Nuclear import of protein kinase C occurs by a mechanism distinct from the mechanism used by proteins with a classical nuclear localization signal

Journal of Cell Science

Volumn 111, Issue 13, 1998, Pages 1823-1830

  Schmalz, Dirk ^a   Hucho, Ferdinand ^a   Buchner, Klaus ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Cell nucleus; Nuclear transport; Phorbol eater; Protein kinase C

Indexed keywords

KARYOPHERIN; PHORBOL ESTER; PROTEIN KINASE C;

ARTICLE; CELL NUCLEUS; CELL STRAIN 3T3; INTRACELLULAR TRANSPORT; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT;

3T3 CELLS; ALPHA KARYOPHERINS; ANIMALS; BETA KARYOPHERINS; BIOLOGICAL TRANSPORT, ACTIVE; CELL NUCLEUS; CYTOSKELETON; DIFFUSION; ISOENZYMES; MICE; NUCLEAR LOCALIZATION SIGNALS; NUCLEAR PROTEINS; PROTEIN KINASE C; PROTEIN KINASE C-ALPHA; RAN GTP-BINDING PROTEIN;

BOVINAE; TRITICUM AESTIVUM;

EID: 0031826010     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)

1. Adam, S. A., Sterne-Marr, R. and Gerace, L. (1990). Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell Biol. 111, 807-816.
2. Beckmann, R., Lindschau, C., Haller, H., Hucho, F. and Buchner, K. (1994). Differential nuclear localization of protein kinase C isoforms in neuroblastoma × glioma hybrid cells. Eur. J. Biochem. 222, 335-343.
3. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
4. Breeuwer, M. and Goldfarb, D. S. (1990). Facilitated nuclear transport of histone H1 and other small nucleophilic proteins. Cell 60, 999-1008.
5. Buchner, K. (1995). Protein kinase C in the transduction of signals toward and within the cell nucleus. Eur. J. Biochem. 228, 211-221.
6. Chi, N., Adam, E. and Adam, S. (1995). Sequence and characterization of cytoplasmic nuclear protein import factor p97. J. Cell Biol 130, 265-274.
7. Davis, L. I. and Blobel, G. (1986). Identification and characterization of a nuclear pore complex protein. Cell 45, 699-709.
8. Dekker, L. V. and Parker, P. J. (1994). Protein kinase C - a question of specificity. Trends Biochem. Sci. 19, 73-77.
9. Dingwall, C. and Laskey, R. A. (1991). Nuclear targeting sequences - a consensus? Trends Biochem. Sci. 16, 478-481.
10. Disatnik, M.-H., Buraggi, G. and Mochly-Rosen, D. (1994). Localization of protein kinase C isozymes in cardiac myocytes. Exp. Cell Res. 210, 287-297.
11. Goodnight, J., Mischak, H. and Mushinski, J. F. (1994). Selective involvement of protein kinase C isozymes in differentiation and neoplastic transformation. Advan. Cancer Res 64, 159-209.
12. Görlich, D., Prehn, S., Laskey, R. A. and Hartmann, E. (1991). Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79, 767-778.
13. Görlich, D., Vogel, F., Mills, A., Hartmann, E. and Laskey, R. (1995). Distinct functions for the two importin subunits in nuclear protein import. Nature 377, 246-248.
14. Görlich, D. and Mattaj, I. (1996). Nucleocytoplasmic transport. Science 271, 1513-1518.
15. Görlich, D., Pante, N., Kutay, U., Aebi, U. and Aebi, U., FR (1996). Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15, 5584-5594.
16. Hamm-Alvarez, S., Alayof, B., Himmel, H., Kim, P., Strauss, H., Crews, A. and Sheetz, M. (1994). Coordinate depression of bradykinin receptor recycling and microtubule-dependent transport of taxol. Proc. Nat. Acad. Sci. USA 91, 7812-7816.
17. Hyatt, S. L., Liao, L., Chapline, C. and Jaken, S. (1994). Identification and characterization of alpha-protein kinase C binding proteins in normal and transformed REF52 cells. Biochemistry 33, 1223-1228.
18. Jaken, S. (1992). PKC interactions with intracellular components. In Protein Kinase C. Current Concepts and Future Perspectives (ed. D. S. Lester and R. M. Epand), pp. 237-254. Chichester: Ellis Horwood Limited.
19. Leach, K. L., Powers, E. A., Ruff, V. A., Jaken, S. and Kaufmann, S. (1989). Type 3 protein kinase C localization to the nuclear envelope of phorbol ester-treated NIH 3T3 cells. J. Cell Biol. 109, 685-695.
20. Marshallsay, C. and Lührmann, R. (1994). In vitro nuclear import of snRNPs: cytosolic factors mediate m3G-cap dependence of U1 and U2 snRNP transport. EMBO J. 13, 222-231.
21. Marshallsay, C., Dickmanns, A., Bischoff, F., Ponstingl, H., Fanning, E. and Lührmann, R. (1996). In vitro and in vivo evidence that protein and U1 snRNP nuclear import in somatic cells differ in their requirement for GTP-hydrolysis, Ran/TC4 and RCC1. Nucl. Acids Res. 24, 1829-1836.
22. Melchior, F., Paschal, B., Evans, J. and Gerace, L. (1993). Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123, 1649-1659.
23. Michael, M., Eder, P. and Dreyfuss, G. (1997). The K nuclear shuttling domain: a novel signal for nuclear import and nuclear export in the hn RNP K protein. EMBO J. 16, 3587-3598.
24. Michaud, N. and Goldfarb, D. (1992). Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguihable targeting pathways. J. Cell Biol. 116, 851-861.
25. Mochly-Rosen, D., Khaner, H. and Lopez, J. (1991). Identification of intracellular receptor proteins for activated protein kinase C. Proc. Nat. Acad. Sci. USA 88, 3997-4000.
26. Moore, M. S. and Blobel, G. (1992). The two steps of nuclear import, targeting to the nuclear envelope and translocation through the pore, require different cytosolic factors. Cell 69, 939-950.
27. Moore, M. S. and Blobel, G. (1993). The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature 365, 661-663.
28. Moore, M. S. and Blobel, G. (1994). Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Nat. Acad. Sci. USA 91, 10212-10216.
29. Moroianu, J., Blobel, G. and Radu, A. (1995). Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes. Proc. Nat. Acad. Sci. USA 92, 2008-1011.
30. Newton, A. C. (1995). Protein kinase C: Structure, function and regulation. J. Biol. Chem. 270, 28495-28498.
31. Nigg, E. (1997). Nucleoplasmic transport: signals, mechanisms and regulation. Nature 386, 779-787.
32. Nishizuka, Y. (1995). Protein kinase C and lipid signaling for sustained cellular responses. FASEB J. 9, 484-496.
33. Palacios, I., Weis, K., Klebe, C., Mattaj, I. W. and Dingwall, C. (1996). Ran/TC4 mutants identify a common requirement for sn RNP and protein import into the nucleus. J. Cell Biol. 133, 485-494
34. Paschal, B. M. and Gerace, L. (1995). Identification of NTF-2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell Biol. 129, 925-937
35. Pollard, V. W., Michael, W. M., Nakielny, S., Siomi, M. C., Wang, F. and Dreyfuss, G. (1996). A novel receptor-mediated nuclear protein import pathway. Cell 86, 985-994.
36. Radu, A., Blobel, G. and Moore, M. (1995). Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc. Nat. Acad. Sci. USA 92, 1769-1773.
37. Rexach, M. and Blobel, G. (1995). Protein import into nuclei: association and dissociation reactions involving transport, substrate, transport factors and nucleoporins. Cell 83, 683-692.
38. Richards, S., Carey, K. and Macara, I. (1997). Requirement of guanosine triphosphate-bound Ran for signal-mediated nuclear protein export. Science 276, 1842-1844.
39. Ron, D., Chen, C. H., Caldwell, J., Jamieson, L., Orr, E. and Mochly-Rosen, D. (1994). Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins. Proc. Nat. Acad Sci. USA 91, 839-843.
40. Schiff, P. and Horwitz, S. (1980). Taxol stabilizes microtubules in mouse fibroblast cells. Proc. Nat. Acad. Sci. USA 77, 1561-1565.
41. Schmalz, D., Kalkbrenner, F., Hucho, F. and Buchner, K. (1996). Transport of protein kinase C alpha into the nucleus requires intact cytoskeleton while the transport of a protein containing a canonical nuclear localization signal does not. J. Cell Sci. 109, 2401-2406.
42. Staudinger, J., Zhou, J., Burgess, R., Elledge, S. J. and Olson, E. N. (1995). PICK 1: A perinuclear binding protein and substrate for protein kinase C isolated by the yeast two hybrid system. J Cell Biol. 128, 263-271.
43. Sweitzer, T. and Hanover, J. (1996). Calmodulin activates nuclear protein import: A link between signal transuction and nuclear transport. Proc. Nat. Acad. Sci. USA 93, 14574-14579
44. Weis, K., Ryder, U. and Lamond, A. (1996). The conserved amino-terminal domain of hRSP 1 alpha is essential for nuclear protein import. EMBO J. 15, 1818-1825
45. Yaseen, N. and Blobel, G. (1997). Cloning and characterization of human karyopherin-beta 3. Proc. Nat. Acad. Sci. USA 94, 4451-4456.