Analysis of antisense oligonucleotides by capillary electrophoresis, Gel-Slab electrophoresis, and HPLC: A comparison

Antisense and Nucleic Acid Drug Development

Volumn 6, Issue 1, 1996, Pages 47-53

  Gelfi, Cecilia ^a   Perego, Marilena ^b   Morelli, Susanna ^c   Nicolin, Angelo ^c   Righetti, Pier Giorgio ^b,d  

^a CNR   (Italy)

^b UNIVERSITY OF CALABRIA   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

^d LITA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE;

ARTICLE; DRUG PURIFICATION; DRUG SYNTHESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ZONE ELECTROPHORESIS;

BASE SEQUENCE; CAPILLARITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORING AGENTS; DRUG CONTAMINATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ETHIDIUM; OLIGONUCLEOTIDES, ANTISENSE; THIONUCLEOTIDES;

EID: 0029994278     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1996.6.47     Document Type: Review

References (29)

1. BOURQUE, A.J., and COHEN, A.S. (1994). Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using direct injection fast anion-exchange chromatography and capillary zone electrophoresis. J. Chromatogr. B 662, 343-349.
2. BRANDT, C.D., RAKUSAN, T.A., SISON, A.V., JOSEPHS, S.H., SAXENA, E.S., HERZOG, K.D., PARROTT, R.H., and SEVER, J.L. (1992). Detection of human immunodeficiency virus type I infection in young pediatric patients by using polymerase chain reaction and biotinylated probes. J. Clin. Microbiol. 30, 36-40.
3. BROWN, T., and BROWN, D.J.S. (1991). Modern machine-aided methods of oligodeoxyribonucleotide synthesis. In: Oligonucleotides and Analogues. F. Eckstein, ed. (IRL Press, Oxford), pp. 1-24.
4. CHIARI, M., MICHELETTI, C., NESI, M., FAZIO, M., and RIGHETTI, P.C. (1994a). Towards new formulations for polyacrylamide matrices: N-acryloylamino ethoxyethanol, a novel monomer combining high hydrophilicity with extreme hydrolytic stability. Electrophoresis 15, 177-186.
5. CHIARI, M., NESI, M., and RIGHETTI, P.G. (1994b). Capillary zone electrophoresis of DNA fragments in a novel polymer network: Poly(N-acryloylamino ethoxy ethanol). Electrophoresis 15, 616-622.
6. CICCARELLI, R.B., GUNYUZLU, P., HUANG, J., SCOTT, C., and OAKES, F. (1991). Construction of synthetic genes using PCR after automated DNA synthesis of the entire top and bottom strands. Nucleic Acids Res. 19, 6007-6013.
7. COHEN, J.S., and HOGAN, M.E. (1994). The new genetic medicines. Sci. Am. December, 50-55.
8. CROOKE, S.T., and LEBLEU, B. (1993). Antisense Research and Applications (CRC Press, Boca Raton, FL).
9. DAUM, T., ENGELIS, J.W., MAG, M., MUTH, J., LUCKINHG, S., SCHRODER, H.C., MATTHES, E., and MULLER, W. (1992). Antisense oligodeoxynucleotide-inhibitor of splicing of mRNA of human immunodeficiency virus. Intervirology 33, 65-75.
10. DeDIONISIO, L. (1993). Capillary zone electrophoresis and the analysis of DNA phosphorothioates. J. Chromatogr. 652, 101-108.
11. EFCAVITCH, W.J. (1990). The electrophoresis of synthetic oligonucleotides. In: Gel Electrophoresis of Nucleic Acids: A Practical Approach. D. Rickwood and B.B. Hames, eds. (IRL Press, Oxford), pp. 125-149.
12. GAO, W.Y., HAN, F.S., STORM, C., EGAN, W., and CHENG, Y.C. (1992). Phosphorothioate oligonucleotides are inhibitors of human DNA polymerases and RNase H. Implications for antisense technology. Mol. Pharmacol. 41, 223-229.
13. GELFI, C., ORSI, A., LEONCINI, F., and RIGHETTI, P.G. (1995). Fluidified polyacrylamides as molecular sieves in capillary zone electrophoresis of DNA fragments. J. Chromatogr. A 689, 97-105.
14. GUTTMAN, A., NELSON, R.J., and COOKE, N. (1992). Prediction of migration behaviour of oligonucleotides in CZE. J. Chromatogr. 593, 297-303.
15. HEIGER, D.N., COHEN, A.S., and KARGER, B.L. (1990). Separation of DNA restriction fragments by high-performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields. J. Chromatogr. 516, 33-42.
16. HJERTÉN, S. (1990). Zone broadening in electrophoresis with special reference to high-performance electrophoresis in capillaries: An interplay between theory and practice. Electrophoresis 11, 665-690.
17. KOOPMAN, M.B.H., De LEEUW, O.S., VAN DER ZEIJST, B.A.M., and KUSTERS, J.G. (1992). Cloning and DNA sequence analysis of a Serpulina (Treponema) hyodyseteriae gene encoding a periplastic flagellar sheet protein. Infect. Immunol. 60, 2920-2925.
18. LATTUADA, D., MAZZEI, M., MEAZZA, R., and NICOLIN, A. (1992). Therapeutic implications of antisense oligonucleotides. Int. J. Clin. Lab. Res. 21, 296-299.
19. MERRIL, M. (1987). Silver-stain detection of proteins separated by polyacrylamide gel electrophoresis. In: New Directions in Electrophoretic Methods. J.W. Jorgenson and M. Phillips, eds. (ACS Symp. Ser., Amer. Chem. Soc., Washington) 335, 74-90.
20. MORELLI, S., ALAMA, A., QUATTRONE, A., GONG, L., COPRENI, E., CANTI, G., and NICOLIN, A. (1995). Oligonucleotides induce apoptosis restricted to the t(14;18) DHL-4 cell line. Antican. Drug Design, in press.
21. NEITZEL, H., DIGWEED, M., NURNERG, P., POPPERL, A., SCHMIDT, C.A., TINSCHERT, S., and SPERLING, K. (1991). Routine applications of DNA fingerprinting with the oligonucleotide probe (CAG)5/(GTC)5. Clin. Genet. 39, 97-103.
22. PAULUS, A., GASSMANN, E., and FIELD, M.J. (1990). Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis. Electrophoresis 11, 702-708.
23. PAULUS, A., and HUSKEN, D. (1993). DNA digest analysis with capillary electrophoresis. Electrophoresis 14, 27-35.
24. PAULUS, A., and OHMS, J.I. (1990). Analysis of oligonucleotides by capillary gel electrophoresis. J. Chromatogr. 507, 113-123.
25. RIGHETTI, P.C., and CHILLEMI, F. (1978). Isoelectric focusing of peptides. J. Chromatogr. 157, 243-251.
26. RIGHETTI, P.G., and GELFI, C. (1996). Capillary electrophoresis of DNA. In: Capillary Electrophoresis In Analytical Biotechnology. P.G. Righetti, ed. (CRC Press, Boca Raton, FL) pp. 431-476.
27. SLIGHTOM, J.L., and SIEU, L.C. (1992). Direct sequencing of baculovirus genomic DNA: Sequence determination of the engineered respiratory syncytial virus chimeric FG gene. BioTechniques 13, 94-105.
28. VILENCHIK, M., BELENKY, A., and COHEN, A.S. (1994). Monitoring and anaysis of antisense DNA by capillary gel electrophoresis. J. Chromatogr. A 663, 105-113.
29. WAGNER, R.W. (1994). Gene inhibition using antisense oligonucleotides. Nature 372, 333-335.