Differential polymerase chain reaction: A technical comparison of three methods for the detection of CDK4 gene amplification in glioblastomas

International Journal of Oncology

Volumn 13, Issue 5, 1998, Pages 963-966

  Fan, X ^a   Gomez L ^a   Nistal, M ^b   Sierrasesumaga L ^a   Castresana, J S ^a  

^a UNIVERSITY OF NAVARRA   (Spain)

^b UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

CDK4; Differential PCR; Gene amplification; Glioblastoma

Indexed keywords

DNA; ETHIDIUM BROMIDE; SILVER;

ARTICLE; CONTROLLED STUDY; DENSITOMETRY; FLUORESCENCE; GENE AMPLIFICATION; GLIOBLASTOMA; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; ONCOGENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SILVER STAINING; SOUTHERN BLOTTING;

EID: 0031764974     PISSN: 10196439     EISSN: None     Source Type: Journal    
DOI: 10.3892/ijo.13.5.963     Document Type: Article

References (12)

1. Alitalo K and Schwab M: Oncogene amplification in tumor cells. Adv Cancer Res 47: 235-281, 1986.
2. Brodeur GM, Seeger RC, Schwab M, Varmus HE and Bishop JM: Amplification of N-myc in untreated human neuroblastoma correlated with advance disease stage. Science 244: 1121-1124, 1984.
3. Borg A, Tandon AK, Sigurdsson H, Clark GM, Ferno M, Fuqua SAW, Killander D and McGuire WL: Her-2/neu amplification predicts poor survival in node-positive breast cancer. Cancer Res 50: 4332-4337, 1990.
4. Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503-517, 1975.
5. Ueki K, Ono Y, Henson JW, Efird JT, von Deimling A and Louis DN: CDKN2/p16 or RB alterations occur in the majority of glioblastomas and arc inversely correlated. Cancer Res 56: 150-153, 1996.
6. Waha A, Rollbrocker B, Wiestler OD and von Deimling A: A polymerase chain reaction-based assay for the rapid detection of gene amplification in human tumors. Diagn Mol Pathol 5: 147-150, 1996.
7. Kyomoto R, Kumazawa H, Toda Y, Sakaida N, Okamura A, Iwanaga M, Shintaku M, Yamashita T, Hiai H and Fukumoto M: Cyclin-D1-gene amplification is a more potent prognostic factor than its protein overexpression in human head-andneck squamous-cell carcinoma. Int J Cancer 74: 576-581, 1997.
8. Rollbrocker B, Waha A, Louis D, Wiestler OD and von Deimling A: Amplification of the cyclin-dependent kinase (CDK4) gene is associated with high cdk4 protein levels in glioblastoma multiforme. Acta Neuropathol 92: 70-74, 1996.
9. Sambrook J, Fritsch EF and Maniatis T: Molecular cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
10. Hiort O, Wodtke A, Struve D, Zölner A, Sinnecker GHG, et al: Detection of point mutations in the androgen receptor gene using non-isotopic single strand conformation polymorphism analysis. Hum Mol Genet 3: 1163-1166, 1994.
11. Reiferberger G, Reiferberger J, Ichimura K, Meltzer PS and Collins VP: Amplification of multiple genes from chromosomal region 12q13-q14 in human malignant gliomas: preliminary mapping of the amplicons shows preferential involvement of CDK4, SAS and MDM2. Cancer Res 54: 4299-4303, 1994.
12. An H-X, Niederacher D, Beckmann MW, Göhrin U-J, Scharl A, Picard F, van Roeyen C, Schnürch H-G and Bender HG: ERBB2 gene amplification detected by fluorescent differential polymerase chain reaction in paraffin-embedded breast carcinoma tissues. Int J Cancer 64: 291-297, 1995.