A nonradioisotope approach to study the in vivo metabolism of phosphorothioate oligonucleotides

Antisense and Nucleic Acid Drug Development

Volumn 7, Issue 1, 1997, Pages 13-22

  Cohen, Aharon S ^a   Bourque, André J ^a   Wang, Bing H ^a   Smisek, David L ^a   Belenky, Alexei ^a  

^a HYBRIDON INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGODEOXYNUCLEOTIDE; COMPLEMENTARY RNA; EXONUCLEASE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG METABOLISM; FLUORESCENCE ANALYSIS; GEL ELECTROPHORESIS; HUMAN IMMUNODEFICIENCY VIRUS 1; INTRAVENOUS DRUG ADMINISTRATION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; RHESUS MONKEY; SUBCUTANEOUS DRUG ADMINISTRATION; VIRUS GENE;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; MACACA MULATTA;

EID: 0030970051     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1997.7.13     Document Type: Article

References (6)

1. AGRIS, C.H., BLAKE, K.R., MILLER, P.S., REDDY, M.P., and TS'O, P.O.P. (1986). Inhibition of vesicular stomatitis virus protein synthesis and infection by sequence-specific oligodeoxyribonucleoside methylphosphonates. Biochemistry 25, 6268-6275.
2. BOURQUE, A.J., and COHEN, A.S. (1993). Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using fast anion-exchange chromatography. J. Chromatogr. 617, 43-49.
3. BOURQUE, A.J., and COHEN, A.S. (1994). Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using direct injection fast anion-exchange chromatography and capillary gel electrophoresis. J. Chromatogr. 662, 343-349.
4. BOURQUE, A.J., SMISEK, D.L., and COHEN, A.S. (1995). Pharmacokinetic profiling of antisensc phosphorothioate DNA in humans using capillary gel electrophoresis. Presented at the 19th International Symposium on Column Liquid Chromatography and Related Techniques (Innsbruck).
5. CAMPBELL, J.M., BACON, T.A., and WICKSTROM, E. (1990). Oligodeoxynucleoside phosphorothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluid. J. Biochem. Biophys. Methods 20, 259-267.
6. ZHANG, R., LU, Z., ZHANG, X., ZHAO, H., DIASIO, R.B., LIU, T., JIANG, Z., and AGRAWAL, S. (1995b). In vivo stability and disposition of a self-stabilized oligodeoxynucleotide phosphorothioate in rats. Clin. Chem. 41, 836-843.