-
1
-
-
0027973061
-
-
T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994); M. Tewari et al., Cell 81, 801 (1995); K. Kuida et al., Nature 384, 368 (1996); E. S. Alnemri et al., Cell 87, 171 (1996); R. E. Ellis, J. Y. Yuan, H. R. Horvitz, Annu. Rev. Cell Biol. 7, 663 (1991); D. Xue, S. Shaham, H. R. Horvitz, Genes Dev. 10, 1073 (1996).
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 30761
-
-
Fernandes-Alnemri, T.1
Litwack, G.2
Alnemri, E.S.3
-
2
-
-
0028990125
-
-
T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994); M. Tewari et al., Cell 81, 801 (1995); K. Kuida et al., Nature 384, 368 (1996); E. S. Alnemri et al., Cell 87, 171 (1996); R. E. Ellis, J. Y. Yuan, H. R. Horvitz, Annu. Rev. Cell Biol. 7, 663 (1991); D. Xue, S. Shaham, H. R. Horvitz, Genes Dev. 10, 1073 (1996).
-
(1995)
Cell
, vol.81
, pp. 801
-
-
Tewari, M.1
-
3
-
-
0029956641
-
-
T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994); M. Tewari et al., Cell 81, 801 (1995); K. Kuida et al., Nature 384, 368 (1996); E. S. Alnemri et al., Cell 87, 171 (1996); R. E. Ellis, J. Y. Yuan, H. R. Horvitz, Annu. Rev. Cell Biol. 7, 663 (1991); D. Xue, S. Shaham, H. R. Horvitz, Genes Dev. 10, 1073 (1996).
-
(1996)
Nature
, vol.384
, pp. 368
-
-
Kuida, K.1
-
4
-
-
0009530192
-
-
T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994); M. Tewari et al., Cell 81, 801 (1995); K. Kuida et al., Nature 384, 368 (1996); E. S. Alnemri et al., Cell 87, 171 (1996); R. E. Ellis, J. Y. Yuan, H. R. Horvitz, Annu. Rev. Cell Biol. 7, 663 (1991); D. Xue, S. Shaham, H. R. Horvitz, Genes Dev. 10, 1073 (1996).
-
(1996)
Cell
, vol.87
, pp. 171
-
-
Alnemri, E.S.1
-
5
-
-
0025719085
-
-
T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994); M. Tewari et al., Cell 81, 801 (1995); K. Kuida et al., Nature 384, 368 (1996); E. S. Alnemri et al., Cell 87, 171 (1996); R. E. Ellis, J. Y. Yuan, H. R. Horvitz, Annu. Rev. Cell Biol. 7, 663 (1991); D. Xue, S. Shaham, H. R. Horvitz, Genes Dev. 10, 1073 (1996).
-
(1991)
Annu. Rev. Cell Biol.
, vol.7
, pp. 663
-
-
Ellis, R.E.1
Yuan, J.Y.2
Horvitz, H.R.3
-
6
-
-
0029880987
-
-
T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994); M. Tewari et al., Cell 81, 801 (1995); K. Kuida et al., Nature 384, 368 (1996); E. S. Alnemri et al., Cell 87, 171 (1996); R. E. Ellis, J. Y. Yuan, H. R. Horvitz, Annu. Rev. Cell Biol. 7, 663 (1991); D. Xue, S. Shaham, H. R. Horvitz, Genes Dev. 10, 1073 (1996).
-
(1996)
Genes Dev.
, vol.10
, pp. 1073
-
-
Xue, D.1
Shaham, S.2
Horvitz, H.R.3
-
9
-
-
1842367945
-
-
R. W. King, K. D. Lustig, P. T. Stukenberg, T. J. McGarry, M. W. Kirschner, Science 277, 973 (1997); P. T. Stukenberg et al., Curr. Biol. 7, 338 (1997).
-
(1997)
Science
, vol.277
, pp. 973
-
-
King, R.W.1
Lustig, K.D.2
Stukenberg, P.T.3
McGarry, T.J.4
Kirschner, M.W.5
-
10
-
-
0031149841
-
-
R. W. King, K. D. Lustig, P. T. Stukenberg, T. J. McGarry, M. W. Kirschner, Science 277, 973 (1997); P. T. Stukenberg et al., Curr. Biol. 7, 338 (1997).
-
(1997)
Curr. Biol.
, vol.7
, pp. 338
-
-
Stukenberg, P.T.1
-
12
-
-
1842318496
-
-
note
-
35S-labeled gelsolin. Cleavage of gelsolin was monitored by SDS-PAGE and autoradiography and occurred concurrently with caspase-3 - like activity on DEVD - 7-amino-4-methylcoumarin.
-
-
-
-
13
-
-
0022487183
-
-
D. J. Kwiatkowski et al., Nature 323, 455 (1986); M. Way and A. Weeds, J. Mol. Biol. 203, 1127 (1988); C. W. Dieffenbach, D. N. Sen Gupta, D. Krause, D. Sawzak, R. H. Silverman, J. Biol. Chem. 264, 13281 (1989).
-
(1986)
Nature
, vol.323
, pp. 455
-
-
Kwiatkowski, D.J.1
-
14
-
-
0024293322
-
-
D. J. Kwiatkowski et al., Nature 323, 455 (1986); M. Way and A. Weeds, J. Mol. Biol. 203, 1127 (1988); C. W. Dieffenbach, D. N. Sen Gupta, D. Krause, D. Sawzak, R. H. Silverman, J. Biol. Chem. 264, 13281 (1989).
-
(1988)
J. Mol. Biol.
, vol.203
, pp. 1127
-
-
Way, M.1
Weeds, A.2
-
15
-
-
0024336458
-
-
D. J. Kwiatkowski et al., Nature 323, 455 (1986); M. Way and A. Weeds, J. Mol. Biol. 203, 1127 (1988); C. W. Dieffenbach, D. N. Sen Gupta, D. Krause, D. Sawzak, R. H. Silverman, J. Biol. Chem. 264, 13281 (1989).
-
(1989)
J. Biol. Chem.
, vol.264
, pp. 13281
-
-
Dieffenbach, C.W.1
Sen Gupta, D.N.2
Krause, D.3
Sawzak, D.4
Silverman, R.H.5
-
16
-
-
1842349240
-
-
note
-
Native and cleaved gelsolin preparations were analyzed on a BioSil SEC250 column, run in 180 mM NaCI with 10 mM phosphate buffer (pH 7.4) at a flow rate of 1 ml/min, and monitored at 280 nm. Column fractions were analyzed by SDS-PAGE and Coomassie staining.
-
-
-
-
19
-
-
0029788696
-
-
W. C. Liles, P. A. Kiener, J. A. Ledbetter, A. Aruffo, S. J. Klebanoff, J. Exp. Med. 184, 429 (1996); Y. Takeda et al., Int. Immunol. 5, 691 (1993).
-
(1996)
J. Exp. Med.
, vol.184
, pp. 429
-
-
Liles, W.C.1
Kiener, P.A.2
Ledbetter, J.A.3
Aruffo, A.4
Klebanoff, S.J.5
-
20
-
-
0027326858
-
-
W. C. Liles, P. A. Kiener, J. A. Ledbetter, A. Aruffo, S. J. Klebanoff, J. Exp. Med. 184, 429 (1996); Y. Takeda et al., Int. Immunol. 5, 691 (1993).
-
(1993)
Int. Immunol.
, vol.5
, pp. 691
-
-
Takeda, Y.1
-
22
-
-
0025985999
-
-
A. G. Weeds, J. Gooch, M. Hawkins, B. Pope, M. Way, Biochem. Soc. Trans. 19, 1016 (1991).
-
(1991)
Biochem. Soc. Trans.
, vol.19
, pp. 1016
-
-
Weeds, A.G.1
Gooch, J.2
Hawkins, M.3
Pope, B.4
Way, M.5
-
23
-
-
1842308972
-
-
note
-
6) were fixed with 4% paraformaldehyde and permeabilized with ethanol/acetone. After washing with phosphate-buffered saline (PBS), cells were incubated with TdT and fluorescein-conjugated dUTP at 37°C for 1 hour. Cells were washed, and free DNA ends were visualized by fluorescent microscopy.
-
-
-
-
24
-
-
0028323294
-
-
S. W. Lowe, T. Jacks, D. E. Housman, H. E. Ruley, Proc. Natl. Acad. Sci. U.S.A. 91, 2026 (1994).
-
(1994)
Proc. Natl. Acad. Sci. U.S.A.
, vol.91
, pp. 2026
-
-
Lowe, S.W.1
Jacks, T.2
Housman, D.E.3
Ruley, H.E.4
-
25
-
-
0028914814
-
-
W. Witke et al., Cell 81, 41 (1995).
-
(1995)
Cell
, vol.81
, pp. 41
-
-
Witke, W.1
-
26
-
-
1842396785
-
-
note
-
DNA was prepared from cells by standard digestion and organic extraction. Extracted DNA (10 μg) was separated by electrophoresis in 1.5% agarose and visualized with ethidium staining.
-
-
-
-
27
-
-
1842392867
-
-
note
-
5) were treated with TNF+CHX or antibody to Fas, rinsed with PBS, and incubated in 50 mM tris-HCl (pH 7.4), 1 mM EDTA, 10 mM EGTA, and 10 μM digitonin at 37°C for 10 min. After centrifugation at 15,000g for 10 min, the lysate was incubated in DEVD-AFC (Enzyme Systems Products) for 30 min. Released AFC was measured by spectrofluorimetry with excitation at a wavelength of 380 nm and emission at 460 nm.
-
-
-
-
28
-
-
0141630973
-
-
R. U. Janicke, X. Y. Lin, F. H. H. Lee, A. G. Porter, Mol. Cell. Biol. 16, 5245 (1996).
-
(1996)
Mol. Cell. Biol.
, vol.16
, pp. 5245
-
-
Janicke, R.U.1
Lin, X.Y.2
Lee, F.H.H.3
Porter, A.G.4
-
29
-
-
0029610432
-
-
T. Mashima, M. Naito, N. Fujita, K. Noguchi, T. Tsuruo, Biochem. Biophys. Res. Commun. 217, 1185 (1995); C. Kayalar, T. Ord, M. P. Testa, L. T. Zhong, D. E. Bredesen, Proc. Natl. Acad. Sci. U.S.A. 93, 2234 (1996); Q. Song et al., ibid. 94, 157 (1997); C. Brancolini, M. Benedetti, C. Schneider, EMBO J. 14, 5179 (1995).
-
(1995)
Biochem. Biophys. Res. Commun.
, vol.217
, pp. 1185
-
-
Mashima, T.1
Naito, M.2
Fujita, N.3
Noguchi, K.4
Tsuruo, T.5
-
30
-
-
0029876247
-
-
T. Mashima, M. Naito, N. Fujita, K. Noguchi, T. Tsuruo, Biochem. Biophys. Res. Commun. 217, 1185 (1995); C. Kayalar, T. Ord, M. P. Testa, L. T. Zhong, D. E. Bredesen, Proc. Natl. Acad. Sci. U.S.A. 93, 2234 (1996); Q. Song et al., ibid. 94, 157 (1997); C. Brancolini, M. Benedetti, C. Schneider, EMBO J. 14, 5179 (1995).
-
(1996)
Proc. Natl. Acad. Sci. U.S.A.
, vol.93
, pp. 2234
-
-
Kayalar, C.1
Ord, T.2
Testa, M.P.3
Zhong, L.T.4
Bredesen, D.E.5
-
31
-
-
0031024495
-
-
T. Mashima, M. Naito, N. Fujita, K. Noguchi, T. Tsuruo, Biochem. Biophys. Res. Commun. 217, 1185 (1995); C. Kayalar, T. Ord, M. P. Testa, L. T. Zhong, D. E. Bredesen, Proc. Natl. Acad. Sci. U.S.A. 93, 2234 (1996); Q. Song et al., ibid. 94, 157 (1997); C. Brancolini, M. Benedetti, C. Schneider, EMBO J. 14, 5179 (1995).
-
(1997)
Proc. Natl. Acad. Sci. U.S.A.
, vol.94
, pp. 157
-
-
Song, Q.1
-
32
-
-
0028788309
-
-
T. Mashima, M. Naito, N. Fujita, K. Noguchi, T. Tsuruo, Biochem. Biophys. Res. Commun. 217, 1185 (1995); C. Kayalar, T. Ord, M. P. Testa, L. T. Zhong, D. E. Bredesen, Proc. Natl. Acad. Sci. U.S.A. 93, 2234 (1996); Q. Song et al., ibid. 94, 157 (1997); C. Brancolini, M. Benedetti, C. Schneider, EMBO J. 14, 5179 (1995).
-
(1995)
EMBO J.
, vol.14
, pp. 5179
-
-
Brancolini, C.1
Benedetti, M.2
Schneider, C.3
-
33
-
-
0029612323
-
-
D. A. Schafer and J. A. Cooper, Annu. Rev. Cell Dev. Biol. 11, 497 (1995); H. D. Campbell et al., Proc. Natl. Acad. Sci. U.S.A. 90, 11386 (1993); D. J. Kwiatkowski et al., unpublished data.
-
(1995)
Annu. Rev. Cell Dev. Biol.
, vol.11
, pp. 497
-
-
Schafer, D.A.1
Cooper, J.A.2
-
34
-
-
0027496510
-
-
D. A. Schafer and J. A. Cooper, Annu. Rev. Cell Dev. Biol. 11, 497 (1995); H. D. Campbell et al., Proc. Natl. Acad. Sci. U.S.A. 90, 11386 (1993); D. J. Kwiatkowski et al., unpublished data.
-
(1993)
Proc. Natl. Acad. Sci. U.S.A.
, vol.90
, pp. 11386
-
-
Campbell, H.D.1
-
35
-
-
0029612323
-
-
unpublished data
-
D. A. Schafer and J. A. Cooper, Annu. Rev. Cell Dev. Biol. 11, 497 (1995); H. D. Campbell et al., Proc. Natl. Acad. Sci. U.S.A. 90, 11386 (1993); D. J. Kwiatkowski et al., unpublished data.
-
-
-
Kwiatkowski, D.J.1
-
36
-
-
0029144456
-
-
M. Tanaka et al., Cancer Res. 55, 3228 (1995); H. L. Asch et al., ibid. 56, 4841 (1996); L. Zhang et al., Science 276, 1268 (1997).
-
(1995)
Cancer Res.
, vol.55
, pp. 3228
-
-
Tanaka, M.1
-
37
-
-
0029854630
-
-
M. Tanaka et al., Cancer Res. 55, 3228 (1995); H. L. Asch et al., ibid. 56, 4841 (1996); L. Zhang et al., Science 276, 1268 (1997).
-
(1996)
Cancer Res.
, vol.56
, pp. 4841
-
-
Asch, H.L.1
-
38
-
-
0030962307
-
-
M. Tanaka et al., Cancer Res. 55, 3228 (1995); H. L. Asch et al., ibid. 56, 4841 (1996); L. Zhang et al., Science 276, 1268 (1997).
-
(1997)
Science
, vol.276
, pp. 1268
-
-
Zhang, L.1
-
39
-
-
1842386031
-
-
note
-
35S]methionine-labeled proteins, using a coupled transcription-translation system (TNT system, Promega). We incubated 2 ml of the translation mix with 0.5 ml of active or inactivated caspase-3 (0.15 ng/ml) at 30°C for 0.5 hour. Caspase-3 was inactivated by incubation with 50 mM DEVD-fmk for 30 min before addition to the translation products. Recombinant active caspase-3 was prepared by expressing the sequence of human caspase-3 (YAMA/CPP32, a gift of V. Dixit) in E. coli (2). Purified caspase-3 (generously provided by A. L. Fear) was fully active when assayed on DEVD - 7-amino-4-methylcoumarin as previously described (3). Caspase-3 was >80% pure, consisting of 17-and 11-kD subunits, as assayed by SDS-PAGE.
-
-
-
-
42
-
-
0030052368
-
-
Rat embryo fibroblasts (REF 52) [T. Joneson, M. A. White, M. H. Wigler, D. Bar-Sagi, Science 271, 810 (1996)] were seeded on acid-treated glass cover slips, and 70% density cells were microinjected with plasmid DNA pGG-NT (encoding residues 1 through 352 of gelsolin and tagged with EYMPME epitope at the COOH-terminus) or pGG-CT (encoding residues 353 through 731 of gelsolin and tagged with EYMPME epitope at the COOH-terminus) at 0.1 μg/ ml in 100 mM Hepes (pH 7.4). A plasmid encoding green fluorescent protein (GFP) (pEGFPN1, Pro-mega) at 1 ng/ml was coinjected as a marker. Cells were fixed in 4% paraformaldehyde/PBS, permeabilized with 0.1% Triton X-100, and stained for the expression of tagged proteins, using a mouse monoclonal antibody to EYMPME (50 μg/ml) and a secondary goat anti-mouse immunoglobulin G coupled to Texas Red (Amersham). Actin filaments were visualized with tetramethyl rhodamine isothiocyanate (TRITC) - Phalloidin (Sigma).
-
(1996)
Science
, vol.271
, pp. 810
-
-
Joneson, T.1
White, M.A.2
Wigler, M.H.3
Bar-Sagi, D.4
-
44
-
-
1842357168
-
-
note
-
-/- cells. Supported by Chiron Corporation and NIH grants RO1 HL54188 and P01 HL48743 to D.J.K.
-
-
-
|