Localization of xenobiotic-responsive element binding protein in rat hepatocyte nuclei after methylcholanthrene administration as revealed by in situ Southwestern hybridization

Journal of Histochemistry and Cytochemistry

Volumn 46, Issue 7, 1998, Pages 825-832

  Asaka, Youko ^a   Watanabe, Jun ^a   Kanamura, Shinsuke ^a  

^a KANSAI MEDICAL UNIVERSITY   (Japan)

Author keywords

Cytochrome P 450; DNA binding protein; In situ Southwestern hybridization; Liver; Methylcholanthrene; Rat; Xenobiotic responsive element

Indexed keywords

3 METHYLCHOLANTHRENE; BINDING PROTEIN; CYTOCHROME P450;

ANIMAL TISSUE; ARTICLE; DNA FLANKING REGION; ELECTRON MICROSCOPY; IN SITU HYBRIDIZATION; LASER MICROSCOPY; LIVER CELL NUCLEUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; REGULATORY MECHANISM; TISSUE SECTION; TRANSCRIPTION REGULATION;

ANIMALIA; EUKARYOTA;

EID: 0031802464     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549804600705     Document Type: Article

References (24)

1. Bacsi SG, Reisz-Porszasz S, Hankinson O (1995) Orientation of the heterodimeric aryl hydrocarbon (dioxin) receptor complex on its asymmetric DNA recognition sequence. Mol Pharmacol 47: 432-438
2. Baron J, Redick JA, Guengerich FP (1982) Effects of 3-methylcholanthrene, β-naphthoflavone, and phenobarbital on the 3-methylcholanthrene-inducible isozyme of cytochromes P-450 within centrilobular, midzonal, and periportal hepatocytes. J Biol Chem 257:953-957
3. Blobel G, Potter VR (1966) Nuclei from rat liver: isolation method that combines purity with high yield. Science 154:1662-1665
4. Burbach KM, Poland A, Bradfield CA (1992) Cloning of the Ah receptor cDNA reveals a distinctive ligand-activated transcription factor. Proc Natl Acad Sci USA 89:8185-8189
5. Carver LA, Bradfield CA (1997) Ligand-dependent interaction of the aryl hydrocarbon receptor with a novel immunophilin homolog in vivo. J Biol Chem 272:11452-11456
6. Dulley JR, Grieve PA (1975) A simple technique for eliminating interference by detergents in the Lowry method of protein determination. Anal Biochem 64:136-141
7. Fujisawa-Sehara A, Sogawa K, Nishi C, Fujii-Kuriyama Y (1986) Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene. Nucleic Acids Res 14:1465-1477
8. Giannone JV, Okey AB, Harper PA (1995) Characterization of polyclonal antibodies to the aromatic hydrocarbon receptor. Can J Physiol Pharmacol 73:7-17
9. Hankinson O (1995) The aryl hydrocarbon receptor complex. Annu Rev Pharmacol Toxicol 35:307-340
10. Hapgood J, Cuthill S, Denis M, Poelinger L, Gustafsson J-Å (1989) Specific protein-DNA interactions at a xenobiotic-responsive element: copurification of dioxin receptor and DNA-binding activity. Proc Natl Acad Sci USA 86: 60-64
11. Hord NG, Perdew GH (1994) Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator. Mol Pharmacol 46:618-626
12. Jones PBC, Galeazzi DR, Fisher JM, Whitlock JP Jr (1985) Control of cytochrome P1-450 gene expression by dioxin. Science 227:1499-1501
13. Kobayashi A, Sogawa K, Fujii-Kuriyama Y (1996) Cooperative interaction between AhR·Arnt and Sp1 for the drug-inducible expression of CYP1A1 gene. J Biol Chem 271:12310-12316
14. Koji T, Komuta K, Nozawa M, Yamada S, Nakane PK (1994) Localization of cyclic adenosine 3′5′monophosphate-responsive element (CRE)-binding proteins by Southwestern histochemistry. J Histochem Cytochem 42:1399-1405
15. Koji T, Yamada S, Kayashima K, Nakane PK (1992) A new approach to localize glucocorticoid receptor using DNA probe containing glucocorticoid responsive element DNA consensus sequence. Acta Histochem Cytochem 25:681-687
16. Ma Q, Whitlock JP Jr (1997) A novel cytoplasmic protein that interacts with the Ah receptor, contains tetratncopeptide repeat motifs, and augments the transcriptional response to 2,3,7,8-tetrachlorodibenzo-p-dioxin. J Biol Chem 272:8878-8884
17. Pollenz RS (1996) The aryl hydrocarbon receptor, but not the aryl hydrocarbon receptor nuclear translocator protein, is rapidly depleted in hepatic and nonhepatic cultured cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Mol Pharmacol 49:391-398
18. Pollenz RS, Sattler CR, Poland A (1993) The aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein show distinct subcellular localizations in Hepa 1c1c7 cells by immunofluorescence microscopy. Mol Pharmacol 45:428-438
19. Probst MR, Reisz-Porszasz S, Agbunag RV, Ong MS, Hankinson O (1993) Role of the aryl hydrocarbon receptor nuclear translocator protein in aryl hydrocarbon (dioxin) receptor action. Mol Pharmacol 44:511-518
20. Reisz-Porszasz S, Probst MR, Fukunaga BN, Hankinson O (1994) Identification of functional domain of the aryl hydrocarbon receptor nuclear translocator protein (Arnt). Mol Cell Biol 14: 6075-6086
21. Swanson HI, Chan WK, Bradfield CA (1995) DNA binding specificities and pairing rules of the Ah receptor, ARNT, and SIM proteins. J Biol Chem 270:26292-26302
22. Tanaka T, Watanabe J, Asaka Y, Ogawa R, Kanamura S (1997) Quantitative analysis of endoplasmic reticulum and cytochrome P-450 in hepatocytes from rats injected with methylcholanthrene. Eur J Cell Biol 74:20-30
23. Watanabe J, Asaka Y, Kanamura S (1996) Relationship between immunostaining intensity and antigen content in sections. J Histochem Cytochem 44:1451-1458
24. Whitlock JP Jr, Okino ST, Dong L, Ko HP, Clarke-Katzenberg R, Ma Q, Li H (1996) Induction of cytochrome P-4501A1: a model for analyzing mammalian gene transcription. FASEB J 10:809-818