Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of active-site lysine 115

Biochemistry

Volumn 35, Issue 1, 1996, Pages 41-46

  Highbarger, Lane A ^a   Gerlt, John A ^a,c   Kenyon, George L ^b  

^a UNIVERSITY OF MARYLAND   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOACETIC ACID; CARBOXYLYASE; LYSINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME MECHANISM; ENZYME SUBSTRATE; NONHUMAN; PKA; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CARBOXY-LYASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CLOSTRIDIUM; DNA PRIMERS; KINETICS; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SCHIFF BASES; SPECTROPHOTOMETRY;

CLOSTRIDIUM ACETOBUTYLICUM;

EID: 0030029709     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9518306     Document Type: Article

References (22)

1. Ammon, H, L., Prasad, S. M., & Gerlt, J. A. (1990) Acta Crystallogr. C47, 1476.
2. Cleland, W. W. (1979) Methods Enzymol. 63, 103.
3. Coutts, S. M. (1967) Ph.D. Dissertation, Harvard University.
4. Ellman, G. L. (1959) Arch. Biochem. Biophys. 82, 70.
5. Frey, P. A., Kokesh, F. C., & Westheimer, F. H. (1971) J. Am. Chem. Soc. 93, 7266.
6. Fridovich, I., & Westheimer, F. H. (1962) J. Am. Chem. Soc. 84, 3208.
7. Gloss, L. M., & Kirsch, J. F. (1995) Biochemistry 34, 3990.
8. Hamilton, G. A., & Westheimer, F. H. (1959) J. Am. Chem. Soc. 81, 6332.
9. Hermann, V. P., & Lemke, K. (1968) Hoppe-Seyler Z. Physiol Chem. 349, 390.
10. Kallarakal, A. T., Kozarich, J. W., Gerlt, J. A., Clifton, J. R., Petsko, G. A., & Kenyon, G. L. (1995) Biochemistry 34, 2788.
11. Kokesh, F. C., & Westheimer, F. H. (1971) J. Am. Chem. Soc. 93, 7270.
12. Landro, J. A , Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, S., Clifton, J. R., & Petsko, G. A. (1994) Biochemistry 33, 635.
13. Laursen, R. L., & Westheimer, F. H. (1966) J. Am. Chem. Soc. 88, 3426.
14. O'Leary, M. H., & Westheimer, F. H. (1968) Biochemistry 7, 913.
15. Pederson, K. (1934a) J. Phys. Chem. 8, 600.
16. Pederson, K. (1934b) J. Phys. Chem. 8, 601.
17. Petersen, D. J., & Bennett, G. N. (1993) Gene 123, 93.
18. Samiulah, M. (1984) Ph.D. Dissertation, Boston University.
19. Schmidt, D. E., Jr. & Westheimer, F. H. (1971) Biochemistry 10, 1249.
20. Tagaki, W., Guthrie, J. P., & Westheimer, F. H. (1968) Biochemistry 7, 905.
21. Westheimer, F. H. (1995) Tetrahedron 51, 3.
22. Zerner, B., Coutts, S. M., Lederer, F., Waters, H. H., & Westheimer, F. H. (1966) Biochemistry 5, 813.