Separation of oligonucleotides of identical size, but different base composition, by free zone capillary electrophoresis in strongly acidic, isoelectric buffers

Electrophoresis

Volumn 18, Issue 15, 1997, Pages 2915-2920

  Perego, Marilena ^a   Gelfi, Cecilia ^b   Stoyanov, Alexander V ^c   Righetti, Pier Giorgio ^a,d  

^a UNIVERSITY OF VERONA   (Italy)

^b CNR   (Italy)

^c INSTITUTE OF GENE BIOLOGY   (Russian Federation)

^d LITA   (Italy)

Author keywords

Capillary electrophoresis; Conductivity; Isoelectric buffers; Oligonucleotidcs

Indexed keywords

AMINO ACIDS; CAPILLARY ELECTROPHORESIS; METABOLISM; UREA;

ACIDIC PH VALUES; BASE COMPOSITION; CONDUCTIVITY; IMINODIACETIC ACID; ISOELECTRIC; ISOELECTRIC BUFFER; NOVEL METHODS; OLIGONUCLEOTIDCS; SINGLE BASIS; SYNTHESISED;

OLIGONUCLEOTIDES;

BUFFER; DNA FRAGMENT; OLIGONUCLEOTIDE; UREA;

ARTICLE; CAPILLARY ELECTROPHORESIS; DNA BASE COMPOSITION; DNA DETERMINATION; DNA SEQUENCE; HUMAN; NUCLEIC ACID BASE SUBSTITUTION; PH; TITRIMETRY; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0031473069     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150181532     Document Type: Article

References (23)

1. Kaplan, J. C., Delpech, M., Biologie Moleculaire et Medicine, Flammarion, Paris, 1994, pp. 550-551.
2. Cohen, J. S., Hogan, M. E., Sci. Amer. 1994 (December) pp. 50-55.
3. Wagner, R. W., Nature 1994, 372, 333-335.
4. Bourque, A. J., Cohen, A. S., J. Chromatogr. B 1994, 662, 343-349.
5. Belenky, A., Smisek, D. L., Cohen, A. S., J. Chromatogr. A 1995, 700, 137-149.
6. Cohen, A. S., Bourque, A. J., Wang, B. H., Smisek, D. L., Belenky, A., Antisense Nucleic Acid Drug Develop. 1997, 7, 13-22.
7. Gelfi, C., Perego, M., Morelli, S., Nicolin, A., Righetti, P. G., Antisense Nucleic Acid Drug Develop. 1996, 6, 47-53.
8. Gelfi, C., Perego, M., Righetti, P. G., Electrophoresis 1996, 17, 1470-1475.
9. Stoyanov, A., Gelfi, C., Righetti, P. G., Electrophoresis 1997, 18, 713-723.
10. Sealey, P. G., Southern, E. M., in: Rickwood, D., Hames, B. D. (Eds.), Gel Electrophoresis of Nucleic Acids: A Practical Approach, IRL Press, Oxford, 1982, pp. 39-76.
11. Olivera, B. M., Baine, P., Davidson, N., Biopolymers 1964, 2, 245-257.
12. Stellwagen, N. C., Gelfi, C., Righetti, P. G., Biopolymers, 1997, 42, 687-703.
13. Simò-Alfonso, E., Gelfi, C., Sebastiano, R., Citterio, A., Righetti, P. G., Electrophoresis 1996, 17, 732-737.
14. Castagnola, M., Messana, I., Cassiano, L., Rabino, R., Rossetti, D. V., Giardina, B., Electrophoresis 1995, 16, 1492-1498.
15. De Wachter, R., Fiers, W., in: Rickwood, D., Hames, B. D. (Eds.), Gel Electrophoresis of Nucleic Acids: A Practical Approach, IRL Press, Oxford, 1982, pp. 77-116.
16. Bossi, A., Righetti, P. G., Electrophoresis 1997, 18, 2012-2018.
17. Pearce, M. J., Watson, N. D., in: Pene, S. D. J., Chakraborthy, R., Epplen, J. T., Jeffrey, A. J. (Eds.), DNA Fingerprinting: State of the Science, Birkhäuser Verlag, Basel 1993, pp. 117-124.
18. Sanger, F., Nicklen, S., Coulson, A. R., Proc. Natl. Acad. Sci. USA 1977, 74, 5463-5471.
19. Jay, E., Bambara, R., Padmanabhan, R., Wu, R., Nucleic Acid Res. 1973, 1, 331-353.
20. Takigiku, R., Schneider, R. E., J. Chromatogr. 1991, 559, 247-256.
21. Cordier, Y., Roch, O., Cordier, P., Bischoff, R., J. Chromatogr. A 1994, 680, 479-489.
22. Cohen, A. S., Terabe, S., Smith, J. A., Karger, B. L., Anal. Chem. 1987, 59, 1021-1027.
23. Kasper, T. J., Melera, M., Gozel, P., Brownlee, R. G., J. Chromatogr. 1988, 458, 303-312.