The contribution of noncatalytic phosphate-binding subsites to the mechanism of bovine pancreatic ribonuclease A

Cellular and Molecular Life Sciences

Volumn 54, Issue 8, 1998, Pages 766-774

  Nogues M V ^a   Moussaoui, M ^a   Boix, E ^a   Vilanova, M ^b   Ribo M ^b   Cuchillo, C M ^a,c  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITY OF GIRONA   (Spain)

^c CNRS   (France)

Author keywords

Binding sites; Catalysis; Enzyme kinetics; Ribonuclease; RNA

Indexed keywords

AMINO ACID; PANCREAS ENZYME; PHOSPHATE; RIBONUCLEASE A;

ARTICLE; BINDING SITE; CATALYSIS; CATTLE; ELECTRICITY; ENZYME SUBSTRATE COMPLEX;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATALYSIS; CATTLE; HUMANS; MOLECULAR SEQUENCE DATA; PHOSPHATES; PROTEIN BINDING; RIBONUCLEASE, PANCREATIC;

BOS TAURUS; BOVINAE;

EID: 0031671322     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s000180050205     Document Type: Article

References (47)

1. Fersht A. (1985) Enzyme Structure and Mechanism, pp. 27-32, W. H. Freeman, New York
2. 2 complex at 2.3 Å resolution. J. Mol. Biol. 226: 1237-1256
3. Roberts R. J. and Halford S. E. (1993) Type II restriction enzymes. In: Nucleases, pp. 35-88, Linn S. M., Lloyd R. S. and Roberts R. J. (eds), Cold Spring Harbor Laboratory Press, Cold Spring Harbor
4. Richards F. M. and Wyckoff H. W. (1971) Bovine pancreatic ribonuclease. In: The Enzymes. vol. 4, pp. 647-806, Boyer P. D. (ed.), Academic Press, New York
5. Hartley R. W. (1997) Barnase and Barstar. In: Ribonucleases: Structures and Functions, pp. 51-100, D'Alessio G. and Riordan J. F. (eds), Academic Press, San Diego
6. Day A.G., Parsonage D., Ebel S., Brown T. and Fersht A.R. (1992) Barnase has subsites that give rise to large rate enhancements. Biochemistry 31: 6390-6395
7. Buckle A. M. and Fersht A. R. (1994) Subsite binding in an RNase-structure of a barnase tetranucleotide complex at 1.76-angstrom resolution. Biochemistry 33: 1644-1653
8. Ardelt W., Lee IL-S., Randolph G., Viera A., Mikulski S. M. and Shogen K. (1994) Enzymatic characterization of onconase, a novel ribonuclease with antitumor activity. Protein Sci. 3 (Suppl. 1): 137
9. Okabe Y., Katayama N., Iwana M., Watanabe H., Ohgi K., Irie M. et al. (1991) Comparative base specificity, stability and lectin activity of two lectins from eggs of Rana catesbeiana and R. japonica and liver ribonuclease from R. catesbeiana. J. Biochem. (Tokyo) 109: 786-790
10. Nitta K., Oyama F., Oyama R., Sekiguchi K., Kawauchi H. and Takayanagi Y. (1993) Ribonuclease activity of sialic acid-binding lectin from Rana catesbeiana eggs. Glycobiology 3: 37-45
11. Boix E., Wu Y. N., Vasandani V. M., Saxena S. K., Ardelt W., Ladner J. et al. (1996) Role of the N terminus in RNase A homologues: differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity. J. Mol. Biol. 257: 992-1007
12. Sorrentino S., Glitz D. G., Hamann K. J., Loegering D. A., Checkel J. L. and Gleich G. J. (1992) Eosinophil-derived neurotoxin and human liver ribonuclease: identity of structure and linkage of neurotoxicity to nuclease activity. J. Biol. Chem. 267: 14859-14865
13. Mosiman S. C., Newton D. L., Youle R. J. and James M. N. G. (1996) X-ray crystallographic structure of recombinant eosinophil-derived neurotoxin at 1.83 Å resolution. J. Mol. Biol. 260: 540-542
14. Slifman N. R., Loegering D. A., McKean D. J. and Gleich G. J. (1986) Ribonuclease activity associated with human eosinophil-derived neurotoxin and eosinophil cationic protein. J. Immunol. 137: 2913-2917
15. Sorrentino S. and Glitz D. G. (1991) Ribonuclease activity and substrate preference of human eosinophil cationic protein (ECP). FEBS Lett. 288: 23-26
16. Rosenberg H. F., Kimberly D. D., Tiffany H. L. and González M. (1995) Rapid evolution of a unique family of primate ribonuclease genes. Nature Genet. 10: 219-223
17. Beintema J.J. (1989) Presence of a basic amino acid residue at either position 66 or 122 is a condition for enzymic activity in the ribonuclease superfamily. FEBS Lett. 254: 1-4
18. Parés X., Nogués M. V., de Llorens R. and Cuchillo C. M. (1991) Structure and function of ribonuclease A binding subsites. In: Essays in Biochemistry, vol. 26, pp. 89-103, Tipton K. F. (ed.), Portland Press, London
19. Nogués M. V., Vilanova M. and Cuchillo C.M. (1995) Bovine pancreatic ribonuclease A as a model of an enzyme with multiple substrate binding sites. Biochim. Biophys. Acta 1253: 16-24
20. Cuchillo C. M., Vilanova M. and Nogués M.V. (1997) Pancreatic ribonucleases. In: Ribonucleases: Structures and Functions, pp. 272-304, D'Alessio G. and Riordan J. F. (eds), Academic Press, San Diego
21. Gilliland G. L. (1997) Crystallographic studies of ribonuclease complexes. In: Ribonucleases: Structures and Functions, pp. 306-341, D'Alessio G. and Riordan J. F. (eds), Academic Press, San Diego
22. McPherson A., Brayer G., Cascio D. and Williams R. (1986) The mechanism of binding of a polynucleotide chain to pancreatic ribonuclease. Science 232: 765-768
23. Fontecilla-Camps J. C., de Llorens R., le Du M. H. and Cuchillo C. M. (1994) Crystal structure of ribonuclease A-d(ApTpApApG) complex. J. Biol. Chem. 269: 21526-21531
24. 3 sites in the active site of bovine pancreatic ribonuclease A. J. Biochem. (Tokyo) 95: 751-759
25. Irie M., Mikami F., Monma K., Ohgi K.,. Watanabe H., Yamaguchi R. et al. (1984) Kinetic studies in the cleavage of oligouridylic acids and poly U by bovine pancreatic ribonuclease A. J. Biochem. (Tokyo) 96: 89-96
26. Moussaoui M., Guasch A., Boix E., Cuchillo C. M. and Nogués M. V. (1996) The role of non-catalytic binding subsites in the endonuclease activity of bovine pancreatic ribonuclease A. J. Biol. Chem. 271: 4687-4692
27. Parés X., Llorens R., Arús C. and Cuchillo C. M. (1980) The reaction of bovine pancreatic ribonuclease A with 6-chloropurine riboside 5′-monophosphate: evidence on the existence of a phosphate-binding subsite. Eur. J. Biochem. 105: 571-579
28. 1H-NMR studies on the binding subsites of bovine pancreatic ribonuclease A. Biochim. Biophys. Acta 660: 117-127
29. Arús C., Paolillo L., Llorens R., Napolitano R. and Cuchillo C. M. (1982) Evidence on the existence of a purine ligand induced conformational change in the active site of bovine pancreatic ribonuclease A studied by proton nuclear magnetic resonance spectroscopy. Biochemistry 21: 4290-4297
30. 1H-n.m.r. studies on the specificity of the interaction between bovine pancreatic ribonuclease A and dideoxynucleoside monophosphates. Int. J. Peptide Protein Res. 31: 537-543
31. 1H-n.m.r. studies on the existence of substrate binding sites in bovine pancreatic ribonuclease A. Int. J. Peptide Protein Res. 34: 66-69
32. 2 phosphate binding subsite of bovine pancreatic ribonuclease A. Biochem. J. 267: 593-599
33. Zhou H. M. and Strydom D. J. (1993) The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3′ side of uridine. Eur. J. Biochem. 217: 401-410
34. Shapiro R., Fett J. W., Strydom D. J. and Vallee B. L. (1986) Isolation and characterization of a human colon carcinoma-secreted enzyme with pancreatic ribonuclease-like activity. Biochemistry 25: 3527-3532
35. Boqué L., Coll M. G., Vilanova M, Cuchillo C. M. and Fita I. (1994) Structure of ribonuclease A derivative II at 2.1 Å resolution. J. Biol. Chem. 269: 19707-19712
36. Leonidas D. D., Shapiro R., Irons L. I., Russo N. and Acharya K. R. (1997) Crystal structures of ribonuclease A complexes with 5′-diphosphoadenosine 3′-phosphate and 5′-diphosphoadenosine 2′-phosphate at 1.7 Å resolution. Biochemistry 36: 5578-5588
37. Boix E., Nogués M. V., Schein C. H., Benner S. A. and Cuchillo C. M. (1994) Reverse transphosphorylation by ribonuclease A needs an intact p2-binding site. J. Biol. Chem. 269: 2529-2534
38. Mitsui Y., Urata Y., Torii K. and Irie M. (1978) Studies on the binding of adenylyl-3′,5′-cytidine to ribonuclease. Biochim. Biophys. Acta 535: 299-308
39. Sawada F. and Irie M. (1969) Interaction of uridine 2′(3′),5′-diphosphate with ribonuclease A and carboxymethylribonuclease A. J. Biochem. (Tokyo) 66: 415-418
40. Li J. R. and Walz G. F. (1974) A steady-state kinetic study of the ribonuclease A catalyzed hydrolysis of uridine-2′:3′(cyclic)-5′-diphosphate. Arch. Biochem. Biophys. 161: 227-233
41. Brünger A. T., Brooks C.L. and Karplus M. (1985) Active site dynamics of ribonuclease. Proc. Natl. Acad. Sci. USA 82: 8458-8462
42. de Llorens R., Arús C., Parés X. and Cuchillo C. M. (1989) Chemical and computer graphics studies on the topography of the ribonuclease A active site cleft. A model of the enzyme pentanucleotide substrate complex. Protein Eng. 2: 417-429
43. Acharya K. R., Shapiro R., Allen S. C., Riordan J. F. and Vallee B.L. (1994) Crystal structure of human angiogenin reveals the structural basis for its functional divergence from ribonuclease. Proc. Natl. Acad. Sci. USA 91: 2915-2919
44. Riordan J.F. (1997) Structure and function of angiogenin. In: Ribonucleases: Structures and Functions, pp. 446-489, D'Alessio G. and Riordan J. F. (eds), Academic Press, San Diego
45. Beintema J. J., Breukelman H. F., Carsana A. and Furia A. (1997) Evolution of vertebrate ribonucleases: ribonuclease A superfamily. In: Ribonucleases: Structures and Functions, pp. 245-269, D'Alessio G. and Riordan J. F. (eds), Academic Press, San Diego
46. Wlodaver A., Svensson L. A., Sjolin L. and Gilliland G. L. (1988) Structure of phosphate-free ribonuclease A refined at 1.26 Å. Biochemistry 27: 2705-2717
47. Roussel A., Inisan A. G. and Knoobs-Mouthy E. (1994) TURBO-FRODO (version 5.0A) Manual Biographics, Technopole de Chateau-Gombert, Marseille, France