-
1
-
-
0015217635
-
Simultaneous formation of two alternative enzymology active structures by complementation of two overlapping fragments of staphylococcal nuclease
-
Taniuchi, H. and Anfinsen, C.B. (1971) Simultaneous formation of two alternative enzymology active structures by complementation of two overlapping fragments of staphylococcal nuclease. J. Biol. Chem. 246, 2291-2301
-
(1971)
J. Biol. Chem.
, vol.246
, pp. 2291-2301
-
-
Taniuchi, H.1
Anfinsen, C.B.2
-
2
-
-
0029007891
-
Complementation of fragments of triosephosphate isomerase defined by exon boundaries
-
Bertolaet, B.L. and Knowles, J.R. (1995) Complementation of fragments of triosephosphate isomerase defined by exon boundaries. Biochemistry 34, 5736-5743
-
(1995)
Biochemistry
, vol.34
, pp. 5736-5743
-
-
Bertolaet, B.L.1
Knowles, J.R.2
-
3
-
-
0031043161
-
Nucleation mechanisms in protein folding
-
Fersht, A.R. (1997) Nucleation mechanisms in protein folding. Curr. Opin. Struct. Biol. 7, 3-9
-
(1997)
Curr. Opin. Struct. Biol.
, vol.7
, pp. 3-9
-
-
Fersht, A.R.1
-
4
-
-
0027240033
-
Topological and stereochemical restrictions in betasandwich protein structures
-
Woolfson, D.N., Evans, P.A., Hutchinson, E.G., and Thornton, J.M. (1993) Topological and stereochemical restrictions in betasandwich protein structures. Protein Eng. 6, 461-470
-
(1993)
Protein Eng.
, vol.6
, pp. 461-470
-
-
Woolfson, D.N.1
Evans, P.A.2
Hutchinson, E.G.3
Thornton, J.M.4
-
5
-
-
0031576337
-
Glutamine, alanine or glycine repeats inserted into the loop of a protein have minimal effects on stability and folding rates
-
Ladurner, A.G. and Fersht, A.R. (1997) Glutamine, alanine or glycine repeats inserted into the loop of a protein have minimal effects on stability and folding rates. J. Mol. Biol. 273, 330-337
-
(1997)
J. Mol. Biol.
, vol.273
, pp. 330-337
-
-
Ladurner, A.G.1
Fersht, A.R.2
-
6
-
-
0031019924
-
The folding pathway of a protein at high resolution from microseconds to seconds
-
Nolting, B., Golbik, R., Neira, J.L., Soler-Gonzalez, A.S., Schreiber, G., and Fersht, A.R. (1997) The folding pathway of a protein at high resolution from microseconds to seconds. Proc. Natl. Acad. Sci. USA 94, 826-830
-
(1997)
Proc. Natl. Acad. Sci. USA
, vol.94
, pp. 826-830
-
-
Nolting, B.1
Golbik, R.2
Neira, J.L.3
Soler-Gonzalez, A.S.4
Schreiber, G.5
Fersht, A.R.6
-
7
-
-
0001079105
-
On the use of sequence homologies to predict protein structure: Identical pentapeptides can have completely different conformations
-
Kabsch, W. and Sander, C. (1984) On the use of sequence homologies to predict protein structure: identical pentapeptides can have completely different conformations. Proc. Natl. Acad. Sci. USA 81, 1075-1078
-
(1984)
Proc. Natl. Acad. Sci. USA
, vol.81
, pp. 1075-1078
-
-
Kabsch, W.1
Sander, C.2
-
8
-
-
0026030641
-
Database of homology-derived protein structures and the structural meaning of sequence alignment
-
Sander, C. and Schneider, R. (1991) Database of homology-derived protein structures and the structural meaning of sequence alignment. Proteins 9, 56-68
-
(1991)
Proteins
, vol.9
, pp. 56-68
-
-
Sander, C.1
Schneider, R.2
-
9
-
-
0025183708
-
Basic local alignment search tool
-
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol Biol. 215, 403-410
-
(1990)
J. Mol Biol.
, vol.215
, pp. 403-410
-
-
Altschul, S.F.1
Gish, W.2
Miller, W.3
Myers, E.W.4
Lipman, D.J.5
-
10
-
-
0000899457
-
Dihydrofolate reductase
-
Blakley, R.L. and Benkovic, S.J., eds. Wiley, New York
-
Blakley, R.L. (1984) Dihydrofolate reductase in Folates and Pteridines (Blakley, R.L. and Benkovic, S.J., eds.) Vol. 1, pp. 191-253, Wiley, New York
-
(1984)
Folates and Pteridines
, vol.1
, pp. 191-253
-
-
Blakley, R.L.1
-
11
-
-
0025040496
-
Chemo-enzymatic synthesis of optically pure Meucovorin, an augmentor of 5-fluorouracil cytotoxicity against cancer
-
Uwajima, T., Oshiro, T., Eguchi, T., Kuge, Y., Horiguchi, A., Igarashi, A., Mochida, K., and Iwakura, M. (1990) Chemo-enzymatic synthesis of optically pure Meucovorin, an augmentor of 5-fluorouracil cytotoxicity against cancer. Biochem. Biophys. Res. Commun. 171, 684-689
-
(1990)
Biochem. Biophys. Res. Commun.
, vol.171
, pp. 684-689
-
-
Uwajima, T.1
Oshiro, T.2
Eguchi, T.3
Kuge, Y.4
Horiguchi, A.5
Igarashi, A.6
Mochida, K.7
Iwakura, M.8
-
12
-
-
0026065644
-
Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding
-
Bystroff, C. and Kraut, J. (1991) Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding. Biochemistry 30, 2227-2239
-
(1991)
Biochemistry
, vol.30
, pp. 2227-2239
-
-
Bystroff, C.1
Kraut, J.2
-
13
-
-
0021024041
-
Directed mutagenesis of dihydrofolate reductase
-
Villafranca, J.E., Howell, E.E., Voet, D.H., Strobel, M.S., Ogden, R.C., Abelson, J.N., and Kraut, J. (1983) Directed mutagenesis of dihydrofolate reductase. Science 222, 782-788
-
(1983)
Science
, vol.222
, pp. 782-788
-
-
Villafranca, J.E.1
Howell, E.E.2
Voet, D.H.3
Strobel, M.S.4
Ogden, R.C.5
Abelson, J.N.6
Kraut, J.7
-
14
-
-
0023240190
-
Effect of single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli
-
Perry, K.M., Onuffer, J.J., Touchette, N.A., Herndon, C.S., Gittelman, M.S., Matthews, C.R., Chen, J.T., Mayer, K.J., Taira, K., and Benkovic, S. J. (1987) Effect of single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli. Biochemistry 26, 2674-2682
-
(1987)
Biochemistry
, vol.26
, pp. 2674-2682
-
-
Perry, K.M.1
Onuffer, J.J.2
Touchette, N.A.3
Herndon, C.S.4
Gittelman, M.S.5
Matthews, C.R.6
Chen, J.T.7
Mayer, K.J.8
Taira, K.9
Benkovic, S.J.10
-
15
-
-
0024784280
-
A hydrophobic cluster forms early in the folding of dihydrofolate reductase
-
Garvey, E.P., Swank, J., and Matthews, C.R. (1989) A hydrophobic cluster forms early in the folding of dihydrofolate reductase. Proteins 6, 259-266
-
(1989)
Proteins
, vol.6
, pp. 259-266
-
-
Garvey, E.P.1
Swank, J.2
Matthews, C.R.3
-
16
-
-
0025076417
-
Dihydrofolate reductase from Escherichia coli: Probing the role of aspartate-27 and phenylalanine-137 in enzyme conformation and the binding of NADPH
-
Dunn, S.M., Lanigan, T.M., and Howell, E.E. (1990) Dihydrofolate reductase from Escherichia coli: probing the role of aspartate-27 and phenylalanine-137 in enzyme conformation and the binding of NADPH. Biochemistry 29, 8569-8576
-
(1990)
Biochemistry
, vol.29
, pp. 8569-8576
-
-
Dunn, S.M.1
Lanigan, T.M.2
Howell, E.E.3
-
17
-
-
0027408870
-
Comparative stability of dihydrofolate reductase mutants in vitro and in vivo
-
Leontiev, V.V., Uversky, V.N., and Gudkov, A.T. (1993) Comparative stability of dihydrofolate reductase mutants in vitro and in vivo. Protein Eng. 6, 81-84
-
(1993)
Protein Eng.
, vol.6
, pp. 81-84
-
-
Leontiev, V.V.1
Uversky, V.N.2
Gudkov, A.T.3
-
18
-
-
0028952263
-
A strategy for testing the suitability of cysteine replacements in dihydrofolate reductase from Escherichia coli
-
Iwakura, M., Jones, B.E., Luo, J., and Matthews, C.R. (1995) A strategy for testing the suitability of cysteine replacements in dihydrofolate reductase from Escherichia coli. J. Biochem. 117, 480-488
-
(1995)
J. Biochem.
, vol.117
, pp. 480-488
-
-
Iwakura, M.1
Jones, B.E.2
Luo, J.3
Matthews, C.R.4
-
19
-
-
0031937819
-
Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of Escherichia coli dihydrofolate reductase on its stability and function
-
Ohmae, E., Iriyama, K., Ichihara, S., and Gekko, K. (1998) Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of Escherichia coli dihydrofolate reductase on its stability and function. J. Biochem. 123, 33-41
-
(1998)
J. Biochem.
, vol.123
, pp. 33-41
-
-
Ohmae, E.1
Iriyama, K.2
Ichihara, S.3
Gekko, K.4
-
20
-
-
0026516388
-
A fully active variant of dihydrofolate reductase with a circularly permuted sequence
-
Buchwalder, A., Szadkowski, H., and Kirschner, K. (1992) A fully active variant of dihydrofolate reductase with a circularly permuted sequence. Biochemistry 31, 1621-1630
-
(1992)
Biochemistry
, vol.31
, pp. 1621-1630
-
-
Buchwalder, A.1
Szadkowski, H.2
Kirschner, K.3
-
21
-
-
0028173592
-
Circularly permuted dihydrofolate reductase of E. coli has functional activity and a destabilized tertiary structure
-
Protasova, N.Yu, Kireeva, M.L., Murzina, N.V., Murzin, A.G., Uversky, V.N., Gryaznova, O.I., and Gudkov, A.T. (1994) Circularly permuted dihydrofolate reductase of E. coli has functional activity and a destabilized tertiary structure. Protein Eng. 7, 1373-1377
-
(1994)
Protein Eng.
, vol.7
, pp. 1373-1377
-
-
Protasova, N.Yu.1
Kireeva, M.L.2
Murzina, N.V.3
Murzin, A.G.4
Uversky, V.N.5
Gryaznova, O.I.6
Gudkov, A.T.7
-
22
-
-
0031815941
-
Effects of the length of a glycine linker connecting the N- and C-termini of a circularly permuted dihydrofolate reductase
-
Iwakura, M. and Nakamura, T. (1998) Effects of the length of a glycine linker connecting the N-and C-termini of a circularly permuted dihydrofolate reductase. Protein Eng. 11, 707-713
-
(1998)
Protein Eng.
, vol.11
, pp. 707-713
-
-
Iwakura, M.1
Nakamura, T.2
-
23
-
-
0040768065
-
-
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
-
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
-
(1982)
Molecular Cloning. A Laboratory Manual
-
-
Maniatis, T.1
Fritsch, E.F.2
Sambrook, J.3
-
24
-
-
0026502870
-
Dihydrofolate reductase as a new "affinity handle."
-
Iwakura, M., Furusawa, K., Kokubu, T., Ohashi, S., Shimura, Y., and Tsuda, K. (1992) Dihydrofolate reductase as a new "affinity handle." J. Biochem. III, 37-45
-
(1992)
J. Biochem.
, vol.3
, pp. 37-45
-
-
Iwakura, M.1
Furusawa, K.2
Kokubu, T.3
Ohashi, S.4
Shimura, Y.5
Tsuda, K.6
-
25
-
-
0014949207
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
-
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
-
(1970)
Nature
, vol.227
, pp. 680-685
-
-
Laemmli, U.K.1
-
26
-
-
0023055739
-
Folding of dihydrofolate reductase from Escherichia coli
-
Touchette, N.A., Perry, K.P., and Matthews, C.R. (1986) Folding of dihydrofolate reductase from Escherichia coli. Biochemistry 25, 5445-5452
-
(1986)
Biochemistry
, vol.25
, pp. 5445-5452
-
-
Touchette, N.A.1
Perry, K.P.2
Matthews, C.R.3
-
27
-
-
0014151463
-
Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase
-
Hillcoat, B., Nixon, P., and Blakely, R.L. (1967) Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase. Anal. Biochem. 21, 178-189
-
(1967)
Anal. Biochem.
, vol.21
, pp. 178-189
-
-
Hillcoat, B.1
Nixon, P.2
Blakely, R.L.3
-
28
-
-
0023748631
-
Mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli: PH and deuterium isotope effects with NADPH as the variable substrate
-
Morrison, J.F. and Stone, S.R. (1988) Mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli: pH and deuterium isotope effects with NADPH as the variable substrate. Biochemistry 27, 5499-5506
-
(1988)
Biochemistry
, vol.27
, pp. 5499-5506
-
-
Morrison, J.F.1
Stone, S.R.2
-
29
-
-
0023697408
-
Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants
-
Santoro, M.M. and Bolen, D.W. (1988) Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants. Biochemistry 27, 8063-8068
-
(1988)
Biochemistry
, vol.27
, pp. 8063-8068
-
-
Santoro, M.M.1
Bolen, D.W.2
-
30
-
-
0027050469
-
Construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains
-
Iwakura, M. and Matthews, C.R. (1993) Construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains. Protein Eng. 5, 791-796
-
(1993)
Protein Eng.
, vol.5
, pp. 791-796
-
-
Iwakura, M.1
Matthews, C.R.2
-
31
-
-
0029129147
-
Production of hetero-dimeric dihydrofolate reductase-thymidylate synthase bifunctional enzyme
-
Iwakura, M. and Kokubu, T. (1995) Production of hetero-dimeric dihydrofolate reductase-thymidylate synthase bifunctional enzyme. J. Biochem. 118, 67-74
-
(1995)
J. Biochem.
, vol.118
, pp. 67-74
-
-
Iwakura, M.1
Kokubu, T.2
-
32
-
-
0026005997
-
Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy
-
Kuwajima, K., Garvey, E.P., Finn, B.E., Matthews, C.R., and Sugai, S. (1991) Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy. Biochemistry 30, 7693-7703
-
(1991)
Biochemistry
, vol.30
, pp. 7693-7703
-
-
Kuwajima, K.1
Garvey, E.P.2
Finn, B.E.3
Matthews, C.R.4
Sugai, S.5
-
33
-
-
0026596758
-
Ordered self-assembly of polypeptide fragments to form nativelike dimeric trp repressor
-
Tasayco, M.L. and Carey, J. (1992) Ordered self-assembly of polypeptide fragments to form nativelike dimeric trp repressor. Science 255, 594-597
-
(1992)
Science
, vol.255
, pp. 594-597
-
-
Tasayco, M.L.1
Carey, J.2
-
34
-
-
0027442944
-
A noncovalent peptide complex as a model for an early folding intermediate of cytochrome c
-
Wu, L.C., Laub, P.B., Elove, G.A., Carey, J., and Roder, H. (1993) A noncovalent peptide complex as a model for an early folding intermediate of cytochrome c. Biochemistry 32, 10271-10276
-
(1993)
Biochemistry
, vol.32
, pp. 10271-10276
-
-
Wu, L.C.1
Laub, P.B.2
Elove, G.A.3
Carey, J.4
Roder, H.5
-
35
-
-
0028212643
-
Autonomous subdomains in protein folding
-
Wu, L.C., Grandori, R., and Carey, J. (1994) Autonomous subdomains in protein folding. Protein Sci. 3, 369-371
-
(1994)
Protein Sci.
, vol.3
, pp. 369-371
-
-
Wu, L.C.1
Grandori, R.2
Carey, J.3
-
36
-
-
0028334906
-
A protein dissection study of a molten globule
-
Peng, Z.Y. and Kim, P.S. (1994) A protein dissection study of a molten globule. Biochemistry 33, 2136-2141
-
(1994)
Biochemistry
, vol.33
, pp. 2136-2141
-
-
Peng, Z.Y.1
Kim, P.S.2
-
37
-
-
0030755307
-
Probing minimal independent folding units in dihydrofolate reductase by molecular dissection
-
Gegg, C.V., Bowers, K.E., and Matthews, C.R. (1997) Probing minimal independent folding units in dihydrofolate reductase by molecular dissection. Protein Sci. 6, 1885-1892
-
(1997)
Protein Sci.
, vol.6
, pp. 1885-1892
-
-
Gegg, C.V.1
Bowers, K.E.2
Matthews, C.R.3
-
38
-
-
0030605814
-
Linguistic analysis of protein folding
-
Gross, M. (1996) Linguistic analysis of protein folding. FEBS Lett. 390, 249-252
-
(1996)
FEBS Lett.
, vol.390
, pp. 249-252
-
-
Gross, M.1
-
39
-
-
0026782888
-
Exons as microgenes?
-
Seidel, H.M., Pompliano, D.L., and Knowles, J.R. (1992) Exons as microgenes? Science 257, 1489-1490
-
(1992)
Science
, vol.257
, pp. 1489-1490
-
-
Seidel, H.M.1
Pompliano, D.L.2
Knowles, J.R.3
-
40
-
-
15144359191
-
An intramolecular shuffling of secondary structural motifs in E. coli dihydrofolate reductase yields an active enzyme
-
Iwakura, M. (1996) An intramolecular shuffling of secondary structural motifs in E. coli dihydrofolate reductase yields an active enzyme. Protein Sci. 5, Suppl. 1, 68
-
(1996)
Protein Sci.
, vol.5
, Issue.1 SUPPL.
, pp. 68
-
-
Iwakura, M.1
|