A comparison between ELISPOT methods for the detection of cytokine producing cells: Greater sensitivity and specificity using ELISA plates as compared to nitrocellulose membranes

Journal of Immunological Methods

Volumn 200, Issue 1-2, 1997, Pages 17-26

  Rönnelid, Johan ^a,b   Klareskog, Lars ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

ELISPOT; IFN ; IL 4; kinetic study

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 4; PYROXYLIN;

ADULT; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; INTERFERON PRODUCTION; KINETICS; NORMAL HUMAN; PRIORITY JOURNAL;

CELLS, CULTURED; COLLODION; CULTURE MEDIA; CYCLOHEXIMIDE; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; INTERFERON TYPE II; INTERLEUKIN-4; SENSITIVITY AND SPECIFICITY;

EID: 0342378189     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(96)00170-6     Document Type: Article

References (8)

1. Czerkinsky, C., Andersson, G., Ekre, H.-P., Nilsson, L.-Å., Klareskog, L. and Ouchterlony, Ö. (1988) Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells. J. Immunol. Methods 110, 29.
2. Czerkinsky, C.C., Nilsson, L.-Å., Nygren, H., Ouchterlony, Ö. and Tarkowski, A. (1983) A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J. Immunol. Methods 65, 109.
3. Howell, W.M., Warren, C.J., Cook, N.J., Cawley, M.I.D. and Smith, J.L. (1991) Detection of IL-2 at mRNA and protein level in synovial infiltrates from inflammatory arthropathies using biotinylated oligonucleotide probes in situ. Clin. Exp. Immunol. 86, 393.
4. Olsson, T., Sun, J., Hillert, J., Höjeberg, B., Ekre, H.-P., Andersson, G., Olerup, O. and Link, H. (1992) Increased numbers of T cells recognizing multiple myelin basic protein epitopes in multiple sclerosis. Eur. J. Immunol. 22, 1083.
5. Sedgwick, J.D. and Holt, P.G. (1983) A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J. Immunol. Methods 57, 301.
6. Versteegen, J.M.T., Logtenberg, T. and Ballieux, R.E. (1988) Enumeration of IFN-gamma-producing human lymphocytes by spot-ELISA. J. Immunol. Methods 111, 25.
7. Wang, W.Z., Olsson, T., Kostulas, V., Höjeberg, B., Ekre, H.P. and Link, H. (1992) Myelin antigen reactive T cells in cerebrovascular disease. Clin. Exp. Immunol. 88, 157.
8. Warren, C.J., Howell, W.M., Bhambhani, M., Cawley, M.I.D. and Smith, J.L. (1991) An investigation of T-cell subset phenotype and function in the rheumatoid synovium using in situ hybridisation for IL-2 mRNA. Immunology 72, 250.