Use of green fluorescent protein to tag and investigate gene expression in marine bacteria

Applied and Environmental Microbiology

Volumn 64, Issue 7, 1998, Pages 2554-2559

  Stretton, Serina ^a   Techkarnjanaruk, Somkiet ^a   Mclennan, Alan M ^a   Goodman, Amanda E ^a  

^a FLINDERS UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CHITIN; GREEN FLUORESCENT PROTEIN;

BACTERIUM; FLUORESCENCE; FLUOROSCENCE; MARINE ENVIRONMENT; TAGGING;

ARTICLE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; MARINE ENVIRONMENT; NONHUMAN; SOIL MICROFLORA; STRAIN DIFFERENCE; TRANSPOSON; VIBRIO;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS SP.; PSYCHOBACTER; PSYCHROBACTER; PSYCHROBACTER SP.; VIBRIO; VIBRIO SP.;

EID: 0031816320     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.7.2554-2559.1998     Document Type: Article

References (42)

1. Albertson, N. H., T. Nystrom, and S. Kjelleberg. 1990. Exoprotease activity of two marine bacteria during starvation. Appl. Environ. Microhiol. 56:218-223.
2. Albertson, N. H., S. Stretton, S. Pongpattanakitshote, S. Kjelleberg, and A. E. Goodman. 1996. Construction and use of a new mini-Tn10:lac:kan transposon to identify environmentally responsive genes. FEMS Microbiol. Lett. 140:287-294.
3. Angles, M. L., K. C. Marshall, and A. E. Goodman. 1993. Plasmid transfer between marine bacteria in the aqueous phase and biofilms in reactor microcosms. Appl. Environ. Microbiol. 59:843-850.
4. Baumberg, S., G. Cornelis, M. Panagiotakopoulos, and M. Roberts. 1980. Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas. J. Gen. Microbiol. 119:257-262.
5. Bloemberg, G. V., G. A. O'Toole, B. J. J. Lugtenberg, and R. Kolter. 1997. Green fluorescent protein as a marker for Pseudomonas spp. Appl. Environ. Microbiol. 63:4543-4551.
6. Burlage, R. S., Z. K. Yang, and T. Mehlhorn. 1996. A transposon for green fluorescent protein transcriptional fusions: application for bacterial transport experiments. Gene 173:53-58.
7. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
8. Chen, F., M. J. González, A. W. Dustman, A. M. Moran, and R. E. Hodson. 1997. In situ reverse transcription, an approach to characterize genetic diversity and activities of prokaryotes. Appl. Environ. Microbiol. 63:4997-4913.
9. Christensen, B. B., C. Sternberg, and S. Molin. 1996. Bacterial plasmid conjugation on semi-solid surfaces monitored with the green fluorescent protein (GFP) from Aequorea victoria as a marker. Gene 173:59-65.
10. Cormack, B. P., R. H. Valdivia, and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
11. Dalton, H. M., A. E. Goodman, and K. C. Marshall. 1996. Diversity in surface colonization behaviour in marine bacteria. J. Ind. Microbiol. 17:228-234.
12. Davies, D. G., and G. G. Geesey. 1995. Regulation of the alginate biosynthesis gene algC in Pseudomonas aeruginosa during biofilm development in continuous culture. Appl. Environ. Microbiol. 61:860-867.
13. Dhandayuthapani, S., L. E. Via, C. A. Thomas, P. M. Horowitz, D. Deretic, and V. Deretic. 1995. Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages. Mol. Microbiol. 17:901-912.
14. Eberl, L., R. Schulze, A. Ammendola, O. Geisenberger, R. Erhart, C. Sternberg, S. Molin, and R. Amann. 1997. Use of green fluorescent protein as a marker for ecological studies of activated sludge communities. FEMS Microbiol. Lett. 149:77-83.
15. Gage, D. J., T. Bobo, and S. R. Long. 1996. Use of green fluorescent protein to visualize the early events of symbiosis between Rhizobium meliloti and alfalfa (Medicago sativa). J. Bacteriol. 178:7159-7166.
16. Gooday, G. W. 1990. The ecology of chitin degradation, p. 387-430. In K. C. Marshall (ed.), Advances in microbial ecology. Plenum Press, New York, N.Y.
17. Grinter, N. J. 1983. A broad host range cloning vector transposante to various replicons. Gene 21:133-143.
18. Heim, R., A. B. Cubitt, and R. Y. Tsien. 1995. Improved green fluorescence. Nature 373:663-664.
19. Herrero, M., V. de Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172:6557-6567.
20. Hodson, R. E., W. A. Dustman, R. P. Garg, and A. M. Moran. 1995. In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl. Environ. Microbiol. 61:407-4082.
21. Jacobs, D., M. L. Angles, A. E. Goodman, and B. A. Neilan. 1997. Improved methods for in situ enzymatic amplification and detection of low copy number genes in bacteria. FEMS Microbiol. Lett. 152:65-73.
22. Kalogeraki, V. S., and S. C. Winans. 1997. Suicide plasmids containing promoterless reporter genes can simultaneously disrupt and create fusions to target genes of diverse bacteria. Gene 188:69-75.
23. Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad host-range plasmids for DNA cloning in Gram-negative bacteria. Gene 70:191-197.
24. Kremer, L., A. Baulard, J. Estaquier, O. Poulain-Godefroy, and C. Locht. 1995. Green fluorescent protein as a new expression marker in mycobacteria. Mol. Microbiol. 17:913-922.
25. Labes, M., A. Pühler, and R. Simon. 1990. A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for Gram-negative bacteria. Gene 89:37-46.
26. Leff, L. G., and A. A. Leff. 1996. Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments. Appl. Environ. Microbiol. 62:3486-3488.
27. Matthysse, A. G., S. Stretton, C. Dandie, N. C. McClure, and A. E. Goodman. 1996. Construction of GFP vectors for use in Gram-negative bacteria other than Escherichia coli. FEMS Microbiol. Lett. 145:87-94.
28. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
29. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae toxR. J. Bacteriol. 170:2575-2583.
30. Miller, W. G., and S. E. Lindow. 1997. An improved GFP cloning cassette designed for prokaryotic transcriptional fusions. Gene 191:149-153.
31. Nano, F. E., and S. Kaplan. 1982. Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides. J. Bacteriol. 152:924-927.
32. Östling, J., A. Goodman, and S. Kjelleberg. 1991. Behaviour of IncP-1 plasmids and a miniMu transposon in a marine Vibrio sp.: isolation of starvation inducible lac operon fusions. FEMS Microbiol. Ecol. 86:83-94.
33. Podbielski, A., B. Spellerberg, M. Woischnik, B. Pohl, and R. Lütticken. 1996. Novel series of plasmid vectors for gene inactivation and expression analysis in group A streptococci (GAS). Gene 177:137-147.
34. Poulsen, L. K., H. M. Dalton, M. L. Angles, K. C. Marshall, S. Molin, and A. E. Goodman. 1997. Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures. Appl. Environ. Microbiol. 64:3698-3702.
35. Sambrook, J., E. J. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
36. Sangari, F., and J. Agüero. 1991. Mutagenesis of Brucella abortus: comparative efficiency of three transposon delivery systems. Microb. Pathog. 11:443-446.
37. Stretton, S., K. C. Marshall, I. W. Dawes, and A. E. Goodman. 1996. Characterisation of carbon dioxide-inducible genes of the marine bacterium Pseudomonas sp. S91. FEMS Microbiol. Lett. 140:37-42.
38. Techkarnjanaruk, S., S. Pongpattanakitshote, and A. E. Goodman. 1997. Use of a promoterless lacZ gene insertion to investigate chitinase gene expression in the marine bacterium Pseudoalteromonas sp. strain S9. Appl. Environ. Microbiol. 63:2989-2996.
39. Tolker-Nielsen, T., K. Holstrøm, and S. Molin. 1997. Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR. Appl. Environ. Microbiol. 63:4196-4203.
40. Tombolini, R., A. Unge, M. E. Davey, F. J. de Bruijn, and J. K. Jansson. 1997. Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria. FEMS Microbiol. Ecol. 22:17-28.
41. Valdivia, K. H., A. E. Hromockyj, D. Monack, L. Ramakrishnan, and S. Falkow. 1996. Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene 173:47-52.
42. Webb, C. D., A. Decatur, A. Teleman, and R. Losick. 1995. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. J. Bacteriol. 177:5906-5911.