Mechanism of the reaction catalyzed by acetoacetate decarboxylase. Importance of lysine 116 in determining the pKa of active-site lysine 115

Biochemistry

Volumn 35, Issue 1, 1996, Pages 41-46

  Highbarger, Lane A ^a   Gerlt, John A ^a,c   Kenyon, George L ^b  

^a University of Maryland   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOACETIC ACID; CARBOXYLYASE; LYSINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME MECHANISM; ENZYME SUBSTRATE; NONHUMAN; PKA; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CARBOXY-LYASES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CLOSTRIDIUM; DNA PRIMERS; KINETICS; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POINT MUTATION; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SCHIFF BASES; SPECTROPHOTOMETRY;

CLOSTRIDIUM ACETOBUTYLICUM;

EID: 0030029709     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9518306     Document Type: Article

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