Evaluation of a tissue homogenization technique that isolates nuclei for the in vivo single cell gel electrophoresis (comet) assay: A collaborative study by five laboratories

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 418, Issue 2-3, 1998, Pages 131-140

  Miyamae, Youichi ^a   Yamamoto, Mika ^a   Sasaki, Yu F ^b   Kobayashi, Hiroshi ^c   Igarashi Soga, Miyuki ^d   Shimoi, Kayoko ^e   Hayashi, Makoto ^f  

^a ASTELLAS PHARMA INC   (Japan)

^b Fac Chem Biol Eng   (Japan)

^c SHISEIDO RESEARCH CENTER   (Japan)

^d DAIICHI SANKYO CO   (Japan)

^e Univ S   (Japan)

^f NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

Comet assay; DNA lesion; In vivo; Mouse; Single cell gel electrophoresis (SCG) assay

Indexed keywords

DIETHYLNITROSAMINE; MESYLIC ACID METHYL ESTER;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE MARROW; CELL NUCLEUS; DNA DAMAGE; GEL ELECTROPHORESIS; GENOTOXICITY; LIVER; LUNG; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN; TECHNIQUE; TISSUE HOMOGENATE;

ANIMALS; BONE MARROW; CELL FRACTIONATION; CELL NUCLEUS; DIETHYLNITROSAMINE; DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; ETHIDIUM; FLUORESCENT DYES; KIDNEY; LIVER; LUNG; MALE; METHYL METHANESULFONATE; MICE; MICROSCOPY, FLUORESCENCE; MUTAGENS; REPRODUCIBILITY OF RESULTS; SPLEEN;

EID: 0031669821     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(98)00112-0     Document Type: Article

References (23)

1. Morita T., Asano N., Awogi T., Sasaki Y.F., Sato S., Simada H., Sutou S., Suzuki T., Wakata A., Sofuni T., Hayashi M. Evaluation of the rodent micronucleus assay in the screening of IARC carcinogens (group 1, 2A, and 2B). MMS, Mutation Res. 389:1997;3-122. The summary report of the 6th collaborative study by CSGMT/JEMS.
2. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res. 175:1988;184-191.
3. Fairbairn D.W., Olive P.L., O'neil K.L. The comet assay: a comprehensive review. Mutation Res. 339:1995;37-59.
4. Tice R.R., Andrews P.W., Singh N.P. The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair. Basic Life Sci. 53:1990;291-301.
5. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Comparative studies on cytotoxic and genotoxic effects of two organic mercury compounds in lymphocytes and gastric mucosa cells of Sprague-Dawley rats. Environ. Mol. Mutagen. 22:1994;172-180.
6. Fairbain D.W., Reyes W.A., Grigsby R.V., O'Neill K.L. Laser scanning microscopic analysis of DNA damage in frozen tissues. Cancer Lett. 76:1994;127-132.
7. Plappert U., Barthel E., Raddatz K., Seidel H.J. Early effects of benzene exposure in mice. Hematological versus genotoxic effects. Arch. Toxicol. 68:1994;284-290.
8. Monteith D.K., Vanstone J. Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage. Mutation Res. 345:1995;97-103.
9. Sirota N.P., Bezlepkin V.G., Kuznetsova E.A., Lomayeva M.G., Milonova I.N., Ravin V.K., Gaziev A.I., Bradbury R.J. Modifying effect in vivo of interferon on induction and repair of lesions in DNA of lymphoid cells of gamma-irradiated mice. Radiat. Res. 146:1996;100-105.
10. Sasaki Y.F., Tsuda S., Izumiyama F., Nishidate E. Detection of chemically induced DNA lesions in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow) using the alkaline single cell gel electrophoresis (Comet) assay. Mutation Res. 388:1997;33-44.
11. Cesarone C.F., Fugassa E., Gallo G., Voci A., Orunesu M. Collagenase perfusion of rat liver induces DNA damage and DNA repair in hepatocytes. Mutation Res. 141:1984;113-116.
12. Sasaki Y.F., Izumiyama F., Nishidate E., Matsusaka N., Tsuda S. Detection of rodent liver carcinogen genotoxicity by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow). Mutation Res. 391:1997;201-214.
13. Sasaki Y.F., Nishidate E., Izumiyama F., Matsusaka N., Tsuda S. Detection of chemical mutagens by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow). Mutation Res. 391:1997;215-231.
14. Sasaki Y.F., Izumiyama F., Nishidate E., Ohta T., Ono T., Matsusaka N., Tuda S. Simple detection of in vivo genotoxicity of pyrimethamine in rodents by the modified alkaline single-cell gel electrophoresis assay. Mutation Res. 392:1997;251-259.
15. Hayashi M., Sutou S., Shimada H., Sato S., Sasaki Y.F., Wakata A. Difference between intraperitoneal and oral gavage application in the micronucleus test. MMS, Mutation Res. 223:1989;383-386. The 3rd collaborative study by CSGMT/JEMS.
16. Higashikuni N., Morita T., Sutou S. N-nitrosodiethylamine induces micronuclei at doses below the maximum tolerated dose. MMS Commun. 2:1994;1-6.
17. Kobayashi H., Sugiyama C., Morikawa Y., Hayashi M., Sofuni T. A comparison between manual microscopic analysis and computerized image analysis in the single cell gel electrophoresis assay. MMS Commun. 3:1995;103115.
18. Wild D. Cytogenetic effects in the mouse of 17 chemical mutagens and carcinogens evaluated by the micronucleus test. Mutation Res. 56:1978;319-327.
19. Puri E.C., Müller D. Testing of hydralazine in in vivo-in vitro hepatocyte assays for UDS and stimulation of replicative DNA synthesis. Mutation Res. 218:1989;13-19.
20. Clapp N.K., Craig A.W., Toya R.E. Sr. Oncogenicity by methyl methanesulfonate in male RF mice. Science. 161:1968;913-914.
21. Frei J.V. Tumor induction by low molecular weight alkylating agents. Chem. Biol. Interactions. 3:1971;117-121.
22. Clapp N.K. Carcinogenicity of nitrosamines and methanesulphonate esters given intraperitoneally in RF mice. Int. J. Cancer. 12:1973;728-733.
23. Maitra S.C., Frei J.V. Organ-specific effects of DNA methylation by alkylating agents in the inbred swiss mouse. Chem. Biol. Interactions. 10:1975;285-293.