71: 239-246.