Severing of F-actin by the amino-terminal half of gelsolin suggests internal cooperativity in gelsolin

Biophysical Journal

Volumn 75, Issue 6, 1998, Pages 3092-3100

  Selden, Lynn A ^a   Kinosian, Henry J ^b   Newman, Jay ^c   Lincoln, Bryan ^c   Hurwitz, Charles ^a   Gershman, Lewis C ^a,b   Estes, James E ^a,b  

^a STRATTON VA MEDICAL CENTER   (United States)

^b ALBANY MEDICAL COLLEGE   (United States)

^c UNION COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN BINDING PROTEIN; CALCIUM ION; F ACTIN; GELSOLIN; MAGNESIUM ION; PHALLOIDIN;

ACTIN FILAMENT; ANIMAL TISSUE; ARTICLE; BINDING SITE; DOWN REGULATION; LIGHT SCATTERING; NONHUMAN; RABBIT; SKELETAL MUSCLE;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0031738101     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(98)77750-1     Document Type: Article

References (28)

1. 2+ affect the rate at which gelsolin severs F-actin. J. Biol. Chem. 269:32916-32923.
2. Asch, H. L., K. Head, Y. Dong, F. Natoli, J. S. Winston, J. L. Connolly, and B. B. Asch. 1996. Widespread loss of gelsolin in breast cancers of humans, mice, and rats. Cancer Res. 56:4841-4845.
3. 2+ regulatory domain in human brevin. J. Cell Biol. 102:1439-1446.
4. Burtnick, L. D., E. K. Koepf, J. Grimes, E. Y. Jones, D. I. Stuart, P. J. McGlaughlin, and R. C. Robinson. 1997. The crystal structure of plasma gelsolin: implications for actin severing, capping and nucleation. Cell. 90:661-670.
5. Chaponnier, C., P. A. Janmey, and H. L. Yin. 1986. The actin filament severing domain of plasma gelsolin. J Cell Biol. 103:1473-1481.
6. 2+ binding sites of gelsolin that regulate association with actin. Eur. J. Biochem. 229: 512-516.
7. Haddad, J. G., K. D. Harper, M. Guoth, G. G. Pietra, S. W. Sanger. 1990. Angiopathic consequences of saturating the plasma scavenger system for actin. Proc. Natl. Acad. Sci. U.S.A. 87:1381-1385.
8. 2+-induced conformational change of gelsolin is located in the carboxyl-terminal half of the molecule. Biophys. J. 65:799-805.
9. 2+ on protein conformation. Biochemistry. 24:5653-5660.
10. 2+ regulation of gelsolin activity: binding and severing of F-actin. Biophys. J. 75:3101-3109.
11. Kinosian H. J., L. A. Selden, J. E. Estes, and L. C. Gershman. 1993. Actin filament annealing in the presence of ATP and phalloidin. Biochemistry. 32:12353-12357.
12. Kinosian, H. J., L. A. Selden, J. E. Estes, and L. C. Gershman. 1996. Kinetics of gelsolin interaction with phalloidin-stabilized F-actin: rate constants for binding and severing. Biochemistry. 35:16550-16556.
13. Kothakota, S., T. Azuma, C. Reinhard, A. Klippel, J. Tang, K. Chu, T. J. McGarry, M. W. Kirschner, K. Koths, D. J. Kwiatkowski, and L. T. Williams. 1997. Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis. Science. 278:294-298.
14. Kurth, M. C., L.-L. Wang, J. Dingus, and J. Bryan. 1983. Purification and characterization of a gelsolin-actin complex from human platelets. J. Biol. Chem. 258:10895-10903.
15. Kwiatkowski, D. J., P. A. Janmey, and H. L. Yin. 1989. Identification of critical functional and regulatory domains in gelsolin. J. Cell Biol. 108:1717-1726.
16. 2+ requirement. J. Biol. Chem. 268:8999-9004.
17. Lincoln, B., P. L. San Bagio, D. Bulone, J. Newman, C. Hurwitz, H. J. Kinosian, J. E. Estes, and L. C. Gershman. 1998. Actin filaments are structurally weakened by binding of the N-terminal (S1-3) portion of gelsolin. Biophys J. 74:A48.
18. McGough, A., W. Chiu, and M. Way. 1998. Determination of the gelsolin binding site on F-actin: implications for severing and capping. Biophys. J. 74:764-772.
19. McLaughlin, P. J., J. T. Gooch, H. G. Mannherz, and A. G. Weeds. 1993. Structure of gelsolin segment 1-actin complex and the mechanism of filament severing. Nature. 364:685-692.
20. Perrin, D. D., and I. G. Sayce. 1967. Computer calculation of equilibrium concentrations in mixtures of metal ions and complexing species. Talanta. 14:833-842.
21. Pope, B. J., J. T. Gooch, and A. G. Weeds. 1997. Probing the effects of calcium on gelsolin. Biochemistry. 36:15848-15855.
22. Vandekerckhove, J., G. Hauw, K. Vancompernolle, B. Honore, and J. Celis. 1990. Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells. J. Cell Biol. 111:95-102.
23. Vasconcellos, C. A., and S. E. Lind. 1993. Coordinated inhibition of actin-induced platelet aggregation by plasma gelsolin and vitamin D-binding protein. Blood. 82:3648-3657.
24. Way, M., J. Gooch, B. Pope, and A. G. Weeds. 1989. Expression of human plasma gelsolin in Escherichia coli and dissection of actin binding sites by segmental deletion mutagenesis. J. Cell Biol. 109:593-605.
25. Weeds, A. G., J. Gooch, P. McLaughlin, B. Pope, M. Bengtsdotter, R. Karlsson. 1995. Identification of the trapped calcium in the gelsolin segment 1-actin complex: implications for the role of calcium in the control of gelsolin activity. FEBS Lett. 360:227-230.
26. Yin, H., and T. P. Stossel. 1979. Control of cytoplasmic actin gel-sol transformation by gelsolin, a calcium-dependent regulatory protein. Nature. 281:583-586.
27. 2+ control of actin filament length by gelsolin: effects of macrophage gelsolin on actin polymerization. J. Biol. Chem. 256:9693-9697.
28. Yu, F-X, D. Zhou, and H. L. Yin. 1991. Chimeric and truncated gCap39 elucidate the requirements for actin filament severing and end capping by the gelsolin family of proteins. J. Biol. Chem. 267: 14616-14621.