Regulation by ionizing radiation of CDC2, cyclin A, cyclin B, Thymidine Kinace, Topoisomerase IIα, and RAD51 expression in normal human diploid fibroblasts is dependent on p53/p21(Waf1)

Cell Growth and Differentiation

Volumn 9, Issue 11, 1998, Pages 887-896

  De Toledo, Sonia M ^a,b   Azzam, Edouard I ^a   Keng, Peter ^c   Laffrenier, Shelley ^b   Little, John B ^a  

^a HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^b Atomic Energy of Canada   (Canada)

^c UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CYCLE PROTEIN; CELL PROTEIN; CYCLIN A; CYCLIN B; DNA TOPOISOMERASE (ATP HYDROLYSING); MESSENGER RNA; PROTEIN P21; PROTEIN P53; THYMIDINE KINASE;

ANIMAL CELL; ARTICLE; CELL CYCLE; CONTROLLED STUDY; DIPLOIDY; DOWN REGULATION; EMBRYO; FIBROBLAST; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; IONIZING RADIATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; RADIATION DOSE;

ANTIGENS, NEOPLASM; CDC2 PROTEIN KINASE; CELLS, CULTURED; CYCLIN A; CYCLIN B; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLINS; DIPLOIDY; DNA TOPOISOMERASES, TYPE II; DNA TOPOISOMERASES, TYPE II, EUKARYOTIC; DNA-BINDING PROTEINS; FIBROBLASTS; GENE EXPRESSION REGULATION; HUMANS; ISOENZYMES; RAD51 RECOMBINASE; THYMIDINE KINASE; TUMOR SUPPRESSOR PROTEIN P53;

ANIMALIA; ATAXIA; ATAXIA TELANGIECTASIA;

EID: 0031734187     PISSN: 10449523     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (66)

1. Little, J. B. Changing views of cellular radiosensitivity. Radiat. Res., 140: 299-311, 1994.
2. Hartwell, L. H., and Weinert, T. A. Checkpoints: controls that ensure the order of cell cycle events. Science (Washington DC), 246: 629-634, 1989.
3. Scherr, C. J. Cancer cell cycles. Science (Washington DC), 275: 1672-1677, 1996.
4. 2 arrest in human cells. J. Cell Biol., 134: 963-970, 1996.
5. Kastan, M. B., Onyekwere, O., Sidransky, D., Vogelstein, B., and Craig, R. W. Participation of p53 protein in the cellular response to DNA damage. Cancer Res., 51: 6304-6311, 1991.
6. Kuerbitz, S. J., Plunkett, B. S., Walsh, W. V., and Kastan, M. B. Wild-type p53 is a cell cycle checkpoint determinant following irradiation. Proc. Natl. Acad. Sci. USA, 89: 7491-7495, 1992.
7. Lowe, S. W., Schmidt, E. M., Smith, S. W., Osborne, B. A., and Jacks, T. p53 is required for radiation-induced apoptosis in mouse thymocytes. Nature (Lond.) 362: 847-849, 1993.
8. Clarke, A. R., Purdie, C. A., Harrison, D. J., Morris, R. G., Bird, C. C., Hooper, M. L., and Wylie, A. H. Thymocyte apoptosis induced by p53-dependent and independent pathways. Nature (Lond.), 362: 849-852, 1993.
9. Barak, Y., Juven, T., Haffner, Oren, M. mdm2 expression is induced by wild type p53 activity. EMBO J., 12: 461-468, 1993.
10. El-Deiry, W. S., Tokino, T., Velculescu, V. E., Levy, D. B., Parsons, R., Trent, J. M., Lin, D., Mercer, W. E., Kinzler, K. W., and Vogelstein, B. WAF1, a potential mediator of p53 tumor suppression. Cell, 75: 817-25, 1993.
11. CIP1/WAF1 undergo normal development, but are defective in G1 checkpoint. Cell, 82: 675-684, 1995.
12. Brugarolas, J., Chandrasekaran, C., Gordon, J. I., Beach, D., Jacks, T., and Hannon, G. J. Radiation-induced cell cycle arrest compromised by p21 deficiency. Nature (Lond.), 377: 552-557, 1995.
13. Li, C., Suardet, L., and Little, J. B. Potential role of WAF/Cip/p21 as a mediator of TGF-β cytoinhibitory effect. J. Biol. Chem., 270: 4971-4974, 1994.
14. Datto, M. B., Li, Y., Panus, J. F., Howe, D. J., Xiong, Y., and Wang, X-F. Transforming growth factor β induces the cyclin-dependent kinase inhibitor p21 through a p-53-independent mechanism. Proc. Natl. Acad. Sci. USA, 92: 5545-5549, 1995.
15. Akashi, M., Hachiya, M., Osawa, Y., Spirin, K., Suzuki, G., and Koeffler, H. P. Irradiation induces WAF1 expression through a p53-independent pathway in KG-1 cells. J. Biol. Chem., 270: 19181-19187, 1995.
16. Gu, Y., Turck, C., and Morgan, D. Inhibition of cdk2 activity in vivo by an associated 20K regulatory subunit. Nature (Lond.), 366: 707-710, 1993.
17. Xiong, Y., Hannon, G. J., Zhang, H., Casso, D., Kobayashi, R., and Beach, D. p21 is a universal inhibitor of cyclin kinases. Nature (Lond.), 366: 701-704, 1993.
18. Harper, J. W., Adami, G. R., Wei, N., Keyomarsi, K., and Elledge, S. J. The p21 cdk-interacting protein cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell, 75: 805-816, 1993.
19. 1 arrest. Cell, 76: 1013-1023, 1994.
20. Nevins, J. R. E2F: a link between the Rb tumor supressor protein and viral oncoproteins. Science (Washington DC), 258: 424-429, 1992.
21. Li, R., Waga, S., Hannon, G. J., Beach, D., and Stillman, B. Differential effects by the p21 CDK inhibitor on PCNA-dependent DNA replication and repair. Nature (Lond.), 371: 534-537, 1994.
22. Zhan, Q., Liebermann, D., Alamo, I., Hollander, M. C., Ron, D., Kohn, K. W., and Fomace, A. J., Jr. The gadd and MyoD define a subset of mammalian growth arrest genes encoding acidic proteins whose expression cooperatively suppresses cell growth. Mol. Cell. Biol., 14: 2361-2371, 1994.
23. Sawyers, C. L., McLaughlin, J., Goga, A., Havilik, M., and Witte, O. The nuclear tyrosine kinase c-Abl negatively regulates cell growth. Cell, 77: 121-131, 1994.
24. Yuan, Z. M., Huang, Y., Whang, Y., Sawyers, C., Weichselbaum, R., Kharbanda, S., and Kufe, D. Role for c-abl tyrosine kinase in growth arrest response to DNA damage. Nature (Lond.), 382: 272-274, 1996.
25. Kastan, M. B., Zhan, Q., El-Deiry, W. S., Carrier, F., Jacks, T., Walsh, W. V., Plunkett, B. S., Vogelstein, B., and Fornace, A. J. A mammalian cell cycle checkpoint utilizing p53 and GADD45 is defective in ataxia telangiectasia. Cell, 71: 587-597, 1992.
26. Lu, L., and Lane, D. P. Differential induction of transcriptionally active p53 following UV or ionizing radiation: Defects in chromosome instability syndromes? Cell, 75: 765-778, 1993.
27. Nagasawa, H., and Little, J. B. Comparison of kinetics of X-ray induced cell killing in normal, ataxia telangiectasia and hereditary retinoblastoma fibroblasts. Mutat. Res., 109: 297-308, 1983.
28. Williams, K. J., Boyle, J. M., Birch, J. M., Norton, J. D., and Scott, D. Cell cycle arrest in Li-Fraumeni syndrome: a mechanism of cancer predisposition? Oncogene, 14: 277-282, 1997.
29. 1 block is abolished in human diploid fibroblasts transfected with the human papilloma virus E6 gene: Implication of the p53-CIP1/WAF1 pathway. Int. J. Oncol., 6: 233-236, 1995.
30. Tsang, N-M., Nagasawa, H., Li., C., and Little, J. B. Abrogation of p53 function by transfection of HPV 16 gene enhances the resistance of human diploid fibroblasts to ionizing radiation. Oncogene, 10: 2403-2408, 1995.
31. Azzam, E. I., de Toledo, S. M., Pykett, M. J., Nagasawa, H., and Little, J. B. CDC2 is down-regulated by ionizing radiation in a p53-dependent manner. Cell Growth Differ., 8: 1161-1169, 1997.
32. Scheffner, M., Werness, B. A., Huibregtse, J. M., Levine, A. J., and Howley, P. M. The E6 oncoprotein encoded by the human papilloma virus types 16 and 18 promotes the degradation of p53. Cell, 63: 1129-1136, 1990.
33. 2 block induced by ionizing radiation. Cancer Res., 51: 5113-5117, 1991.
34. Muschel, R. J., Zhang, H. B., and McKenna, W. G. Differential effect of ionizing radiation on the expression of cyclin A and cyclin B in Hela cells. Cancer Res., 53: 1128-1135, 1993.
35. Boothman, D. A., Davis, T. D., and Sahijdak, W. M. Enhanced expression of thymidine kinase in human cells following ionizing radiation. Int. J. Radiat. Oncol. Biol. Phys., 30: 391-398, 1994.
36. de Toledo, S. M., Azzam, E. I., Gasmann, M. K., and Mitchel, R. E. J. The use of semi-quantitative RT-PCR to study gene expression in normal human skin fibroblasts following low dose-rate irradiation. Int. J. Radiat. Biol., 67: 135-143, 1995.
37. Goswani, P. C., Roti Roti, J. L., and Hunt, C. R. The cell cycle-coupled expression of topoisomerase IIα during S phase is regulated by mRNA stability and is disrupted by heat shock or ionizing radiation. Mol. Cell. Biol., 16: 1500-1508, 1996.
38. Li, Y., Jenkins, C. W., Nichols, M. A., and Xiong, Y. Cell cycle expression and p53 regulation of the cyclin-dependent kinase inhibitor p21. Oncogene, 9: 2261-2268, 1994.
39. Wang, Q., Zambetti, G. P., and Suttle, D. P. Inhibition of DNA Topoisomerase IIα gene expression by the p53 tumor supressor. Mol. Cell. Biol., 17: 389-397, 1997.
40. 1 cyclin-dependent kinases. Genes Dev., 9: 1149-1163, 1995.
41. Dimri, G. P., Nakanishi, M., Desprez, P-Y., Smith, J. R., and Campisi, J. Inhibition of E2F activity by the cyclin-dependent protein kinase inhibitor p21 in cells expressing or lacking a functional retinoblastoma protein. Mol. Cell. Biol., 16: 2987-2997, 1996.
42. Afshari, C. A., Nichols, M. A., Xiong, Y., and Mudryj, M. A role for a p21-E2F interaction during senescent arrest of normal human fibroblasts. Cell Growth Differ., 7: 979-988, 1996.
43. Tommasi, S., and Pfeifer, G. In vivo structure of the human cdc2 promoter: release of a p130-E2F4 complex from sequences immediately upstream of the transcription initiation site coincides with induction of cdc2 expression. Mol. Cell. Biol., 15: 6901-6913, 1995.
44. 1 cell state. Mol. Cell. Biol., 16: 6965-6976, 1996.
45. 1/ S-regulatory genes. Mol. Cell. Biol., 15: 4215-4224, 1995.
46. Cobrink, D., Whyte, P., Peeper, D. S., Jacks, T., and Weinberg, R. A. Cell cycle specific association of E2F with the p130-E1A-binding protein. Genes Dev., 7: 2392-2404, 1993.
47. Ikeda, M-A., Jakoi, L., and Nevins, J. R. A unique role for the RB protein controlling E2F accumulation during cell growth and differentiation. Proc. Natl. Acad. Sci. USA, 93: 3215-20, 1996.
48. Shiyanov, P., Bagchi, S., Adami, G., Kokontis, J., Hay, N., Arroyo, Morov, A., and Raychaudhuri, P. p21 disrupts the interaction between cdk2 and the E2F-p130 complex. Mol. Cell. Biol., 16: 737-744, 1996.
49. Desdouets, C., Ory, C., Matesic, G., Soussi, T., Brechot, C., and Sobczak-Thepot, J. ATF/CREB site mediated transcriptional activation and p53 dependent repression of the cyclin A promoter. FEBS Lett., 385: 34-38, 1996.
50. Sandri, M. I., Isaacs, R. J., Ongkeko, W. M., Harris, A. L., Hickson, I. D., Broggini, M., and Vikhanskaya, F. p53 regulates the minimal promoter of the human topoisomerase IIα gene. Nucleic Acid Res., 24: 4464-4470, 1996.
51. Heuel, S., and Harlow, E. Distinct roles for cyclin-dependent kinases in cell cycle control. Science (Washington DC), 262: 2050-2054, 1993.
52. 2 cycle checkpoint in mammalian cells. Nature (Lond.), 372: 467-470, 1994.
53. 2 delay. Cancer Res., 55: 1643-1648, 1995.
54. 2 checkpoint function in cells from individuals with familial cancer syndromes. Cancer Res., 55: 1763-1773, 1995.
55. Bouffler, S. D., Kemp, C. J., Balmain, A., and Cox, R. Spontaneous and ionizing radiation-induced chromosomal abnormalities in p53-deficient mice. Cancer Res., 55: 3883-3889, 1995.
56. 2 exit of the cell cycle: role in DNA damage-induced apoptosis. Oncogene, 10: 2263-2270, 1995.
57. Waldman, T. Lengauer, C., Kinzler, K. W., and Vogelstein, B. Uncoupling of S phase and mitosis induced by anticancer agents in cells lacking p21. Nature (Lond.), 381: 713-716, 1996.
58. 2 arrest. Mol. Cell, 1: 3-11, 1997.
59. 2/M-phase transition. Mol. Cell. Biol., 18: 546-557, 1998.
60. Little, J. B. Delayed initiation of DNA synthesis in irradiated human diploid cells. Nature (Lond.), 218: 1064-1065, 1968.
61. 1 arrest and long-term induction of Cip1 in normal human fibroblasts. Genes Dev., 8: 2540-2551, 1994.
62. INK4 enrichment and the premature senescence of normal fibroblasts. Oncogene, 16: 1113-1123, 1998.
63. Datta, R., Hass, R., Gunji, H., Weichselbaum, R., and Kufe, D. Down-regulation of cell cycle control genes by ionizing radiation. Cell Growth Differ., 3: 637-644, 1992.
64. Bech-Hansen, N. T., Blattner, W. A., Sell, B. M., McKeen, E. A., Lampkin, B. C., Fraumeni, J. F., Jr., and Paterson, M. C. Transmission of in vitro radioresistance in a cancer-prone family. Lancet, 1: 1335-1337, 1981.
65. Mirzayans, R., Aubin, R. A., Bosnich, W., Blattner, W. A., and Paterson, M. C. Abnormal pattern of post-γ-ray DNA replication in radioresistant fibroblast strains from affected members of a cancer-prone family with Li-Fraumeni syndrome. Br. J. Cancer, 71: 1221-1230, 1995.
66. Chomczynski, P., and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol chloroform extraction. Anal. Biochem., 162: 156-159, 1987.