A novel and simple colorimetric method for screening Giardia intestinalis and anti-giardial activity in vitro

Parasitology

Volumn 117, Issue 3, 1998, Pages 229-234

  Kang, E W ^a   Clinch, K ^b   Furneaux, R H ^b   Harvey, J E ^b   Schofield, P J ^a   Gero, A M ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b CALLAGHAN INNOVATION   (New Zealand)

Author keywords

4 Nitrophenyl D ribofuranoside; Anti giardial; Giardia intestinalis; Metronidazole; Nucleoside hydrolase

Indexed keywords

FURAZOLIDONE; METRONIDAZOLE; TINIDAZOLE;

ANIMAL EXPERIMENT; ANTIPROTOZOAL ACTIVITY; ARTICLE; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; GIARDIA LAMBLIA; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SCREENING TEST; SPECTROPHOTOMETER; TECHNIQUE;

ANIMALS; ANTITRICHOMONAL AGENTS; CHROMOGENIC COMPOUNDS; COLORIMETRY; FURAZOLIDONE; GIARDIA LAMBLIA; GIARDIASIS; INHIBITORY CONCENTRATION 50; METRONIDAZOLE; NUCLEOTIDASES; RIBONUCLEOSIDES; SENSITIVITY AND SPECIFICITY; TINIDAZOLE;

ANIMALIA; GIARDIA INTESTINALIS; PROTOZOA;

EID: 0031708471     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0031182098002959     Document Type: Article

References (13)

1. BOREHAM, P. F. L., PHILLIPS, R. E. & SHEPHERD, R. W. (1984). The sensitivity of Giardia intestinalis to drugs in vitro. Journal of Antimicrobial Chemotherapy 14, 449-461.
2. CLINCH, K., FURNEAUX, R. H., SCHRAMM, V. L. & TYLER, P. C. (1997). Enzyme detection/assay method and substrates. PCT International Application WO 97/31008.
3. DEGANO, M., GOPAUL, D. N., SCAPIN, G., SCHRAMM, V. L. & SACCHETTINI, J. C. (1996). Three-dimensional structure of the inosine-uridine nucleoside N-ribohydrolase from Crithidia fasciculata. Biochemistry 35, 5971-5981.
4. FARBEY, M. D., REYNOLDSON, J. A. & THOMPSON, R. C. A. (1995). In vitro drug susceptibility of 29 isolates of Giardia duodenalis from humans as assessed by an adhesion assay. International Journal for Parasitology 25, 593-599.
5. GOPAUL, D. N., MEYER, S. L., DEGANO, M., SACCHETTINI, J. C. & SCHRAMM, V. L. (1996). Inosine-uridine nucleoside hydrolase from Crithidia fasciculata. Genetic characterisation, crystallization, and identification of histidine 241 as a catalytic site residue. Biochemistry 35, 5963-5970.
6. HAMMOND, D. J. & GUTTERIDGE, W. E. (1984). Purine and pyrimidine metabolism in the Trypanosomatidae. Molecular and Biochemical Parasitology 13, 243-261.
7. MAZZELLA, L. J., PARKIN, D. W., TYLER, P. C., FURNEAUX, R. H. & SCHRAMM, V. L. (1996). Mechanistic diagnoses of N-ribohydrolases and purine nucleoside phosphorylase. Journal of the American Chemical Society 118, 2111-2112.
8. PARKIN, D. W. (1996). Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei purification, specificity, and kinetic mechanism. Journal of Biological Chemistry 271, 21713-21719.
9. PARKIN, D. W., HORENSTEIN, B. A., ABDULAH, D. R., ESTUPINAN, B. & SCHRAMM, V. L. (1991). Nucleoside hydrolase from Crithidia fasciculata. Journal of Biological Chemistry 266, 20658-20665.
10. PARKIN, D. W., LIMBERG, G., TYLER, P. C., FURNEAUX, R. H., CHEN, X. Y. & SCHRAMM, V. L. (1997). Isozyme-specific transition state inhibitors for the trypanosomal nucleoside hydrolases. Biochemistry 36, 3528-3534.
11. PARKIN, D. W. & SCHRAMM, V. L. (1995). Binding modes for substrate and a proposed transition-state analogue of protozoan nucleoside hydrolase. Biochemistry 34, 13961-13966.
12. WAHL, S. M., GILMAN, R. H., O'HARE, J., KEISTER, D. B. & SPIRA, W. M. (1988). A new miniculture technique for determining in vivo antimicrobial agent sensitivity of axenically cultivated strains of Giardia lamblia. In Advances in Giardia Research, (ed. Hammond, B. R. & Wallis, P. M.), pp. 21-24. University of Calgary Press, Calgary.
13. WRIGHT, C. W., MELWANI, S. I., PHILLIPSON, J. D. & WARHURST, D. C. (1992). Determination of anti-giardial activity in vitro, by means of soluble formazan production. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 517-519.