Structure-function studies of the hammerhead ribozyme

Current Opinion in Chemical Biology

Volumn 1, Issue 4, 1997, Pages 532-536

  Verma, Sandeep ^a   Vaish, Narendra K ^a   Eckstein, Fritz ^a  

^a MAX PLANCK INSTITUTE OF EXPERIMENTAL MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGORIBONUCLEOTIDE; RIBOZYME;

CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; ENZYME SPECIFICITY; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; REVIEW; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

BASE SEQUENCE; CRYSTALLOGRAPHY, X-RAY; KINETICS; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; OLIGORIBONUCLEOTIDES; RNA, CATALYTIC; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 0031322028     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(97)80049-X     Document Type: Article

References (36)

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16. •], using a different substrate with bridging sulfur, but arrives at a different conclusion regarding the partiicipation of metal ion.
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23. Clouet-D'Orval B, Uhlenbeck OC: Kinetic characterization of two I/II format hammerhead ribozymes. RNA 1996, 2:483-491. In principle, a trans-cleaving hammerhead ribozyme can be assembled in several different constructs. More commonly, helices I and III are formed by substrate binding to give a catalytically active ribozyme. Alternatively, a substrate can also bind to form helices I and II. The latter construct was found to have different kinetics for cleavage compared to the I/III format.
24. Hertel KJ, Herschlag D, Uhlenbeck OC: Specificity of hammerhead ribozyme cleavage. EMBO J 1996, 15:3751-3757. Difference in ribozyme cleavage between wild type and mutant sequences is an important issue for biological applications. This paper addresses specificity of cleavage and protein enhancement of ribozyme catalysis.
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26. McKay DB: Structure and function of of the hammerhead ribozyme: an unfinished story. RNA 1996, 2:395-403. An excellent discussion of the effect of chemical modifications and substitutions on the hammerhead ribozyme catalytic rate is presented in this review.
27. 3-nitrogens for efficient cleavage by a hammerhead ribozyme. Biochemistry 1996, 35:6483-6490.
28. Perrachi A, Beigelman L, Usman N, Herschlag D: Rescue of a basic hammerhead ribozymes by exogenous addition of specific bases. Proc Natl Acad Sci USA 1996, 93:11522-11527. This study describes rescue of catalytic activity of abasic hammerhead ri-bozymes by a simple addition of the missing base. This observation raises the possibility of engineering ribozymes to accommodate small ligands.
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36. Birikh KR, Berlin YA, Soreq H, Eckstein F: Probing accessible sites for ribozymes on human acetylcholinesterase. RNA 1997, 3:429-437.