Expression and secretion of Trichoderma endoglucanase in Saccharomyces cerevisiae

Korean Journal of Applied Microbiology and Biotechnology

Volumn 26, Issue 5, 1998, Pages 406-412

  Shin, Dong Ha ^a   Kim, Jae Bum ^a   Kim, Byung Woo ^a   Nam, Soo Wan ^a   Shin, Ji Won ^a,b   Chung, Dae Kyun ^a,b   Jeong, Choon Soo ^a,c  

^a Dong Eui University   (South Korea)

^b Kyung Hee University   (South Korea)

^c University of Ulsan   (South Korea)

Author keywords

ADH1 promoter; Expression; Glycosylation; Saccharomyces cerevisiae; Trichoderma endoglucanase

Indexed keywords

GLUCAN SYNTHASE; RECOMBINANT ENZYME;

ARTICLE; BACTERIAL GROWTH; BACTERIUM TRANSFORMATION; ENZYME LOCALIZATION; ENZYME RELEASE; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; SACCHAROMYCES CEREVISIAE; TRICHODERMA;

SACCHAROMYCES CEREVISIAE; TRICHODERMA;

EID: 0031790218     PISSN: 02572389     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Barnett, C. C., R. M. Berka, and T. Fowler. 1991. Cloning and amplification of the gene encoding an extracellular β-glucosidase from Trichoderma reesei: Evidence for improved rates of saccharification of cellulosic substrates. Bio/Technol. 9: 562-567.
2. Broker, M., O. Bauml, A. Gottig, J. Ochs, M. Bodenbenner, and E. Amann. 1991. Expression of the human blood coagulation protein factor XIIIa in Saccharomyces cerevisiae: Dependence of the expression levels from host-vector systems and medium conditions. Appl. Microbiol Biotechnol. 34: 756-764.
3. Chen, C. M., M. Gritzali, and D. W. Stafford. 1987. Nucleotide sequence and deduced primary structure of cellobiohydrolase II from Trichoderma reesei. Bio/Technol. 5: 274-278.
4. Chung, D. K., D. H. Shin, B. W. Kim, J. K. Nam, I. S. Han, and S. W. Nam. 1997. Expression and secretion of Clostridium thermocellum endoglucanase A gene (celA) in different Saccharomyces cerevisiae strains. Biotechnol. Lett. 19: 503-508.
5. Draborg, H., S. Christgau, T. Halkier, G. Rasmussen, H. Dalboge, and S. Kauppinen. 1996. Secretion of an enzymatically active Trichoderma harzianum endochitinase by Saccharomyces cerevisiae. Curr. Genet. 29: 404-409.
6. Hardjito, L., P. F. Greenfleld, and P. L. Lee. 1993. Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae. Enzyme Microbiol. Technol. 15: 120-126.
7. Henrissat, B., H. Driguez, C. Viet, and M. Schulein. 1985. Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Bio/Technol. 3: 722-726.
8. Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells with alkali cations. J. Bacteriol. 153: 163-168.
9. Klarskov, K., K. Piens, J. Stahlberg, P. B. Hoj, J. Van Beeumen, and M. Claeyssens. 1997. Cellobiohydrolase I from Trichoderma reesei: Identification of an active-site nucleophile and additional information on sequence including the glycosylation pattern of the core protein. Carbohydr. Res. 304: 143-154.
10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
11. La Grange, D. C., I. S. Pretorius, and W. H. van Zyl. 1996. Expression of a Trichoderma reesei β-xylanase (XYN2) in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 62: 1036-1044.
12. Maras, M., A. De Bruyn, J. Schraml, P. Herdewijn, M. Claeyssens, W. Fiers, and R. Contreras. 1997. Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30. Eur. J. Biochem. 245: 617-625.
13. Margolles-Clark, E., M. Tenkanen, T. Nakari-Setala, and M. Penttila. 1996. Cloning of genes encoding α-L-arabinofuranosidase and β-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae. Appl. Environ. Microbiol 62: 3840-3846.
14. Miller, G. L., R. Blum, W. E. Glennon, and A. L. Burton. 1960. Measurement of carboxymethyl-cellulase activity. Anal. Biochem. 2: 127-132.
15. Nam, S. W., K. Yoda, and M. Yamasaki. 1993. Secretion and localization of invertase and inulinase in recombinant Saccharomyces cerevisiae. Biotechnol. Lett. 15: 1049-1054.
16. Nevalainen, H. and M. Penttila. 1995. Molecular biology of cellulolytic fungi, pp. 303-319. In U. Kuck(ed.), The Mycota II. Genetics and Biotechnology. Springer-Verlag, Berlin.
17. Park, Y. S., S. Shiba, S. Iijima, T. Kobayashi, and F. Hishinuma. 1993. Comparison of three different promoter systems for secretory α-amylase production in fed-batch cultures of recombinant Saccharomyces cerevisiae. Biotechnol Bioeng. 41: 854-861.
18. Penttila, M., P. Lehtovaara, H. Nevalainen, R. Bhikhabhai, and J. K. C. Knowles. 1986. Homology between cellulases genes of Trichoderma reesei: Complete nucleotide sequence of the endoglucanase I gene. Gene 45: 253-263.
19. Penttila, M., L. Andre, M. Saloheimo, P. Lehtovaara, and J. K. C. Knowles. 1987. Expression of two Trichoderma reesei endoglucanase in the yeast Saccharomyces cerevisiae. Yeast 3: 175-185.
20. Penttila, M., L. Andre, P. Lehtovaara, M. Bailey, T. T. Teeri, and J. K. C. Knowles. 1988. Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae. Gene 63: 103-112.
21. Saloheimo, M., P. Lehtovaara, M. Penttila, T. T. Teeri, J. Stalberg, G. Johansson, G. Pettersson, M. Claeyssens, P. Tomme, and J. K. C. Knowles. 1988. EGIII, a new endoglucanase from Trichoderma reesei: The characterization of both gene and enzyme. Gene 63: 11-21.
22. Saloheimo, A., B. Henrissat, A. -M. Hoffren, O. Teleman, and M. Penttila. 1994. A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast. Mol. Microbiol. 13: 219-228.
23. Saloheimo, M., T. Nakari-Setala, M. Tenkanen, and M. Penttila. 1997. cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast. Eur. J. Biochem. 249: 584-591.
24. Salovuori, I., M. Makarow, H. Rauvala, J. K. C. Knowles, and L. Kaariainen. 1987. Low molecular mass high-mannose type glycans in a secreted protein of the filamentous fungus Trichoderma reesei. Bio/Technol. 5: 152-156.
25. Schultz, L., H. Z. Markus, K. J. Hofmann, D. L. Montgomery, C. T. Dunwiddie, P. J. Kniskern, R. B. Freedman, R. W. Ellis, and M. F. Tuite. 1994. Using molecular genetics to improve the production of recombinant proteins by the yeast Saccharomyces cerevisiae. Ann. NY Acad. Sci. 721: 148-157.
26. Shoemaker, S., V. Schweickart, M. Ladner, D. Gelfand, S. Kwok, K. Myambo, and M. Innis. 1983. Molecular cloning of exo-cellobiohydrolase derived from Trichoderma reesei strain L27. Bio/Technol. 1: 691-695.
27. Shuster, J. R. 1991. Gene expression in yeast: Protein secretion. Curr. Opinion Biotechnol. 2: 685-690.
28. Son, Y. J., O. J. Sul, D. K. Chung, I. S. Han, Y. J. Choi, and C. S. Jeong. 1997. Isolation and characterization of Trichoderma sp. C-4 producing cellulases. Kor. J. Appl. Microbiol. Biotechnol. 25: 346-353.
29. Stalbrand, H., A. Saloheimo, J. Vehmaanpera, B. Henrissat, and M. Penttila. 1995. Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei β-mannanase gene containing a cellulose binding domain. Appl. Environ. Microbiol. 61: 1090-1097.
30. Teeri, T. T., I. Salovuori, and J. K. C. Knowles. 1983. The molecular cloning of the major cellulase gene from Trichoderma reesei. Bio/Technol. 1: 696-699.
31. Teeri, T. T., P. Lehtovaara, S. Kauppinen, I. Salovuori, and J. K. C. Knowles. 1983. Homologous domains in Trichoderma reesei celluolytic enzymes: Gene sequence and expression of cellobiohydrolase II. Gene 51: 43-52.
32. Tomme, P., R. A. J. Warren, and N. R. Gilkes. 1995. Cellulose hydrolysis by bacteria and fungi. Adv. Microbiol. Physiol 37: 1-81.
33. Van Arsdell, J. N., S. Kwok, V. L. Schweickart, M. B. Ladner, D. H. Gelfand, and M. A. Innis. 1987. Cloning, characterization, and expression in Saccharomyces cerevisiae of endoglucanase I from Trichoderma reesei. Bio/ Technol. 5: 60-64.
34. Vernet, T., D. Dignard, and D. Y. Thomas. 1987. A family of yeast expression vectors containing the phage f1 intergenic region. Gene 52: 225-233.
35. Ward, M., S. Wu, J. Dauberman, G. Weiss, E. Larenas, B. Bower, M. Rey, K. Clarkson, and R. Bott. 1993. Cloning, sequence and preliminary structural analysis of a small, high pI endoglucanase (EGIII) from Trichoderma reesei, pp. 153-158. In P. Suominen and T. Reinikainen (eds.), Proceedings of the Second TRICEL Symposium Trichoderma reesei cellulases and other hydrolases Foundation for Biotechnical and Industrial Fermentation Research. Helsinki.
36. Woodward, J., M. K. Hayes, and N. E. Lee. 1988. Hydrolysis of cellulose by saturating and non-saturating concentrations of cellulase: Implications for synergism. Bio/ Technol. 6: 301-304.